A method and system for segmenting CD3-positive cell nuclei in immunohistochemical pathological images

A technique of pathological images and immunohistochemistry, applied in the field of image processing, can solve problems such as spending a lot of time and energy
CN110415255BActive Publication Date: 2021-08-20GUANGDONG GENERAL HOSPITAL
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG GENERAL HOSPITAL
Publication Date
2021-08-20

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Abstract

The invention discloses a method and a system for segmenting CD3 positive cell nuclei in immunohistochemical pathological images. The steps of the method are as follows: color deconvolution is performed on immunohistochemical pathological images to separate staining channels; the images are segmented into irregular pixels by using superpixels block, perform kmeans clustering to distinguish the image background and remove it; perform image segmentation based on morphological features to obtain a preliminarily segmented first region image L1 of the nucleus and the first image C1 to be processed; Local threshold bernsen segmentation and morphological feature segmentation to obtain the segmented second region image L2 of the nucleus and the second image C2 to be processed; the foreground of the second image C2 to be processed is marked, and the watershed algorithm is used to segment the third nucleus of the nucleus. Region image L3; the first region image L1 of the cell nucleus, the second region image L2 of the cell nucleus, and the third region image L3 of the cell nucleus constitute the cell nucleus segmentation image. The invention has high robustness and accurate segmentation, and can meet practical application requirements.
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Description

technical field

[0001] The invention relates to the technical field of image processing, in particular to a method and system for segmenting CD3-positive cell nuclei in immunohistochemical pathological images. Background technique

[0002] CD3 is an important leukocyte differentiation antigen that exists on the surface of almost all T cells and is a membrane antigen that constitutes the T cell antigen receptor (TCR). When an antigen binds to TCR, it participates in the transmission of the signal to the cell, and is an important membrane antigen involved in the discovery of various T cell functions. At present, it takes a lot of time and energy for medical staff to segment, label and count CD3 cells on pathological images. Therefore, a more accurate means of cell segmentation in pathological images is needed to reduce the pressure on medical staff. Contents of the invention

[0003] In order to reduce the burden on medical staff and realize automatic segmentation of CD3 ce...

Claims

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