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Microalgae seed conservation technology by freeze-drying and dehydration method

A dehydration and drying technology, which is applied in the field of microalgae seed preservation by freeze-drying dehydration method, can solve the problems of short seed preservation time, small microalgae individuals, difficult separation, etc., and achieve simple preparation method, long seed preservation time and simple operation Effect

Inactive Publication Date: 2019-12-31
邓敏丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Microalgae are rich in nutrients and have a wide range of applications in people's lives and production practices. In the cultivation and application of microalgae, species preservation technology is very important. The large-scale microalgae industry is not enough to cultivate microalgae alone. The algae species must be preserved for later use to meet the needs, but the individual microalgae are small and difficult to separate, and it is easy for the single-cell pure species of the algae species to mutate with environmental changes during the species preservation process, losing their own physiological activity and genetic characteristics, so The preservation of algae species is particularly important. At present, the method of preserving algae species often adopts the method of subculture, which is repetitive and cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A technology for preserving microalgae species by freeze-drying and dehydration method, in order to prepare microalgae species preservation, it is carried out according to the following steps:

[0014] (A1) Algae cultivation: First, culture the algae liquid and experimental materials in a petri dish under aseptic operation for several days, then collect the algae by centrifugation, add fresh medium and resuspend to the required density, and set aside;

[0015] (A2) Filtration of algae species: Pour an appropriate amount of fresh algae species suspension into a filter cloth with a mesh size of 25 μm, filter several times, and wash with twice distilled water to obtain algae mud;

[0016] (A3) Seed preservation by freeze-drying and dehydration method: put the algae mud into the -4 ℃ refrigerator for a period of time, take it out and put it in the freeze dryer, set the program of the freeze dryer, take it out after 3 days of freeze-drying, and store it in liquid nitrogen.

Embodiment 2

[0018] A technology for preserving microalgae species by freeze-drying and dehydration method, in order to prepare microalgae species preservation, it is carried out according to the following steps:

[0019] (A1) Algae cultivation: First, culture the algae liquid and experimental materials in a petri dish under aseptic operation for several days, then collect the algae by centrifugation, add fresh medium and resuspend to the required density, and set aside;

[0020] (A2) Filtration of algae species: Pour an appropriate amount of fresh algae species suspension into a filter cloth with a mesh size of 10 μm, filter several times, and wash with twice distilled water to obtain algae mud;

[0021] (A3) Seed preservation by freeze-drying and dehydration method: put the algae mud into the -4°C refrigerator for a period of time, take it out and put it in the freeze dryer, set the program of the freeze dryer, take it out after 4 days of freeze-drying, and store it in liquid nitrogen.

Embodiment 3

[0023] A technology for preserving microalgae species by freeze-drying and dehydration method, in order to prepare microalgae species preservation, it is carried out according to the following steps:

[0024] (A1) Algae cultivation: First, culture the algae liquid and experimental materials in a petri dish under aseptic operation for several days, then collect the algae by centrifugation, add fresh medium and resuspend to the required density, and set aside;

[0025] (A2) Filtration of algae species: Pour an appropriate amount of fresh algae species suspension into a filter cloth with a mesh size of 50 μm, filter several times, and wash with twice distilled water to obtain algae mud;

[0026] (A3) Seed preservation by freeze-drying and dehydration method: put the algae mud into the -4°C refrigerator for a period of time, take it out and put it in the freeze dryer, set the program of the freeze dryer, take it out after 5 days of freeze-drying, and store it in liquid nitrogen.

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Abstract

The invention provides microalgae seed conservation technology by a freeze-drying and dehydration method in the technical field of microalgae seed conservation. An algae liquid and the experimental materials are first cultured in a petri dish under aseptic operation for several days, then the algae is harvested by centrifugation, a fresh medium is added and re-suspension is carried out to a required density for standby application, an appropriate amount of a microalgae suspension solution is weighed, filtration is carried out by filtration cloth with a certain mesh, washing with distilled water is carried out for two times, an algal slurry is put into a -4 DEG C refrigerator for a period of pre-freezing, and then taken out and put into a lyophilizer, a lyophilizer program is set, taking out is performed after lyophilization, and cold storage in liquid nitrogen is carried out. The method is simple in preparation method, simple in operation, and not easy to pollute, and has a relativelylong seed conservation time.

Description

[0001] technology neighborhood [0002] The invention relates to a seed preservation technology, in particular to a microalgae seed preservation technology by freeze-drying and dehydration method. Background technique [0003] Microalgae are rich in nutrients and have a wide range of applications in people's lives and production practices. In the cultivation and application of microalgae, species preservation technology is very important. The large-scale microalgae industry is not enough to cultivate microalgae alone. The algae species must be preserved for later use to meet the needs, but the individual microalgae are small and difficult to separate, and it is easy for the single-cell pure species of the algae species to mutate with environmental changes during the species preservation process, losing their own physiological activity and genetic characteristics, so Preservation of algae species is particularly important. At present, the method of preserving algae species ofte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/12C12Q1/04
CPCC12N1/04C12N1/12
Inventor 邓敏丽
Owner 邓敏丽