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A kind of SNP molecular marker of Qinchuan cattle cfl1 gene and its detection method

A molecular marker, Qinchuan cattle technology, applied in the field of molecular genetics, can solve problems such as lack of research

Active Publication Date: 2022-06-24
YANGZHOU UNIV
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Problems solved by technology

Due to the lack of research in the field of genetic variation of the CFL1 gene in Qinchuan cattle in China, the research on the function of the gene locus and the relationship between the genetic variation of the gene and economic traits (such as: meat production, growth and development, etc.) have become blank

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  • A kind of SNP molecular marker of Qinchuan cattle cfl1 gene and its detection method
  • A kind of SNP molecular marker of Qinchuan cattle cfl1 gene and its detection method
  • A kind of SNP molecular marker of Qinchuan cattle cfl1 gene and its detection method

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Embodiment Construction

[0039] The present invention uses the conserved sequence of CFL1 gene to design primer pair P to amplify the 395bp fragment of the second exon of the CFL1 gene, uses the genomic DNA of Qinchuan cattle as a template, and conducts PCR amplification, and the amplified product is sequenced to find the single point of the amplified fragment. Nucleotide polymorphism sites; conduct correlation analysis of growth traits for discovered single nucleotide polymorphism sites, and provide detection methods, making the nucleotide polymorphism of CFL1 gene a rapid and convenient detection method The molecular genetic markers of this species provide a basis for accelerating the establishment of a Qinchuan cattle population with high-quality economic traits.

[0040] a. Detection of CFL1 gene polymorphism in Qinchuan cattle

[0041] 1. Collection and processing of Qinchuan cattle blood samples

[0042] Take 10 mL of Qinchuan cattle blood sample, add 500 μL of 0.5mol / L EDTA for anticoagulation...

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Abstract

The invention belongs to the field of molecular genetics, and provides a SNP molecular marker of the CFL1 gene of Qinchuan cattle and a detection method thereof. The whole genome DNA of the Qinchuan cattle containing the CFL1 gene is used as a template, and the primer pair P is used as a primer, and PCR is performed. Amplify the CFL1 gene of Qinchuan cattle; digest the PCR amplified product with restriction endonuclease HinfI, and then perform agarose gel electrophoresis on the amplified fragment after digestion; identify the 2052nd position of the CFL1 gene of Qinchuan cattle according to the electrophoresis results base polymorphisms. Because this mutation site is closely related to the traits of Qinchuan beef (body length, chest width, chest depth, weight), and this method is a DNA level screening and detection closely related to the growth traits of Qinchuan cattle The method of molecular genetic markers, therefore, can be used in assisted selection and molecular breeding of Qinchuan cattle to speed up the breeding of Qinchuan cattle.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to using single nucleotide polymorphism (SNP) of functional gene of Qinchuan cattle as molecular genetic marker, in particular to a SNP molecular marker of Qinchuan cattle CFL1 gene and a detection method thereof. Background technique [0002] In beef cattle breeding, it is expected to achieve early selection and improve the accuracy of breeding values ​​through the selection of DNA markers that are closely related to growth traits and closely linked to quantitative traits, so as to obtain greater genetics in livestock and poultry breeding. progress. [0003] Molecular breeding, that is, molecular marker-assisted selection breeding (Molecular Mark-Assist Selection, MAS), this technology is to use DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. The combination of biology and traditional genetic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 孙雨佳徐崇杨章平
Owner YANGZHOU UNIV
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