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Compositions and methods for controlling gene expression

A plant and motif technology, applied in biochemical equipment and methods, genetic engineering, introduction of foreign genetic material using vectors, etc.

Pending Publication Date: 2019-11-26
DUKE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, a major challenge in engineering disease resistance is to overcome the associated health cost

Method used

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  • Compositions and methods for controlling gene expression
  • Compositions and methods for controlling gene expression
  • Compositions and methods for controlling gene expression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1: Revealing global translational reprogramming as a basic aspect of plant immune regulation

[0084] In the absence of specialized immune cells, the need for plants to reprogram transcription in order to shift from growth-related activities to defense is well known 1,2 . However, we know very little about the translation changes that occur during immune induction. Using the ribosome footprinting method (RF), we performed a global translation map analysis of Arabidopsis exposed to the microbial-associated molecular pattern (MAMP) elf18. We found that in the resulting pattern-triggered immunity (PTI) process, translation is tightly regulated and has a poor correlation with transcription. The identification of genes with altered translation efficiency (TE) has led to the discovery of new regulators of this immune response. Further studies of these genes have shown that the features of the mRNA sequence, rather than abundance, are the main determinants of the obser...

Embodiment 2

[0228] Example 2-Use uORF-mediated translation control to punish a wide range of plant disease resistance with minimal health penalty Applicable strategy

[0229] Since the advent of agriculture, humans have been trying their best to control plant diseases 1,2 . Research on plant immune mechanisms has led to the use of plants’ own defense genes (such as the primary immune regulatory gene NPR1 3 ) Of ectopic transcription to engineer resistant crop strategies. However, the increased resistance gained through this strategy is usually associated with a significant health penalty 4-9 The correlation makes the obtained product unsuitable for agricultural applications. To solve this problem, we seek a stricter mechanism for expressing defense proteins. According to our latest findings, key immune regulatory factors (such as TBF1 10 The translation of) is rapidly and transiently induced after pathogen attack (see attached manuscript). We developed the "TBF1-box", which consists of th...

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Abstract

The invention generally relates to compositions (including constructs, vectors, and cells) and methods of using such compositions for controlling gene expression. More specifically, the invention relates to use of R-motif sequences and / or uORF sequences to control gene expression.

Description

[0001] Cross references to related applications [0002] This application claims the benefit of US provisional patent application US 62 / 453,807 filed on February 2, 2017, which is incorporated by reference in its entirety for all purposes. [0003] Statement on Federal Government Funding of Research [0004] The present invention was completed under government funding under the grant number 5R01 GM069594-11 granted by the National Institutes of Health. The US government has certain rights in this invention. [0005] About sequence listing [0006] This application is submitted electronically through EFS-Web, including the electronically submitted sequence list submitted in txt format. The txt file contains a sequence table named "2018-02-02_5667-00424_ST25.txt" created on February 2, 2018, with a size of 155,230 bytes. The sequence listing contained in the txt file is a part of the specification and is hereby incorporated by reference in its entirety. Background technique [0007] S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82
CPCC12N15/8216C12N15/8279C12N15/8281Y02A40/146
Inventor 董欣年乔治·格林胥国勇
Owner DUKE UNIV