Preparation and immune-enhancement use of lucid ganoderma liquid fermentation extracellular active polysaccharides

A liquid fermentation, active polysaccharide technology, applied in the direction of fermentation, organic active ingredients, medical preparations containing active ingredients, etc., can solve the problems of long production cycle and low production efficiency, and achieve the effect of good immune activity and enhanced immune activity.

Active Publication Date: 2019-11-12
SHANGHAI ACAD OF AGRI SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional ganoderma cultivation method has low production efficiency, long production cycle, and is easily affected by the environment such as soil temperature, humidity and seasonal climate, and the content of active ingredients in different batches of ganoderma is also quite different

Method used

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  • Preparation and immune-enhancement use of lucid ganoderma liquid fermentation extracellular active polysaccharides
  • Preparation and immune-enhancement use of lucid ganoderma liquid fermentation extracellular active polysaccharides
  • Preparation and immune-enhancement use of lucid ganoderma liquid fermentation extracellular active polysaccharides

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Experimental program
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Effect test

preparation example Construction

[0028] Preparation of ganoderma lucidum liquid-fermented extracellular active polysaccharide, in which slant medium: 39g of potato dextrose agar dissolved in 1L of deionized water and sterilized.

[0029] The formula of seed medium: glucose 30g / L, yeast powder 3g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 2g / L, pH natural.

[0030] The recipe of the fermentation medium:

[0031] Formula 1: Glucose 20-30g / L, yeast powder or yeast extract 2-4g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 2g / L, pH natural.

[0032] Formula 2: Galactose 20-30g / L, yeast powder or yeast extract 2-4g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 2g / L, pH natural.

[0033]Formula 3: fructose 20-30g / L, yeast powder or yeast cream 2-4g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 2g / L, pH natural.

[0034] Formula 4: Mannose 20-30g / L, yeast powder or yeast extract 2-4g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 2g / L, pH natural.

[0035] Formula 5: xylose 20-30g / L, yeast powder or yeast extract 2-4g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H...

Embodiment 1

[0038] Step 1: Ganoderma lucidum liquid fermentation culture: first transfer the Ganoderma lucidum strain to the flat PDA medium, the flat PDA medium is: glucose 30g / L, yeast powder 3g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 2g / L, natural pH, cultured in a 25-30°C incubator for 5-7 days to activate, after the mycelium covered the plate, pick about 3-6mm 2 Bacteria blocks of large and small sizes are inserted into 250mL shake flasks with a liquid volume of 100mL to carry out liquid fermentation and culture for 7-10 days to obtain a first-class seed liquid;

[0039] Step 2: Insert 10% of the inoculum into the secondary medium, the secondary seed liquid medium is: glucose 30g / L, yeast powder 3g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 2g / L, pH is natural, after cultivating for 7-10d, the secondary seed liquid is obtained, then according to the inoculum amount of 10%, it is inserted into the culture liquid of different medium formulations, cultivated on a shaker for 7-10d, and the rotati...

Embodiment 2

[0061] The seed liquid was inoculated into the fermentation medium formula 2 for cultivation, and the extracellular liquid was collected according to the method in Example 1 for alcohol precipitation treatment to obtain the large molecular weight polysaccharide component 20E.

[0062] With galactose as the carbon source and yeast powder as the nitrogen source, the yield of the 20E component obtained is 1.52g / L. The polysaccharide content of this component is 70.53%, and its monosaccharide composition and molar percentage are glucose (56.16%), Xylose (2.78%), Mannose (7.54%), Galactose (33.53%);

[0063] With glucose as carbon source and yeast extract as nitrogen source, the yield of 20E component is 1.88g / L, the polysaccharide content of this component is 66.84%, and its monosaccharide composition and mole percentage are glucose (13.12%), xylose (2.93%), mannose (2.98%), galactose (80.97%).

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Abstract

The invention discloses preparation and immune-enhancement use of lucid ganoderma liquid fermentation extracellular active polysaccharides. Lucid ganoderma strains are transferred into a flat PDA medium, then is inoculated to a liquid medium with inoculums size of 10%, and is correspondingly inoculated to a liquid medium with glucose, galactose, mannose, fructose and the like as carbon sources andyeast powder or yeast extracts as nitrogen sources for culturing, ethyl alcohol is added to make the final concentration is 20%-30%, collecting and precipitating are conducted, and drying is conducted after washing by 20%-30% of the ethyl alcohol for 2-3 times, the level of fetal alkaline phosphatase (SEAP) is determined by a QUANTI-Blue method, and the activity of NF-kappaB is activated by an evaluation sample through a Dectin-1 pathway. The results show that the lucid ganoderma fermentation extracellular polysaccharides obtained by the method all have the activity of activating NF-kappaB, and the obtainedpolysaccharides have very good immune activity.

Description

technical field [0001] The invention relates to the field of biological fermentation engineering, in particular to the preparation of ganoderma lucidum liquid fermentation extracellular active polysaccharide and its use for enhancing immunity. Background technique [0002] Ganoderma lucidum (Ganoderma spp) is a famous medicinal fungus in my country. It was regarded as the top grade in the medical monograph "Shen Nong's Materia Medica" in the Han Dynasty. Medicinal value. In recent years, with the improvement of people's requirements for the quality of life and the emphasis on health, the health care function of Ganoderma lucidum has gradually attracted people's attention. Modern pharmacological research has proved that the medicinal value of Ganoderma lucidum mainly includes immune regulation, anti-tumor, anti-radiation and auxiliary treatment of cardiovascular diseases. At present, Ganoderma lucidum has gradually become an important resource in the field of research and de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04A61K31/715A61K36/074A61P37/04C12R1/645
CPCC12P19/04A61K31/715A61K36/074A61P37/04
Inventor 刘艳芳冯杰张劲松高坤唐庆九颜梦秋周帅杨焱吴迪冯娜王金艳唐传红
Owner SHANGHAI ACAD OF AGRI SCI
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