Method for leaf tissue culture of wild prunus domestica L.

Abstract

The invention relates to a method for culturing and propagating biological tissues, in particular to a method for leaf tissue culture of wild prunus domestica L. The method comprises the following steps: first step, selecting tender new shoots of the wild prunus domestica L., and picking off intact and damage-free leaves thereof, cleaning and then placing into a sterile clean bench for standby use; second step, treating pretreated leaf materials with 70% alcohol for 40 s, then rinsing with sterile water, then soaking with a 0.1% mercury chloride solution for 6 min, and then using the sterile water to rinse for standby use; third step, clipping disinfected leaves, and inoculating a bottle containing a medium with the leaves, wherein the formula of the medium is B5+7.5 mg / L TDZ+2.5 mg / L IBA;fourth step, after inoculation, performing dark treatment for 14 d. According to the method for the leaf tissue culture of the wild wild prunus domestica L. provided by the invention, the mortality is low,the induction rate is high, and callus state is good in the culture process, and a new conservation measure and seedling cultivation technique regulation are provided for the protection and breeding of the wild prunus domestica L.

Description

technical field [0001] The invention relates to a method for biological tissue culture and reproduction, in particular to a method for tissue culture of wild Prunus punctatus leaves. Background technique [0002] The Tianshan Mountains in western Xinjiang, my country, is one of the centers of plant origin in the world. It is rich in wild plant species. There are large areas of wild fruit tree resources distributed in this area, many of which are the origin species of cultivated fruit trees. These resources have very important utilization value for human beings. It is of great significance to carry out research on fruit tree resources, explore their origin and evolution path, and develop new fruit tree products. [0003] In 1980, Shili first discovered wild European plum (Prunus domestica L.) in the natural plants of Jiaowutuohai, Xinyuan County, Yili Prefecture, Xinjiang. In order to confirm the existence of Prunus praecox, research was carried out from the aspects of morph...

AI Technical Summary

Problems solved by technology

At present, there are few specific protection strategies and seedling raising methods for wild European plum, and there are no detailed and effective conservation measures and seedling raising technical regulations.

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