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Application of arginine for increasing yield of monacolin K produced by monascus purpureus

A technology of Monascus purpureus, arginine, applied in the direction of microorganism-based methods, adding compounds to stimulate growth, microorganisms, etc., can solve problems such as inability to determine well

Active Publication Date: 2019-12-20
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different experimental strains, treatment methods and detection methods, it is not possible to determine which additive is the best inducer

Method used

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  • Application of arginine for increasing yield of monacolin K produced by monascus purpureus
  • Application of arginine for increasing yield of monacolin K produced by monascus purpureus
  • Application of arginine for increasing yield of monacolin K produced by monascus purpureus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Experiments on the Influence of Arginine on the Production of Monacolin K

[0024] Experimental steps:

[0025] In the early stage of the experiment, by consulting the literature, the addition amount of arginine was selected as 0.5%, 1% and 1.5%. Through the liquid fermentation of Monascus bacterium and the detection of monacolin K by HPLC, it was found that the production of monacolin K decreased with the increase of the addition amount of arginine. , so 0.5% was used as the standard, and 0.4%, 0.5% and 0.6% arginine were selected for addition, and it was found that when the arginine addition was 0.6%, the yield of monacolin K was the highest. Afterwards, the changes of some other physiological indicators after the addition of arginine were further detected, such as monascus pigment, pH value, biomass and scanning electron microscope analysis.

[0026] Strain activation: the M1 strain was cultured on PDA medium at 30°C for 3 days and activated for 2 generati...

Embodiment 2

[0052] Embodiment 2: Effect comparison of glutamic acid and arginine:

[0053] In the prior art, in the article "Analysis of the Promoting Effect of Ten Kinds of Additives on the Output of Monascus monacolin K", yam powder, orange peel powder, yeast liquid, yeast supernatant, Yeast broken liquid, broken yeast liquid, leucine, glutamic acid, ethanol and other substances were used to determine the yield of secondary metabolites of Monascus in different media. The results showed that the addition of glutamic acid could significantly (p < 0.01) increase the yield of monacolin K by 5.60 times.

[0054] The difference between glutamic acid and arginine is that although the effect of glutamic acid is better than that of arginine, the disadvantage of glutamic acid is that although it can significantly increase the output of monacolin K, the output of monascus pigment is lower than that of the original The culture medium is reduced, and arginine can improve the yield of monacolin K wh...

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Abstract

The invention discloses a fermentation medium for producing monacolin K from monascus purpureus; and the fermentation medium comprises arginine as an ingredient. According to the invention, the arginine is added into the fermentation medium, when the addition amount is 0.6%, the yield of the monacolin K is increased by 3.65 times on day 8 and the yield of the monacolin K is increased by 2.89 timeson day 15 compared with the yield of a primary fermentation medium.

Description

technical field [0001] The invention relates to the application of arginine in improving monacolin K production by Monascus purple, belonging to the field of biotechnology preparation. Background technique [0002] Monascus, also known as "Monascus", belongs to the phylum Fungi, Ascomycota, Ascomycetes, Monascus family, and the genus Monascus in fungal taxonomy. Monascus can produce a variety of secondary metabolites, such as monascus pigment, monacolin K, γ-aminobutyric acid and citrinin, many of which are widely used in food pigments, medicine, wine making, etc., monacolin K, also known as "lovastatin", is a physiologically active polyketide produced by Monascus. Studies have found that monacolin K can inhibit the activity of the key enzyme HMG-CoA in cholesterol synthesis, and can effectively inhibit cholesterol synthesis. Therefore, monacolin K is regarded as an ideal drug for lowering cholesterol at home and abroad. [0003] The low content of active ingredients of mo...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12N1/38C12R1/645
CPCC12N1/38C12P17/06
Inventor 张婵王成涛孙宝国朱倩倩张函李颖慧
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY