Process for extracting and purifying glycyrrhizic acid in licorice

A technology of licorice extract and glycyrrhizic acid, which is applied in the production of organic chemistry, steroids, bulk chemicals, etc., can solve problems such as difficulty in refining glycyrrhizic acid, and achieve the effect of simple process steps

Inactive Publication Date: 2019-11-15
山东花物堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flavonoids, glycyrrhizic acid and licorice in licorice have little difference in properties. The licorice extract solution contains polysaccharides, proteins, water-soluble alkaloids, monosaccharides, starch and other substances. Glycyrrhizic acid precipitated by acid precipitation is easily wrapped with proteins and monosaccharides. and other impurities, it is not easy to refine glycyrrhizic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A process for extracting and purifying glycyrrhizic acid in licorice, comprising the following steps:

[0017] D1 preparation of licorice powder: 1000g of dried licorice is crushed into licorice powder, and the particle size of the powder is 60-100 mesh;

[0018] D2 decolorization and impurity removal: the licorice powder obtained in step D1 was reflux extracted 4 times with 80% wt ethanol in a water bath, the temperature of the water bath was 50°C, the reflux extraction time was 2h, centrifuged at 4000r / min for 10min, filtered, and the licorice was left residue;

[0019] D3 preparation of licorice extract: the licorice residue obtained in step D2 is added to the extraction solvent, the extraction solvent is an alkaline ethanol solvent, wherein the concentration of ethanol is within the range of 20%, and the volume fraction of ammonia water is 0.1%, using continuous reflux and simultaneous microwave extraction method to obtain licorice extract, which contains licorice ...

Embodiment 2

[0026] A process for extracting and purifying glycyrrhizic acid in licorice, comprising the following steps:

[0027] D1 preparation of licorice powder: the dried licorice is crushed into licorice powder, and the particle size of the powder is 60-100 mesh;

[0028] D2 decolorization and impurity removal: the licorice powder obtained in step D1 was refluxed and extracted 3 times in a water bath with 90% wt ethanol, the temperature of the water bath was 60° C., the refluxed extraction time was 1.5 h, centrifuged at 4000 r / min for 10 min, filtered, and retained Licorice residue;

[0029] D3 preparation of licorice extract: the licorice residue obtained in step D2 is added to the extraction solvent, the extraction solvent is an alkaline ethanol solvent, wherein the concentration of ethanol is within the range of 30%, and the volume fraction of ammonia water is 0.45%. extraction method to obtain licorice extract, which contains licorice flavonoids, licorice polysaccharide or glycy...

Embodiment 3

[0036] A process for extracting and purifying glycyrrhizic acid in licorice, comprising the following steps:

[0037] D1 preparation of licorice powder: the dried licorice is crushed into licorice powder, and the particle size of the powder is 60-100 mesh;

[0038] D2 decolorization and impurity removal: the licorice powder obtained in step D1 was reflux extracted twice in a water bath with 100% wt ethanol, the temperature of the water bath was 70°C, the reflux extraction time was 1h, centrifuged at 4000r / min for 10min, filtered, and the licorice was left residue;

[0039] D3 preparation of licorice extract: the licorice residue obtained in step D2 is added to the extraction solvent, the extraction solvent is an alkaline ethanol solvent, wherein the concentration of ethanol is within the range of 20%, and the volume fraction of ammonia water is 0.3%, and the extraction method is used to extract, The licorice extract is obtained, and the licorice extract contains licorice flav...

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Abstract

The invention relates to a process for extracting and purifying glycyrrhizic acid in licorice. The process comprises the following steps: D1, preparing licorice powder; D2, executing decolorization and removing impurities, executing reflux extraction in a water bath by using 80%-100% of ethanol for 2 to 4 times; D3, preparing a licorice extracting solution, wherein licorice residues obtained in step D2 are added into an extraction solvent, and the licorice extracting solution is obtained through extraction; D4, extracting glycyrrhizic acid; D5, purifying glycyrrhizic acid, recrystallizing a crude glycyrrhizic acid product with glacial acetic acid, and executing filtering to obtain the glycyrrhizic acid. The method includes the simple process steps, small molecular impurities such as monosaccharides, glycosides, polyphenols, fat and pigments are firstly removed by using the high-concentration ethanol, then components in licorice are extracted, solid substances are separated out throughstirring in the acid precipitation process, secondary dissolution is carried out by using methanol, salt is removed, and the high-purity glycyrrhizic acid is obtained.

Description

technical field [0001] The invention relates to the technical field of glycyrrhizic acid production, in particular to a process for extracting and purifying glycyrrhizic acid in licorice. Background technique [0002] Licorice is also known as Guolao, sugar beet, Ural licorice apricot, and sweet root. It is a perennial herb in the leguminous family, with strong roots and rhizomes. It is a tonic traditional Chinese medicine. Glycyrrhizic acid contains a variety of chemical components, the main components are glycyrrhizin, glycyrrhizic acid, glycyrrhetinic acid, glycyrrhizin, isoliquiritin, glycyrrhizin, and glycyrrhizin, glycyrrhizol, isoglycyrrhizol and other dozens of compounds. Among them, glycyrrhizinic acid contains hydrophilic groups and lipophilic groups, which can reduce the surface tension of aqueous solution, have strong foaming power, emulsify, fractionate, moisturize hair, soften skin, anti-wrinkle, anti-sebum, prevent pigmentation, anti-inflammatory and anti-itch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H1/08C07H15/256
CPCC07H1/08C07H15/256C07J63/008Y02P20/54
Inventor 亓云吉
Owner 山东花物堂生物科技有限公司
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