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Method for establishing regeneration system of bulb of fritillaria taipaiensis P. Y. Li with seeds as explant

A technology of Taibai Fritillaria and explants, applied in the field of Taibai Fritillaria bulb regeneration system, can solve the problems of high cost and high pollution rate, achieve the effects of reducing pollution rate and cost, increasing emergence rate, and reducing damage rate

Pending Publication Date: 2019-11-19
SANYA NANFAN SCI TECH INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been reported that the bulbs of Fritillaria fritillaria are used as explants, which have the problems of high cost and high pollution rate.

Method used

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  • Method for establishing regeneration system of bulb of fritillaria taipaiensis P. Y. Li with seeds as explant

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Embodiment Construction

[0038] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0039] The embodiment is basically as attached figure 1 Shown:

[0040] In the present embodiment, the method for the bulb regeneration system of Fritillaria taibai set up with seeds as explants includes:

[0041] Include the following steps:

[0042] S1: Obtain Taibai Fritillaria seeds;

[0043] S2: performing pretreatment on the seeds of Taibai Fritillaria to obtain pretreated seeds;

[0044] S3: sterilizing the pretreated seeds to obtain sterilized seeds;

[0045] S4: Perform seed-induced callus treatment on sterilized seeds to obtain loose granular callus. The callus-induced treatment in this embodiment is to inoculate sterilized seeds in seed germination medium, wherein the components of seed germination me...

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Abstract

The invention relates to a method for establishing a regeneration system of bulb of fritillaria taipaiensis P. Y. Li with seeds as an explant, and relates to the field of plant regeneration.The methodcomprises the following steps: S1, obtaining the seeds of the fritillaria taipaiensis P. Y. Li; S2, pretreating the seeds of the fritillaria taipaiensis P. Y. Li to obtain pretreated seeds; S3, sterilizing the pretreated seeds to obtain sterilized seeds; S4, performing seed callus induction treatment on the sterilized seeds to obtain loose granular callus; S5, putting the loose granular callus ina culture medium added with 6-BA and 2,4-D for successive proliferation for three generations to obtain a large amount of the loose granular callus, wherein one generation needs 30-40 days. The method for establishing the regeneration system of the bulb of the fritillaria taipaiensis P. Y. Li with the seeds as the explant solves the technical problem of how to improve the emergence rate of high-quality fritillaria taipaiensis P. Y. Li, and is applicable to the cultivation of the seedlings of the fritillaria taipaiensis P. Y. Li.

Description

technical field [0001] The invention relates to the field of plant regeneration, in particular to a method for a bulb regeneration system of Fritillaria taibai established by using seeds as explants. Background technique [0002] Taibai Fritillaria (Fritillaria taipaiensis P.Y.Li) is an authentic and precious medicinal material in the Taibai Mountain area of ​​the Qinba Mountains. It has a long history of being used as medicine. The sexual reproduction of Taibai Fritillaria takes 4-5 years to complete, and the germination rate of seeds is low, and it is difficult to emerge; using asexual reproduction methods, the amount of seeds used is large, the reproduction coefficient is low, and the demand is large. The market has been in short supply, resulting in high production costs. . [0003] Modern tissue culture techniques offer the possibility of mass and rapid propagation of plant seedlings. It has been reported that the bulbs of Fritillaria fritillaria are used as explants,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘扬陈冠铭李宏杨钟祥涛陈梅烹张琼李可贵任杰
Owner SANYA NANFAN SCI TECH INST
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