Gene VII type Newcastle disease virus attenuated strain, vaccine composition and application of vaccine composition

A vaccine composition and a technology for Newcastle disease virus, which are applied in the field of attenuated strains of genotype VII Newcastle disease virus, can solve problems such as protection defects, achieve good immunogenicity, reduce the rate of virulent infection and excretion, and have a good immune effect. Effect

Active Publication Date: 2017-10-24
PU LIKE BIO ENG +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Judging from the epidemic characteristics of Newcastle disease in the past ten years, most of the virulent Newcastle disease viruses isolated clinically belong to genotype VII, while the genotypes of conventional Newcastle disease vaccine strains are mainly genotype II (such as LaSota, clone 30 , V4, etc.), the Newcastle disease epidemic strains and conventional vaccine strains are far apart in genetic distance, therefore, the protection of conventional vaccines against the current virulent strains of Newcastle disease virus still has considerable defects.

Method used

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  • Gene VII type Newcastle disease virus attenuated strain, vaccine composition and application of vaccine composition
  • Gene VII type Newcastle disease virus attenuated strain, vaccine composition and application of vaccine composition
  • Gene VII type Newcastle disease virus attenuated strain, vaccine composition and application of vaccine composition

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Embodiment approach

[0015] As a preferred embodiment of the present invention, the vaccine composition includes an immune amount of antigens of gene type VII attenuated Newcastle disease vaccine strain N7a strain or 1-60 passage cultures thereof. More preferably, the vaccine composition includes an immune amount of the gene type VII attenuated Newcastle disease vaccine strain N7a strain or its 1-55 generation cultured antigen; or an immune amount of the gene type VII Newcastle disease attenuated vaccine strain N7a strain Or the antigen of its 1-50th generation culture; or the antigen of gene Ⅶ type Ⅶ attenuated Newcastle disease vaccine strain N7a strain or its 1-45 generation culture including immune amount; or the gene Ⅶ type Ⅶ Newcastle disease attenuated virus The antigen of vaccine strain N7a strain or its 1-40th generation culture; or the antigen of gene VII type Newcastle disease attenuated vaccine strain N7a strain or its 1-35th generation culture including immunization amount; or the gene...

Embodiment 1

[0046] Example 1 Construction of Gene Type VII Newcastle Disease Attenuated Vaccine Strain

[0047] The whole genome cDNA clone (transcription vector) was constructed by segmental cloning method, and the F gene cleavage site 112R-R-Q-K-R-F117 in the genome of the isolated gene VII type virulent Newcastle disease strain PLK-N-06 strain was isolated by Overlap PCR Mutate to the attenuated strain characteristic 112G-R-Q-G-R-L117, and clone NP, P and L into the eukaryotic expression vector pCI-neo to be the helper plasmid for NDV rescue. The rescue process of NDV is to co-transfect BHK-21 cells with the whole genome cDNA clone and the helper plasmid containing the genes NP, P and L, and under the action of the relevant enzymes provided by the helper plasmid, the cDNA clone is transcribed to produce genome positive single-stranded RNA , the polymerase protein expressed by the helper plasmid wraps the positive-sense single-stranded RNA of the genome to form ribonucleoprotein complex...

Embodiment 2

[0049] The biological characteristics of embodiment 2 N7a strain Newcastle disease virus attenuated vaccine strain

[0050] The hemagglutination value (HA), the mean lethal time (MDT) of chicken embryos, the pathogenicity index (ICPI) and the median lethal dose (ELD) of 1-day-old chicks were determined for attenuated Newcastle disease virus vaccine strain N7a strain. 50 ). The specific method is as follows.

[0051] The hemagglutination value was determined according to the current "Chinese Veterinary Pharmacopoeia".

[0052] Chicken embryo mean lethal time (MDT) determination method:

[0053] (1) The freshly infected allantoic fluid was serially diluted 10 times with sterilized normal saline to 10 -6 ~10 -9 ;

[0054] (2) Inoculate 10 9-11-day-old SPF chicken embryos into the allantoic cavity of each dilution, each inoculate 0.1ml, and culture at 37°C;

[0055] (3) illuminate eggs 4 times a day, observe continuously for 7 days, and record the death time of each chicken em...

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Abstract

The invention relates to a vaccine composition. The vaccine composition comprises an immunizing dose gene VII type Newcastle disease virus attenuated strain or an antigen of a culture of the immunizing dose gene VII type Newcastle disease virus attenuated strain and a pharmaceutically acceptable carrier. The vaccine composition completely protects against the attacking toxins of the classic virus strain and the existing prevalent strains, and a vaccine prepared for protecting the relatively classic virus strain is more comprehensive. The invention also provides a vaccine prepared by using the gene VII type Newcastle disease virus attenuated strain or the antigen of the culture of the gene VII type Newcastle disease virus attenuated strain and other pathogen antigens together, and various antigen ingredients in the vaccine respectively protect the corresponding pathogens.

Description

technical field [0001] The invention relates to an attenuated strain of gene VII Newcastle disease virus, a vaccine composition prepared by the attenuated strain and its application, belonging to the field of biomedicine. Background technique [0002] Newcastle disease is a highly fatal infectious disease caused by Newcastle disease virus that can occur in a variety of poultry. Newcastle disease virus has existed in China for decades, and due to the widespread use of vaccines, the morbidity and mortality have been well controlled. However, since the early 1990s, atypical Newcastle disease has occurred from time to time. At the same time, clinically, the phenomenon of synergistic pathogenicity of Newcastle disease virus and other respiratory pathogens is also relatively common. Therefore, Newcastle disease is still one of the important diseases that currently plague the majority of farmers. one. [0003] Judging from the epidemic characteristics of Newcastle disease in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/17A61P31/14C12N7/00C12R1/93
CPCA61K39/12A61K2039/552A61K2039/55505A61K2039/55577C12N7/00C12N2760/18121C12N2760/18134
Inventor 田克恭刘武杰孙进忠张许科
Owner PU LIKE BIO ENG
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