Kit for monitoring concentration of fluconazole drug in blood and detection method for kit

A kit and fluconazole technology, applied in the field of fluconazole therapeutic drug monitoring kits in blood, can solve the problems of high technical requirements for experimenters, inaccurate qualitative and quantitative, complicated preprocessing, etc., so as to reduce errors and personnel operations. The effect of error, simple and fast detection process, and reduced experimental cost

Inactive Publication Date: 2019-11-19
苏州艾迪迈医疗科技有限公司
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  • Claims
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Problems solved by technology

Previous methods have problems such as complicated pre-treatment, inaccurate qualitative and quantitative results, high cost, long analysis time, or high technical requirements for experimenters.

Method used

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  • Kit for monitoring concentration of fluconazole drug in blood and detection method for kit
  • Kit for monitoring concentration of fluconazole drug in blood and detection method for kit
  • Kit for monitoring concentration of fluconazole drug in blood and detection method for kit

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Embodiment Construction

[0035] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with illustrations and specific embodiments.

[0036] 1. Preparation of Fluconazole Therapeutic Drug Monitoring Kit:

[0037] Standard curve reagent: Fluconazole is prepared into a series of concentrations with animal serum containing 2% stabilizer and 2% plasticizer, respectively 0.5mg / L, 1.0mg / L, 3.0mg / L, 6.0mg / L, 10.0mg / L, 15.0mg / L, 1.0ml per bottle. The above components were freeze-dried and stored at the bottom of the assay bottle.

[0038] Quality Control Reagent: Among them, fluconazole was formulated with animal serum containing 2% stabilizer and 2% plasticizer to three concentrations of high, medium and low, respectively 2.0mg / L, 6.0mg / L, and 12.0mg / L, each The bottle is filled with 1.0ml. The above components were freeze-dried and stored at the bottom of the ass...

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Abstract

The invention relates to the field of medicine detection, in particular to a kit, for therapeutic drug monitoring of fluconazole in blood, developed on the basis of a multi-dimensional online solid phase extraction and liquid chromatography analysis technology, and more specifically discloses a method for carrying out accurate quantitative detection on the concentration of a fluconazole drug in blood by adopting an online solid phase extraction and multi-dimensional liquid chromatography analysis technology. The kit comprises a calibrator reagent, a quality control material reagent, treating fluid, extracting fluid, cleaning fluid and an eluent. According to the kit for therapeutic drug monitoring disclosed by the invention, only centrifugal treatment is needed for a blood sample, so thatthe repeated detection variability is small, the accuracy and the stability are high, the detection cost is low, and various methodology indicators all can meet the requirements of therapeutic drug monitoring of the fluconazole in the blood, and the kit is easy to promote.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a kit for monitoring fluconazole therapeutic drug in blood developed based on multidimensional online solid phase extraction liquid chromatography analysis technology, more specifically, it adopts online solid phase extraction and multidimensional liquid phase Chromatographic analysis technology is a method for accurate and quantitative monitoring of fluconazole drug concentration in blood. Background technique [0002] Fluconazole is a new type of triazole antifungal drug, which is used to treat a variety of systemic and superficial fungal infections, and is commonly used in meningitis and subcutaneous fungal infections caused by various fungi. Its active compound that circulates in the blood is fluconazole. The mechanism of action of this product is mainly to highly selectively interfere with the activity of fungal cytochrome P-450, thereby inhibiting the biosynthesis of ergos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/16G01N30/34G01N30/86
CPCG01N30/06G01N30/16G01N30/34G01N30/8631
Inventor 石功名何伶秀
Owner 苏州艾迪迈医疗科技有限公司
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