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A positive quality control solution for annexin V apoptosis detection and preparation method thereof

A technology of positive quality control and formula, which is applied in the field of positive quality control solution for Annexin V apoptosis detection and its preparation, can solve the problems of difficult compensation adjustment, poor repeatability, and low cell ratio, and achieve convenient compensation adjustment and simple operation , good repeatability

Active Publication Date: 2021-12-10
杭州联科生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the compensation adjustment is performed using the positive samples induced by apoptosis in the experiment, it may be difficult or inaccurate to adjust the compensation due to the low proportion of cells positive for apoptosis
In addition, most of the current apoptosis experiments are induced by the addition of apoptosis drugs or the overexpression or knockout of some genes, but this induction method is affected by various factors such as induction concentration and induction time, resulting in different experimental requirements. The repeated conditions are explored many times, so the repeatability of the method is poor

Method used

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  • A positive quality control solution for annexin V apoptosis detection and preparation method thereof
  • A positive quality control solution for annexin V apoptosis detection and preparation method thereof
  • A positive quality control solution for annexin V apoptosis detection and preparation method thereof

Examples

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Effect test

Embodiment 1

[0052] A positive quality control solution for Annexin V apoptosis detection, comprising the following components and formula:

[0053] Sodium perchlorate, monohydrate, final concentration: 0.15M;

[0054] Trisodium citrate, dihydrate, final concentration: 0.05M;

[0055] Magnesium chloride, hexahydrate, final concentration: 0.05M;

[0056] Calcium chloride, dihydrate, final concentration: 0.05M;

[0057] Glycerin, final concentration: 10%;

[0058] Isobutanol, final concentration: 0.92%;

[0059] Formaldehyde, final concentration: 4.04%;

[0060] Its preparation method is: accurately weigh sodium perchlorate, monohydrate, trisodium citrate, dihydrate, magnesium chloride, hexahydrate and calcium chloride, dihydrate into a beaker, add ultrapure water, stir and mix well, fully dissolve Accurately measure glycerin, isobutanol and formaldehyde, add to the above solution, stir and mix well, and put it in a fume hood overnight; adjust the pH value of the solution to 7 with hydr...

Embodiment 2

[0069] A positive quality control solution for Annexin V apoptosis detection, comprising the following components and formula:

[0070] Sodium perchlorate, monohydrate, final concentration: 0.1M;

[0071] Trisodium citrate, dihydrate, final concentration: 0.075M;

[0072] Magnesium chloride, hexahydrate, final concentration: 0.075M;

[0073] Calcium chloride, dihydrate, final concentration: 0.075M;

[0074] Glycerin, final concentration: 5%;

[0075] Isobutanol, final concentration: 0.5%;

[0076] Formaldehyde, final concentration: 1%;

[0077] Its preparation method is: accurately weigh sodium perchlorate, monohydrate, trisodium citrate, dihydrate, magnesium chloride, hexahydrate and calcium chloride, dihydrate into a beaker, add ultrapure water, stir and mix well, fully dissolve Accurately measure glycerin, isobutanol and formaldehyde, add to the above solution, stir and mix well, and put it in a fume hood overnight; adjust the pH value of the solution to 7 with hydroch...

Embodiment 3

[0086] A positive quality control solution for Annexin V apoptosis detection, comprising the following components and formula:

[0087] Sodium perchlorate, monohydrate, final concentration: 0.2M;

[0088] Trisodium citrate, dihydrate, final concentration: 0.1M;

[0089] Magnesium chloride, hexahydrate, final concentration: 0.1M;

[0090] Calcium chloride, dihydrate, final concentration: 0.1M;

[0091] Glycerin, final concentration: 15%;

[0092] Isobutanol, final concentration: 1%;

[0093] Formaldehyde, final concentration: 4%;

[0094] Its preparation method is: accurately weigh sodium perchlorate, monohydrate, trisodium citrate, dihydrate, magnesium chloride, hexahydrate and calcium chloride, dihydrate into a beaker, add ultrapure water, stir and mix well, fully dissolve Accurately measure glycerin, isobutanol and formaldehyde, add to the above solution, stir and mix well, and put it in a fume hood overnight; adjust the pH value of the solution to 7 with hydrochloric a...

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Abstract

The invention discloses a positive quality control solution for Annexin V apoptosis detection and a preparation method thereof, which is applied to Annexin V or PI / 7-AAD single staining, and an appropriate amount of positive quality control solution is added to normally collected cells, and the mixture is placed on ice After incubation for 30 minutes, the cells can be killed, and then mixed with the same amount of living cells to form a close proportion of living cells and dead cells. When Annexin V or PI / 7‑AAD is single-stained, a close proportion of positive cells can be detected The population and the negative cell population can be very conveniently used for the compensation adjustment of the instrument. The operation is simple, the repeatability is good, the compensation adjustment is convenient, and the reliability of the result is guaranteed.

Description

technical field [0001] The invention relates to a positive quality control solution for Annexin V apoptosis detection and a preparation method thereof. Background technique [0002] Apoptosis, also known as programmed cell death (PCD), is the autonomous and orderly death of cells controlled by genes, and is an active, programmed, and inherent biological process of cells. Apoptosis is an important part of the cell life cycle, and the process of apoptosis is accompanied by the change of cell morphology and the activation, expression and regulation of a series of genes. Among them, phosphatidylserine (PS) in the cell membrane is turned over from the inner layer of the cell membrane to the outer layer, and then exposed to the cell surface, which is the initial event of the apoptosis cascade reaction. The eversion of PS is a sign that apoptotic cells are recognized by phagocytes, which further causes cell shrinkage, chromatin condensation, and nuclear DNA degradation. Therefore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14G01N1/30
CPCG01N15/1404G01N1/30G01N2015/1006G01N2001/302G01N15/01
Inventor 郭爱龙
Owner 杭州联科生物技术股份有限公司