Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting of goat DNMT3B gene insertion/deletion polymorphism and application of method

A detection method and polymorphism technology, applied in DNA/RNA fragments, recombinant DNA technology, determination/inspection of microorganisms, etc., to achieve low-cost effects

Active Publication Date: 2019-11-19
YULIN UNIV
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effects of DNMT3B polymorphisms on reproductive traits in goats have not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting of goat DNMT3B gene insertion/deletion polymorphism and application of method
  • Method for detecting of goat DNMT3B gene insertion/deletion polymorphism and application of method
  • Method for detecting of goat DNMT3B gene insertion/deletion polymorphism and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting of goat DNMT3B gene insertion / deletion polymorphism and application of the method. A goat whole genome DNA to be detected is used as a template, and partial fragments of the goat DNMT3B gene are amplified by PCR; then agarose gel electrophoresis is performed, according to an electrophoreticthe result, genotypes of a goat DNMT3B gene NC_030820.1:g.61500477_61500478 locus 11-bp insertion / deletion polymorphism locus are identified. A significant correlation exists between the different genotypes of the 11-bp insertion / deletion polymorphism locus andthe litter size traits of Northern Shaanxi white cashmere goat, and a DNA marker exists to improve the litter size traits. The method for detecting the goat DNMT3B gene insertion / deletion polymorphismcan be applied to goat molecular marker assisted selection breeding to accelerate the establishment of excellent goat genetic resource population.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the rapid and accurate typing detection of goat DNMT3B gene NC_030820.1:g.61500477_61500478 11-bp insertion / deletion polymorphic site and its application in molecular marker-assisted selection (MAS) application in breeding. Background technique [0002] With the improvement of people's living standards, the society's demand for goat products continues to strengthen, but in recent years there has been a severe shortage of goat products such as mutton, goat milk, and cashmere. In terms of high-yield, high-quality and efficient goat breeding goals, the DNA marker loci that are closely related to the litter size traits of goats screened at the DNA level were detected, and according to the relationship between their gene polymorphisms and litter size traits Association, so as to speed up the establishment of goat population with good litter size traits. [0003] Mar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12Q1/6888C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125
Inventor 宋晓越惠一晴长孙伟光李增辉潘传英陈宏屈雷朱海鲸蓝贤勇李新春李陇平董书伟
Owner YULIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com