Composite microbial preparation for degrading quinolones antibiotics and preparation method and application

A composite microbial inoculum and quinolone technology, which is applied in the field of soil organic pollutant degradation, can solve the problems of difficult technical application, low efficiency, low degradation efficiency of quinolone antibiotics, etc.

Active Publication Date: 2019-12-20
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low efficiency of existing microbial agents, the degradation efficiency of biodegradable quinolone antibiotics is low, and it is still difficult to achieve technical application

Method used

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  • Composite microbial preparation for degrading quinolones antibiotics and preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Preparation of Quinolone Antibiotic Degrading Bacteria

[0063] Take degrading bacteria: Staphylococcus caprae (Staphylococcus caprae)-NOR-36 (already in the literature "Fu Boming, Chen Liwei, Cai Tianming, et al. Isolation and screening of norfloxacin degrading bacteria NOR-36 and research on degradation characteristics [J] .Journal of Environmental Science, 2017,37(2):576-584."Open), Candida parapsilosis (Candida parapsilosis) CICC 1973, Rhodococcus (Rhodococcus sp.) CGMCC 13024, Pseudomonas sp. ) ACCC 11715, Acinetobacter (Acinetobacter sp.) ACCC 11710, Sphingomonas (Sphingomonas sp.) ACCC 11703, the individual bacteria were cultured in LB liquid medium, and the single yeast were cultured in YPD liquid medium Cultivate to the logarithmic phase at 29°C and shake at 150r / min. Adjust the concentration of each obtained degrading bacteria seed stock solution so that the concentration of each bacteria solution is about 1×10 11 cfu / mL, then mixed evenly according to th...

Embodiment 2

[0071] 1. Preparation of Quinolone Antibiotic Degrading Bacteria

[0072] Individual degrading bacteria from Staphylococcus caprae-NOR-36 and Pseudomonas sp. ACCC 11715 were cultured in LB liquid medium to the logarithmic phase, cultured at 30°C and 130r / min shaking under conditions. Then, carry out acclimatization: A, respectively get 200 μ L of cultured degrading bacteria suspension and place them in 250 mL Erlenmeyer flasks, add sterilized 100 mL liquid selective medium (norfloxacin 440 mg / L, sulfuric acid) in Erlenmeyer flasks Ammonium 5.0g / L, Potassium dihydrogen phosphate 1.0g / L, Magnesium sulfate 0.5g / L, Yeast extract 0.2g / L), the degrading bacteria were wildly domesticated for 5 days (the domestication conditions were 30°C, 130r / min shaking conditions); B. Take 200 μL of wild domestication solution and repeat step A6 times, and then in LB liquid medium, shake and cultivate for 48 hours under the conditions of 37°C and 120r / min, to prepare and form a single degrading b...

Embodiment 3

[0080] 1. Preparation of Quinolone Antibiotic Degrading Bacteria

[0081] Individual degrading bacteria in Staphylococcus caprae-NOR-36, Pseudomonas sp. ACCC 11715, and Sphingomonas sp. ACCC 11703 were cultured in LB liquid medium until In the logarithmic phase, the culture was carried out at 29°C and 150r / min shaking. Then, carry out domestication: A, respectively get 250 μ L of the degrading bacteria suspension after enrichment culture and place respectively in 250 mL Erlenmeyer flask, add sterilized 100 mL liquid selective medium (ciprofloxacin 345 mg / L) in Erlenmeyer flask , Ammonium Sulfate 5.0g / L, Potassium Dihydrogen Phosphate 1.0g / L, Magnesium Sulfate 0.5g / L, Yeast Extract 0.2g / L), the degrading bacteria were wildly domesticated for 8 days (the domestication conditions were 29°C, 150r / Min shaking conditions); B. Take 250 μL of wild domestication solution and repeat step A6 times, and then in LB liquid medium, shake and cultivate for 60 hours under the conditions of 3...

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Abstract

The invention belongs to the technical field of degradation of soil organic pollutants, and discloses a composite microbial preparation for degrading quinolones antibiotics and a preparation method and application. The preparation method comprises the steps of mixing quinolones antibiotics degradation bacterial liquid with energy supply bacterium bacterial liquid, magnetic medium bacterium bacterial liquid, a surfactant and a fermentation medium to obtain mixed liquid; and performing fermentation to obtain the composite microbial preparation for degrading the quinolones antibiotics. Energy supply bacteria which are adopted enable the composite microbial preparation to have high colonization and multiplication capacity in in-place edatope. The surfactant and the magnetic medium bacteria, which are adopted, enable the composite microbial preparation to have low moisture tension and antibiotics polarity. The prepared composite microbial preparation is high in activity, and high in environmental suitability. The composite microbial preparation is used for degradation of the quinolones antibiotics in soil, and is broad in scope of application and high in degradation efficiency.

Description

technical field [0001] The invention belongs to the technical field of degradation of soil organic pollutants, and in particular relates to a compound microbial bacterial agent for degrading quinolone antibiotics, a preparation method and application thereof. Background technique [0002] Quinolones (QNs) are artificially synthesized antibacterial drugs containing 4-quinolone basic structure. They have the characteristics of broad antibacterial spectrum, strong antibacterial activity, no cross-resistance with other antibacterial drugs, and small toxic and side effects. They are clinical prescriptions. The rate is second only to β-lactams universal anti-infective drugs for humans and animals. China is a big consumer of quinolone antibiotics. A large number of quinolone antibiotics finally enter the soil system through human or animal excretion, pharmaceutical sewage discharge, solid waste dumping and other channels, resulting in the detection of quinolone antibiotics in my c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16B09C1/10C12R1/44C12R1/72C12R1/38C12R1/15C12R1/07C12R1/13C12R1/39C12R1/01
CPCB09C1/10B09C1/105C12N1/16C12N1/20
Inventor 徐培智蒋瑞萍李夏孙丽丽解开治李文英顾文杰卢钰升卢廷超
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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