Acellular matrix inside-out repair material as well as preparation method and application thereof

A technology of acellular matrix and repair materials, applied in the field of acellular matrix urethral suspension repair materials and its preparation, can solve problems such as increased postoperative pain, incisional hernia, immune rejection, and foreign body sensation in patients, and achieve good biological Compatibility, good mechanical properties, low immune effect

Active Publication Date: 2019-11-12
广州聚明生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with synthetic materials, natural materials rarely have erosion complications, but autologous tissue is taken from the rectus abdominis fascia or fascia lata, which is complicated to operate and increases postoperative pain and incisional hernia; The samples are collected from the fascia lata of the cadaver, and there are risks of immune rejection and viral infection, and the source of samples is limited
[0004] At present, the slings and patches of polypropylene woven mesh introduced by companies such as Johnson & Johnson, Bard and Braun of Germany occupy the main market; however, the slings and patches made of these synthetic materials have poor tissue compatibility, and patients have foreign body sensation. Long-term stimulation is easy to erode the surrounding soft tissue and even penetrate the urethra or vagina, resulting in exposure and infection of the patch, affecting the treatment effect, and even requiring reoperation to remove the sling

Method used

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  • Acellular matrix inside-out repair material as well as preparation method and application thereof
  • Acellular matrix inside-out repair material as well as preparation method and application thereof
  • Acellular matrix inside-out repair material as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041]1. Obtain complete porcine small intestinal submucosal tissue (SIS tissue), clean and cut radially, and use PBS solution to repeatedly rinse to remove pollutants; physically remove the mucosa, use a wooden scraper to scrape off the inside of the SIS membrane carefully and evenly The mucous membrane layer of the SIS membrane; physical removal of the sarcolemma and serosa, the use of a wooden scraper and a scalpel to remove the sarcolemma and serosa of the outer layer of the SIS membrane;

[0042] 2. Soak the SIS tissue with 0.1% trypsin for 1-2 hours to remove nucleic acid substances in the SIS tissue, and then wash it with PBS (pH=7.4) for 3 times;

[0043] 3. Use 1.8 wt% NaOH solution to inactivate the SIS tissue for 30-50 minutes to achieve the purpose of inactivating the virus in the SIS tissue; then wash the SIS tissue 3 times with PBS (pH=7.4);

[0044] 4. Put the SIS tissue into a shaker and add 0.1-0.2wt% Triton x-100 (polyethylene glycol octyl phenyl ether) to st...

Embodiment 2

[0048] 1. Select healthy young piglet skin (including dermal tissue), depilate the skin area, sterilize with benzalkonium bromide, and use a skin slicer to make the piglet dermis into a dermis with a thickness of 0.8-1.2mm;

[0049] 2. Soak the dermis with 0.1% trypsin for 2-3 hours to remove nucleic acid substances in the dermis, and then wash with PBS for 3 times;

[0050] 3. Use 1.5wt% NaOH solution to inactivate the dermal tissue for 40-60 minutes to inactivate the virus in the dermal tissue; then wash it with PBS for 3 times;

[0051] 4. Take 0.1-0.2% Triton x-100 (polyethylene glycol octyl phenyl ether) to stir and wash the dermal tissue for 16 hours, breaking the connection between lipids and between lipids and proteins. Removing residual cells in the dermal tissue, followed by washing with PBS (pH=7.4) for 3 times;

[0052] 5. Put the dermal tissue into a glutaraldehyde solution with a concentration of 0.5wt%-0.60wt% to cross-link for 2-4 hours, cut it into a shape wi...

Embodiment 3

[0055] 1. Take the bovine pericardium and cut it, the length and width are 35-40cm and 1-1.5cm respectively, and wash it in PBS solution for 3 times;

[0056] 2. Soak the pericardial tissue with 0.1% trypsin for 0.5-1 hour to remove nucleic acid substances in the pericardial tissue, and then wash it with PBS for 3 times;

[0057] 3. Use 2 wt% NaOH solution to inactivate the pericardium tissue for 30-50 minutes to achieve the purpose of inactivating the virus in the pericardium tissue. Then the SIS tissue was washed 3 times with PBS (pH=7.4);

[0058] 4. Put the pericardium tissue into a shaker and add 0.1-0.2wt% Triton x-100 (polyethylene glycol octyl phenyl ether) to stir and wash the pericardium tissue for 12 hours to break the lipid between lipids and proteins. Remove residual cells in the pericardium tissue, and then wash with PBS (pH=7.4) 3 times;

[0059] 5. Put the pericardium tissue into EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) / NHS (N-hydrox...

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Abstract

The invention provides an acellular matrix inside-out repair material as well as a preparation method and application thereof. The method comprises the steps as follows: pretreatment, pancreatin soaking, alkali solution degreasing, Triton x-100 decellularization, crosslinking and sterilization. Due to adoption of a milder decellularization process, the collagen microstructure of the acellular matrix inside-out repair material can be saved completely, has good advantages of biocompatibility and low immunity, has good mechanical properties and can meet the condition of long-term existence in in-vivo environment. The acellular matrix inside-out repair material can effectively solve the problems that a polypropylene material has poor biocompatibility, irritates and erodes surrounding soft tissue for a long term and even penetrates urethra or vagina, cannot be degraded in vivo and even requires secondary operation to be taken out, and the acellular matrix inside-out repair material can be well applied as a urethra sling.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical biomaterials, in particular to an acellular matrix urethral suspension repair material and its preparation method and application. Background technique [0002] Stress Urinary Incontinence (SUI) refers to the involuntary leakage of urine from the external urethra when abdominal pressure increases, such as sneezing or coughing. It is a common disease in women. Epidemiological surveys at home and abroad have found that urinary incontinence The incidence rate in women is as high as 20% to 40%, and the incidence rate increases with age. However, most sick women are ashamed to see a doctor due to lack of correct understanding of the disease itself. No matter abroad or at home, the rate of seeing a doctor for sick women is less than one-third. Some patients even had serious complications, which interfered with and affected the health and quality of life of the majority of sick women ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3679A61L27/3629A61L27/3604A61L27/362A61L27/3687A61L27/50A61L2430/22
Inventor 罗云凌友杨景
Owner 广州聚明生物科技有限公司
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