Synthetic method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan and preparation method of Newcastle disease attenuated live vaccine-loaded nanoparticles of N-2-hydroxypropyl trimethyl ammonium chloride chitosan

A technology of hydroxypropyltrimethylammonium chloride and epoxypropyltrimethylammonium chloride, which is applied in the field of synthesis and preparation of vaccine-loaded nanoparticles, can solve the problems of poor water solubility and achieve high encapsulation efficiency , simple preparation process and low production cost

Active Publication Date: 2012-05-02
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor water solubility of chitosan, its application is limited to a certain extent. Chemical modification of chitosan can improve its water solubility, biological activity and mechanical properties, and expand the use of chitosan in the above fields. Applications

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  • Synthetic method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan and preparation method of Newcastle disease attenuated live vaccine-loaded nanoparticles of N-2-hydroxypropyl trimethyl ammonium chloride chitosan
  • Synthetic method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan and preparation method of Newcastle disease attenuated live vaccine-loaded nanoparticles of N-2-hydroxypropyl trimethyl ammonium chloride chitosan
  • Synthetic method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan and preparation method of Newcastle disease attenuated live vaccine-loaded nanoparticles of N-2-hydroxypropyl trimethyl ammonium chloride chitosan

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Experimental program
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specific Embodiment approach 1

[0024] Specific embodiment one: the synthetic method of present embodiment N-2-hydroxypropyltrimethylammonium chloride chitosan is realized according to the following steps:

[0025]1. Disperse 50g of chitosan with a deacetylation degree of 85% in 200mL NaOH isobutanol solution, reflux and stir at 90-120°C for 1 hour, remove the supernatant and wash the precipitate with deionized water until neutral. Then disperse in 200mL NaOH isobutanol solution, continue to reflux and stir for 1.5 to 8 hours, remove the supernatant, and wash the precipitate with deionized water to neutrality to obtain deacetylated chitosan;

[0026] Two, the chitosan of 2~3g deacetylation process is dissolved in 100~150mL volume concentration and is 1~3% acetic acid solution, stirring and dissolving, vacuum suction filtration, filter out insoluble matter, obtain chitosan solution, then in 500 Add NaOH solution with a concentration of 0.1-2mol / L drop by drop under the stirring condition of ~1000r / min, adjust...

Embodiment 1

[0034] The synthetic method of N-2-hydroxypropyltrimethylammonium chloride chitosan is realized in the following steps:

[0035] 1. Disperse 50g of chitosan with a deacetylation degree of 85% in 200mL NaOH isobutanol solution, reflux and stir at 110°C for 1 hour, remove the supernatant and wash the precipitate with deionized water until neutral, then disperse In the isobutanol solution of 200mL NaOH, continue to reflux and stir for 1.5h, remove the supernatant and wash the precipitate with deionized water to neutrality to obtain deacetylated chitosan;

[0036] Two, the chitosan of 3g deacetylation process is dissolved in 150mL volume concentration and is 2% acetic acid solution, stirring and dissolving, vacuum suction filtration, filter off insoluble matter, obtain chitosan solution, then gradually under 750r / min stirring condition Add dropwise NaOH solution with a concentration of 0.1mol / L, adjust the pH value of the chitosan solution to 8.8 and white precipitates will separa...

specific Embodiment approach 2

[0042] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the molecular weight of chitosan in step one is 71.3. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention provides a synthetic method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan and a preparation method of Newcastle disease attenuated live vaccine-loaded nanoparticles of the N-2-hydroxypropyl trimethyl ammonium chloride chitosan, relating to a synthetic method of chitosan and a preparation method of vaccine-loaded nanoparticles of the chitosan. The synthetic method comprises the following steps of: deacelation of the chitosan; dip-treatment of the chitosan; crude preparation of the N-2-hydroxypropyl trimethyl ammonium chloride chitosan; and refined preparation of the N-2-hydroxypropyl trimethyl ammonium chloride chitosan. The preparation method comprises the following steps of: adding a Newcastle disease virus solution into an N-2-hydroxypropyl trimethyl ammonium chloride chitosan solution to obtain a solution A; adding sodium tripolyphosphate, PBS (phosphate buffer solution) and span-80 into the solution A to obtain a solution B; and centrifuging the solution B to obtain a deposit, adding PBS for suspension, adding mycose skimmed milk, and performing freeze drying to finish the preparation. The nanoparticles prepared by using the method has the advantages of easiness in control of particle size, small particle size of drug-loaded nanoparticles, high entrapment efficiency, large drug-loading quantity, mild preparation conditions, low drug toxic or side effect, long slow release time, simple preparation process, lower production cost and easiness in large-scale production.

Description

technical field [0001] The invention relates to the synthesis of chitosan and the preparation method of loaded vaccine nanoparticles. Background technique [0002] Chitosan is the product of deacetylation of chitin and is a natural renewable resource. The chemical name of chitosan is poly(2-amino-2-deoxy-β-D-glucose). Generally speaking, chitin can be called chitosan if the degree of deacetylation of chitin is above 55%. Chitin widely exists in the shells of lower animals and plants, especially arthropods. The world can biosynthesize 10 billion tons of chitin every year. It is known as the second largest natural polymer after cellulose. Therefore, chitosan is a large amount of natural resources that have not been fully utilized yet. [0003] In the process of preparing nano drug-loaded particles, it is extremely important to select the matrix material for encapsulating the drug. As the only natural polysaccharide with cations in nature, chitosan has special functions suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K9/14A61K47/36A61K39/17A61P31/14
Inventor 赵凯金政陈刚张旭
Owner HEILONGJIANG UNIV
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