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Culture method of asexual cloned virus-free seedlings of sweet cherry rootstock

A cultivation method and technology for sweet cherries, applied in the field of plant tissue culture, can solve the problems of difficult planting of cherries, unstable affinity, harsh planting environment requirements, etc., and achieve the effects of improving yield, improving reliability and improving resistance.

Pending Publication Date: 2019-10-29
南京千亩休闲农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Cherry is a fruit that is very popular with consumers. It is not only high in nutrition but also small and cute in appearance. The most important thing is that it tastes good. However, the growth of cherries is affected by the environment and it is difficult to plant. Yield is cultivated by grafting
[0003] The difficulty in transplanting cherry rootstocks at the present stage is that the affinity between grafted spikes and rootstocks is unstable, resulting in uneven fruiting quality of sweet cherry plants. Not enough to adapt to different transplanting soils, poor resistance to the environment and soil, not conducive to the output of cherries
And sweet cherry plants have always been affected by viruses, which will also reduce the color quality of cherries, so cultivating a virus-free cherry rootstock with high resistance and affinity is a technical problem that those skilled in the art need to solve

Method used

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  • Culture method of asexual cloned virus-free seedlings of sweet cherry rootstock

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Experimental program
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Effect test

Embodiment 1

[0027] see figure 1 , the present invention provides a kind of technical scheme: a kind of culture method of sweet cherry rootstock asexual clone virus-free seedling, comprises the following steps:

[0028] Step 1: Seed selection, select Meizao, Black Pearl, Rabins and Molly as the training objects, cut off the 25 cm spikes at the end of the branches, cut the intercepted surface into a cone shape, and name them in turn as group A, group B, C group and D group, every group of spike quantity is 25;

[0029] Step 2: Weigh and encode, and encode each spike in Group A, Group B, Group C, and Group D, according to A1, A2, A3, A4...D1, D2, D3 and so on. After the encoding is completed, respectively Weigh each spike and record the weighing data;

[0030] Step 3: Heat treatment in groups, inserting each spike of Group A, Group B, Group C and Group D into the culture solution for soaking, and then placing them in a greenhouse at 35°C for heat treatment for 12 days;

[0031] Step 4: Se...

Embodiment 2

[0045] The present invention also provides a technical scheme: a method for cultivating asexually cloned virus-free seedlings of sweet cherry stock, comprising the following steps:

[0046] Step 1: Seed selection, select Meizao, Black Pearl, Rabins and Molly as the training objects, cut off the 25 cm spikes at the end of the branches, cut the intercepted surface into a cone shape, and name them in turn as group A, group B, C group and D group, every group of spike quantity is 27;

[0047]Step 2: Weigh and encode, and encode each spike in Group A, Group B, Group C, and Group D, according to A1, A2, A3, A4...D1, D2, D3 and so on. After the encoding is completed, respectively Weigh each spike and record the weighing data;

[0048] Step 3: Heat treatment in groups, inserting each spike of Group A, Group B, Group C and Group D into the culture solution for soaking, and then placing them in a greenhouse at 37°C for heat treatment for 13 days;

[0049] Step 4: Second weighing and s...

Embodiment 3

[0063] The present invention also provides a technical scheme: a method for cultivating asexually cloned virus-free seedlings of sweet cherry stock, comprising the following steps:

[0064] Step 1: Seed selection, select Meizao, Black Pearl, Rabins and Molly as the training objects, cut off the 25 cm spikes at the end of the branches, cut the intercepted surface into a cone shape, and name them in turn as group A, group B, C group and D group, every group of spike quantity is 28;

[0065] Step 2: Weigh and encode, and encode each spike in Group A, Group B, Group C, and Group D, according to A1, A2, A3, A4...D1, D2, D3 and so on. After the encoding is completed, respectively Weigh each spike and record the weighing data;

[0066] Step 3: Heat treatment in groups, inserting each spike of Group A, Group B, Group C and Group D into the culture solution for soaking, and then placing them in a greenhouse at 40°C for heat treatment for 13 days;

[0067] Step 4: Second weighing and ...

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Abstract

The invention discloses a culture method of asexual cloned virus-free seedlings of a sweet cherry rootstock, and relates to the technical field of plant tissue culture. The method comprises followingsteps: seed selection: Tieton, black pearl, Lapins and Bigarreav Moreav are selected as cultivation objects, 25 cm cuttings at the tail ends of branches are cut out, the cut-out surfaces are cut to beconical, and the cuttings are named group A, group B, group C and group D in sequence; weighing and coding: each cutting in the group A, the group B, the group C and the group D is coded, each codedcutting is weighed, and the weighing data is recorded. The plants are subjected to heat treatment and ear tip culture detoxification, and then subjected to chemical detoxification by cooperation withribavirin in a culture solution, and by means of combination of multiple detoxification modes, the number of viruses in tissue can be reduced, resistance of cherry plants is improved, and cherry yieldis indirectly increased.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for cultivating asexually cloned virus-free seedlings of sweet cherry rootstocks. Background technique [0002] Cherry is a very popular fruit among consumers. It is not only high in nutrition but also small and cute in appearance. The most important thing is that it tastes good. However, the growth of cherries is affected by the environment and difficult to plant. The production effect in my country has been poor. Many growers want to improve the quality of cherries. The yield is cultivated by grafting. [0003] The difficulty in transplanting cherry rootstocks at the present stage is that the affinity between grafted spikes and rootstocks is unstable, resulting in uneven fruiting quality of sweet cherry plants. It is not enough to adapt to different transplanting soils, and has poor resistance to the environment and soil, which is not conducive...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 史月龙吴延军高志红吴坤林杨艳夏鹏徐亚琴
Owner 南京千亩休闲农业发展有限公司
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