Drugs for inhibiting whitefly acquisition and transmission of geminiviruses and methods for controlling transmission of geminiviruses
A technology of geminivirus and whitefly, which is applied in the field of inhibiting the acquisition of whitefly, disseminating geminivirus, and controlling the spread of geminivirus, so as to ensure the healthy development and hinder the spread of spread
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Embodiment 1
[0029] Effects of feeding anti-CUBN antibody on the ability of Bemisia tabaci to acquire and transmit the virus.
[0030] 1. Feeding anti-CUBN antibody affects the efficiency of geminivirus acquisition by Bemisia tabaci in the early stage of virus acquisition
[0031] (1) Dissolve the anti-CUBN antibody (Product No. ab191073; Abcam; 0.712mg / ml) in 15% sucrose solution at a dilution ratio of 1:50. ml) with ddH 2 O was diluted to 0.712 mg / ml and dissolved in 15% sucrose water at a ratio of 1:50.
[0032] (2) Take a cylindrical double-pass glass tube with an inner diameter of 2.4cm, an outer diameter of 2.7cm, and a height of 10cm, pull the Parafilm membrane to the end of the double-pass glass tube, and drop 250 μl of sucrose solution dissolved with anti-CUBN antibody At the same time, the sucrose solution dissolved in rabbit pre-immune serum was used as a negative control, and then the second layer of Paraflim was applied to make the solution evenly cover the entire glass tube...
Embodiment 2
[0064] Effects of dsAMN injection on the ability of Bemisia tabaci to acquire and transmit the virus.
[0065] 1. Injection of dsAMN affects the efficiency of acquiring geminivirus in Bemisia tabaci in the early stage of virus acquisition
[0066] (1) Design specific primers for the AMN gene, and use cDNA as a template to amplify double-stranded DNA (dsAMN for short), whose sequence information is shown in SEQ ID NO.1 (5'→3');
[0067] (2) using the amplified double-stranded DNA as a template to synthesize double-stranded RNA by in vitro transcription;
[0068] (3) purification by phenol-chloroform extraction to obtain double-stranded RNA;
[0069] (4) Using a capillary, microinject double-stranded RNA into MEAM1 Bemisia tabaci, using dsGFP (sequence shown in SEQ ID NO.2) as a negative control;
[0070] (5) After 48 hours, the treatment group and control group Bemisia tabaci were transferred to tomatoes infected with tomato yellow leaf curl virus (TYLCV) to obtain the virus ...
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