Newcastle disease virus antibody detection kit

A detection kit, Newcastle disease virus technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of complex criteria, non-specific identification, antibody misidentification, etc.

Active Publication Date: 2019-11-22
洛阳中科生物芯片技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both c-ELISA and b-ELISA require the use of monoclonal antibodies, which greatly increases the cost of detection; moreover, c-ELISA and b-ELISA are cumbersome to operate, take a long time to detect, and have complex criteria
On the other hand, traditional indirect ELISA uses the intact protein or recombinant protein derived from Newcastle disease virus as the coating antigen to detect antibodies in serum, and its cost is lower than that of c-ELISA and b-ELISA; however, since this method uses intact As an antigen, protein is prone to misrecognition and non-specific recognition of antibodies. Therefore, its specificity and sensitivity are not as good as c-ELISA and b-ELISA

Method used

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  • Newcastle disease virus antibody detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0049] 1. Preparation and Confirmation of Peptides

[0050] The polypeptides used in the examples were synthesized by Jill Biochemical (Shanghai) Co., Ltd. and confirmed by mass spectrometry. in,

[0051] SEQ ID NO: 1: DYIGGIGKELIVDDASDVTS.

[0052] SEQ ID NO: 2: IANCKMTTCRCVNPPGIISQ.

[0053]SEQ ID NO: 3: GSIPCQASARCPNSCVTGVY.

[0054] SEQ ID NO: 4: CSKVTETEEEDYNSAVPTRM.

[0055] SEQ ID NO: 5: KVTFDKLEKKIRSLDLSVGL.

[0056] 2. Preparation of kit (detection chip)

[0057] Kit 1

[0058] Spot the solution of the polypeptides shown in the above SEQ ID NO: 1-5 on the conventional ELISA solid phase carrier by conventional methods, and spot a chicken IgY spot as a positive quality control spot and a PB spot as a negative quality control spot point, the detection chip was prepared.

[0059] 3. Detection with kit

[0060] Put the peptide chip at room temperature for 20 minutes, observe the surface of the chip with the naked eye, and eliminate the defective peptide chip...

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Abstract

The invention relates to a Newcastle disease virus antibody detection kit. The detection kit provided by the invention comprises one or more solid carriers, and specific polypeptide or polypeptide combination independently connected to one or more solid carriers.

Description

technical field [0001] The invention mainly relates to a poultry kit and a diagnostic method. Specifically, the present invention relates to a kit and a detection method that can be used for detecting Newcastle disease antibody. Background technique [0002] Newcastle disease (ND) is a highly contagious and devastating disease caused by NDV (Newcastle disease virus, Newcastle disease virus), its mortality rate is often as high as 100%, and it is widely distributed in many countries. blight. The typical feature of this disease is respiratory tract and gastrointestinal mucosal bleeding, and people are also susceptible. The disease was first discovered in Batavia, Indonesia in 1926, and in Newtown, England in the same year, hence the name. [0003] NDV is an avian paramyxovirus type I (APMV-1) of the genus Avulavirus in the family Paramyxoviridae. The virus has an envelope structure, and its genome is an RNA virus with a single-strand negative strand without segments. It co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/68
CPCG01N33/56983G01N33/68G01N33/6854G01N2333/125
Inventor 闵亚杰刘守川
Owner 洛阳中科生物芯片技术有限公司
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