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Preparation method of antioxidant broad-spectrum bacteriostatic agent

A bacteriostatic agent and anti-oxidation technology, applied in the field of anti-oxidative broad-spectrum bacteriostatic agent and its preparation, can solve problems such as lack of anti-oxidative properties, achieve good anti-bacterial properties and anti-oxidative ability, broaden anti-bacterial spectrum, and prepare Simple process effect

Inactive Publication Date: 2020-01-10
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the application of antioxidants and bacteriostatic agents in food is to play their respective functions. For example, lysozyme is often used in food preservation and antiseptic work, but it lacks antioxidant properties (Wang Wang, Guan Rongfa, Liu Zhenfeng, etc., Food Science, 2018, 39 (11):227-232; Xu, Jin, Zhang, et al., Food Research International, 2014, 59:61-66.)

Method used

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  • Preparation method of antioxidant broad-spectrum bacteriostatic agent
  • Preparation method of antioxidant broad-spectrum bacteriostatic agent
  • Preparation method of antioxidant broad-spectrum bacteriostatic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of preparation method of antioxidant broad-spectrum antibacterial agent, comprises the following steps:

[0027] Step 1: Dissolve 1 g of lysozyme (Ly) in 100 ml of ultrapure water in a round-bottomed flask, stir at 400 rpm for 2 hours, add 1 ml of hydrogen peroxide with a concentration of 10 mol / L and 0.25 g of ascorbic acid, finally put into a water bath at 25°C and stir (400 rpm) for 2 hours to obtain mixed solution A;

[0028] Step 2: Add tea polyphenols (EGCG) to the mixed solution A obtained in step 1, and stir at 400 rpm for 24 hours to obtain a mixed solution B. The concentration of tea polyphenols in the mixed solution B is 0.1mmol / L; then the mixed solution B is placed in a water bath at 25°C and continues to stir (400 rpm) for 24 hours;

[0029] Step 3: dialyze the solution obtained in step 2, and dialyze a 3600Da dialysis bag at 25°C for 48 hours to obtain a Ly-EGCG composite system;

[0030] Step 4: The Ly-EGCG composite system obtained from the dia...

Embodiment 2

[0033] A kind of preparation method of antioxidant broad-spectrum antibacterial agent, comprises the following steps:

[0034] Step 1: Dissolve 2g of lysozyme in 200ml of ultrapure water in a round bottom flask, stir at 400 rpm for 2 hours, add 2ml of hydrogen peroxide and 0.5g of ascorbic acid with a concentration of 10mol / L to the Ly solution , and finally put it into a water bath (500 rpm) and stir at 25°C for 2 hours to obtain a mixed solution A;

[0035] Step 2: Add EGCG to the mixed solution A obtained in step 1, stir at 400 rpm for 24 hours to obtain a mixed solution B, the concentration of tea polyphenols in the mixed solution B is 0.3mmol / L; then put the mixed solution B into a water bath Continue stirring (500 rpm) at 25°C for 24 hours;

[0036] Step 3: dialyze the solution obtained in step 2, and dialyze a 3600Da dialysis bag at a temperature of 25°C for 48 hours to obtain a Ly-EGCG composite system;

[0037] Step 4: The Ly-EGCG composite system obtained from the ...

Embodiment 3

[0040] A kind of preparation method of antioxidant broad-spectrum antibacterial agent, comprises the following steps:

[0041] Step 1: Dissolve 1g of lysozyme in 100ml of ultrapure water in a round bottom flask, stir at 400 rpm for 2 hours, add 1ml of hydrogen peroxide and 0.25g of ascorbic acid with a concentration of 10mol / L to the Ly solution , and finally placed in a water bath at 25°C and stirred (400 rpm) for 2 hours to obtain a mixed solution A;

[0042] Step 2: Add EGCG to the mixed solution A obtained in step 1, stir at 400 rpm for 24 hours to obtain a mixed solution B, the concentration of tea polyphenols in the mixed solution B is 0.5mmol / L; then put the mixed solution B into a water bath Continue to stir (400 rpm) at 25°C for 24 hours;

[0043] Step 3: dialyze the solution obtained in step 2, and dialyze a 3600Da dialysis bag at a temperature of 25°C for 48 hours to obtain a Ly-EGCG composite system;

[0044] Step 4: The Ly-EGCG composite system obtained from the...

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Abstract

The invention provides an antioxidant broad-spectrum bacteriostatic agent and a preparation method thereof. The bacteriostatic agent is a lysozyme-tea polyphenol copolymer obtained by coupling lysozyme and tea polyphenol by taking ascorbic acid / hydrogen peroxide as a reaction system. The preparation method comprises the following steps: 1, dissolving lysozyme in ultrapure water, adding hydrogen peroxide and ascorbic acid under the protection of nitrogen, and stirring the material in a water bath kettle to obtain a mixed solution A; 2, adding a tea polyphenol solution into the mixed solution A,stirring to obtain a mixed solution B, and then stirring the mixed solution B; 3, dialyzing the solution obtained in the step 2 to obtain a lysozyme-tea polyphenol composite system; and 4, placing the lysozyme-tea polyphenol composite system obtained in the step 3 in a freeze dryer to be subjected to freeze drying to obtain the lysozyme-tea polyphenol copolymer which is the antioxidant broad-spectrum bacteriostatic agent. According to the method, ascorbic acid serves as a reducing agent, hydrogen peroxide serves as an oxidizing agent, toxic and harmful reagents do not need to be added in thepreparation process, and the preparation process is simple, mild and green.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to an antioxidant broad-spectrum antibacterial agent and a preparation method thereof. Background technique [0002] Lysozyme is an alkaline enzyme that can hydrolyze mucopolysaccharides in pathogenic bacteria, mainly by destroying the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, making the cell wall Insoluble mucopolysaccharides are decomposed into soluble glycopeptides, resulting in the rupture of the cell wall and the release of the contents to lyse the bacteria. Lysozyme can also directly combine with negatively charged viral proteins, and form double salts with DNA, RNA, and apoproteins to inactivate the virus. Lysozyme has strong heat resistance. It will not be inactivated by the treatment of organic solvents. When it is transferred to an aqueous solution, the activity of lysozyme can be fully restored. It c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472A23L3/3571
CPCA23L3/3472A23L3/3571
Inventor 许威郑树青李志帆罗登林葛佩佩金伟平孙玉青徐路阳
Owner XINYANG NORMAL UNIVERSITY
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