Acellular matrix inside-out repair material as well as preparation method and application thereof
A technology of acellular matrix and repair materials, applied in the field of acellular matrix urethral suspension repair materials and its preparation, can solve problems such as increased postoperative pain, incisional hernia, immune rejection, and foreign body sensation in patients, and achieve good biological Compatibility, good mechanical properties, low immune effect
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Embodiment 1
[0041]1. Obtain complete porcine small intestinal submucosal tissue (SIS tissue), clean and cut radially, and use PBS solution to repeatedly rinse to remove pollutants; physically remove the mucosa, use a wooden scraper to scrape off the inside of the SIS membrane carefully and evenly The mucous membrane layer of the SIS membrane; physical removal of the sarcolemma and serosa, the use of a wooden scraper and a scalpel to remove the sarcolemma and serosa of the outer layer of the SIS membrane;
[0042] 2. Soak the SIS tissue with 0.1% trypsin for 1-2 hours to remove nucleic acid substances in the SIS tissue, and then wash it with PBS (pH=7.4) for 3 times;
[0043] 3. Use 1.8 wt% NaOH solution to inactivate the SIS tissue for 30-50 minutes to achieve the purpose of inactivating the virus in the SIS tissue; then wash the SIS tissue 3 times with PBS (pH=7.4);
[0044] 4. Put the SIS tissue into a shaker and add 0.1-0.2wt% Triton x-100 (polyethylene glycol octyl phenyl ether) to st...
Embodiment 2
[0048] 1. Select healthy young piglet skin (including dermal tissue), depilate the skin area, sterilize with benzalkonium bromide, and use a skin slicer to make the piglet dermis into a dermis with a thickness of 0.8-1.2mm;
[0049] 2. Soak the dermis with 0.1% trypsin for 2-3 hours to remove nucleic acid substances in the dermis, and then wash with PBS for 3 times;
[0050] 3. Use 1.5wt% NaOH solution to inactivate the dermal tissue for 40-60 minutes to inactivate the virus in the dermal tissue; then wash it with PBS for 3 times;
[0051] 4. Take 0.1-0.2% Triton x-100 (polyethylene glycol octyl phenyl ether) to stir and wash the dermal tissue for 16 hours, breaking the connection between lipids and between lipids and proteins. Removing residual cells in the dermal tissue, followed by washing with PBS (pH=7.4) for 3 times;
[0052] 5. Put the dermal tissue into a glutaraldehyde solution with a concentration of 0.5wt%-0.60wt% to cross-link for 2-4 hours, cut it into a shape wi...
Embodiment 3
[0055] 1. Take the bovine pericardium and cut it, the length and width are 35-40cm and 1-1.5cm respectively, and wash it in PBS solution for 3 times;
[0056] 2. Soak the pericardial tissue with 0.1% trypsin for 0.5-1 hour to remove nucleic acid substances in the pericardial tissue, and then wash it with PBS for 3 times;
[0057] 3. Use 2 wt% NaOH solution to inactivate the pericardium tissue for 30-50 minutes to achieve the purpose of inactivating the virus in the pericardium tissue. Then the SIS tissue was washed 3 times with PBS (pH=7.4);
[0058] 4. Put the pericardium tissue into a shaker and add 0.1-0.2wt% Triton x-100 (polyethylene glycol octyl phenyl ether) to stir and wash the pericardium tissue for 12 hours to break the lipid between lipids and proteins. Remove residual cells in the pericardium tissue, and then wash with PBS (pH=7.4) 3 times;
[0059] 5. Put the pericardium tissue into EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) / NHS (N-hydrox...
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