Application of n-butyl stearate

A technology of butyl octadecanoate and plants, which is applied in the field of application of butyl octadecanoate

Inactive Publication Date: 2019-11-01
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, up to now, there is no suitable method for accurately diagnosing whether plants are susceptible to diseases in the initial stage of crop susceptibility and before showing symptoms of disease.

Method used

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  • Application of n-butyl stearate
  • Application of n-butyl stearate
  • Application of n-butyl stearate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Discovery of susceptible marker compounds

[0039] (1) Collection of volatile components from healthy and susceptible Chinese rose plants

[0040] The headspace dynamic absorption method was used to collect the volatile components of Chinese rose plants that were healthy and free of diseases and insect pests. The same method was used to collect the volatile components of Chinese rose plants infected with powdery mildew. The experiment was carried out in the greenhouse used in the actual production of Chinese rose cut flowers, with a temperature of 18-42 °C, 70-100% RH, and a photoperiod of about 14 h.

[0041]During the collection process, the inlet air flow rate is 800 ml / min, and the outlet flow rate is 500 ml / min. The excess air flows out through the inevitable air leakage port, so as to ensure the high pressure inside the collection bag and prevent the outside air from entering the collection system. The collection bag is a transparent oven bag, which is pretreated...

Embodiment 2

[0057] Influence of susceptible disease grade on the content of butyl octadecanoate

[0058] Example 1 It has been found that butyl octadecanoate is an important susceptibility marker compound, which can be used to indicate whether Chinese rose plants are infected with powdery mildew. Example 2 and Example 3 further verify this. If butyl octadecanoate is indeed a marker compound for susceptibility, its content should increase with the increase of the susceptibility level (in Example 2), and increase with the prolongation of the susceptibility time (in Example 3).

[0059] In rose greenhouses, the degree of susceptibility was graded according to the leaf area covered by powdery mildew. The area of ​​1 / 16 of all leaves on a branch is covered by powdery mildew as grade I; 1 / 8 covered by powdery mildew as grade II; 1 / 4 covered by powdery mildew as grade III; Level 0 was used as a control. The volatile components were collected and analyzed by GC-MS in the same manner as in Exam...

Embodiment 3

[0062] Effect of susceptible time on the content of butyl octadecanoate

[0063] Chinese rose branches with basically the same leaf number, plant height and stem diameter were selected, 3 of which were used as healthy controls, and the others were inoculated with powdery mildew. Volatile components were collected on days 4, 7, 10, 13, and 16 after inoculation, and the collection method and GC-MS identification method were the same as in Example 2. Each time point was repeated 3 times. The results are attached Figure 5 shown.

[0064] Overall, after the Chinese rose plants were inoculated with powdery mildew, the content of butyl octadecanoate increased with the prolongation of the infection time. In healthy controls, butyl octadecanoate was not found in all three repetitions. In combination with the results of Examples 1-3, it can be confirmed that butyl octadecanoate can be used as a disease-sensitivity marker compound. If the presence of this compound is detected, it c...

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Abstract

The invention discloses an application of n-butyl stearate; the application of the n-butyl stearate is the application of n-butyl stearate in preparation of a plant biomarker of infection detection substance. The application of n-butyl stearate disclosed by the invention can be used for early diagnosis of whether rosa chinensis plants are infected with sphaerotheca pannosa or not. A compound is screened by identifying the volatile components of the rosa chinensis plants which are healthy and susceptible to diseases and identifying the volatile components of the sphaerotheca pannosa. Under thenormal condition, the healthy plant does not synthetize the n-butyl stearate, only the marker compound can be synthesized by a plant under the induction action of the sphaerotheca pannosa, and the compound is not from sphaerotheca pannosa, so that the compound can be used as the biomarker of infection compound.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to the application of butyl octadecanoate. Background technique [0002] So far, chemical pesticides are one of the main means of controlling plant diseases. However, chemical pesticides, especially chemical fungicides, will pollute the environment while eliminating plant diseases, and the well-known 3R effects of pesticides (residue residue, Resistance resistance, resurgence rampant), how to solve and avoid these problems has been a research hotspot for scientists in the field of plant protection for many years. Among them, minimizing the use of pesticides is one of the important solutions. However, according to the conventional agricultural production mode, if the use of pesticides is reduced, the economic loss will be caused because the ideal control effect cannot be achieved. [0003] In order to reduce the use of pesticides and achieve the ideal control e...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N27/62G01N1/22G01N27/622
CPCG01N30/02G01N27/622G01N1/2226G01N1/2214
Inventor 杨发忠李云仙唐敏周世萍朱国磊杨斌
Owner SOUTHWEST FORESTRY UNIVERSITY
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