Microfluidic chip for simulating embryo implantation angiogenesis and preparation method and use thereof
A microfluidic chip and angiogenesis technology, applied in biochemical equipment and methods, embryonic cells, vascular endothelial cells, etc., can solve the problems of lack of angiogenesis research, lack of angiogenesis model, lack of angiogenesis research, etc., to achieve Realize large-scale, high-throughput drug screening and realize the effect of automation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0061] Example 1: Observation of the cause of angiogenesis in embryo implantation using a dual-pipe chip.
[0062] The specific preparation method is as follows:
[0063] Firstly, a double-pipe chip was prepared, and the positive control group, the negative control group, the embryo group, the endometrium group, and the embryo and endometrium co-culture group were carried out during the embryo implantation process. In the positive control group, 50ng / mL VEGF was added to the sample chamber, and the same amount of fertilized egg medium CZB was added to the sample chamber of the negative control group, and cultured for 24 hours to observe the effect on angiogenesis. In the embryo group, the embryos developed to the blastocyst stage were put into the sample chamber, and 10 embryos were placed in each sample chamber, and cultured for 24 hours. In order to prevent the liquid in the pipeline from evaporating to dryness, the liquid should be changed every 8-10 hours. In the endomet...
Embodiment 2
[0064] Example 2: Carrying out the screening of drugs related to inhibiting angiogenesis
[0065] All 19 inhibitors related to angiogenesis in the FDA drug library of the Selleck inhibitor platform were screened out. A dual-channel three-dimensional angiogenesis chip was constructed, drugs were added to the sample chamber, and co-cultured with three-dimensional vessels for 24 hours to observe the effect of drugs on vessel sprouting. Two parallel experiments were performed on the samples under each condition, and budding images of each experiment were acquired with a microscope (OLYMPUS IX73). The number of sprouts in each group was counted. Sprout lengths were measured using Image J software, and t-tests were used to determine whether there were significant differences between samples. A P value of less than 0.05 was considered to be significantly different. Screen out drugs that can significantly inhibit angiogenesis.
PUM
| Property | Measurement | Unit |
|---|---|---|
| length | aaaaa | aaaaa |
| width | aaaaa | aaaaa |
| height | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More