Unlock instant, AI-driven research and patent intelligence for your innovation.

Positive quality control liquid for Annexin V apoptosis detection and preparation method thereof

A technology of positive quality control and formula, which is applied in the field of positive quality control solution for Annexin V apoptosis detection and its preparation, can solve the problems of difficult compensation adjustment, low cell ratio, poor repeatability, etc., and achieve convenient compensation adjustment and repeatability Good, simple operation effect

Active Publication Date: 2020-01-10
杭州联科生物技术股份有限公司
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the compensation adjustment is performed using the positive samples induced by apoptosis in the experiment, it may be difficult or inaccurate to adjust the compensation due to the low proportion of cells positive for apoptosis
In addition, most of the current apoptosis experiments are induced by the addition of apoptosis drugs or the overexpression or knockout of some genes, but this induction method is affected by various factors such as induction concentration and induction time, resulting in different experimental requirements. The repeated conditions are explored many times, so the repeatability of the method is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Positive quality control liquid for Annexin V apoptosis detection and preparation method thereof
  • Positive quality control liquid for Annexin V apoptosis detection and preparation method thereof
  • Positive quality control liquid for Annexin V apoptosis detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A positive quality control solution for Annexin V apoptosis detection, comprising the following components and formula:

[0053] Sodium perchlorate, monohydrate, final concentration: 0.15M;

[0054] Trisodium citrate, dihydrate, final concentration: 0.05M;

[0055] Magnesium chloride, hexahydrate, final concentration: 0.05M;

[0056] Calcium chloride, dihydrate, final concentration: 0.05M;

[0057] Glycerin, final concentration: 10%;

[0058] Isobutanol, final concentration: 0.92%;

[0059] Formaldehyde, final concentration: 4.04%;

[0060] Its preparation method is: accurately weigh sodium perchlorate, monohydrate, trisodium citrate, dihydrate, magnesium chloride, hexahydrate and calcium chloride, dihydrate into a beaker, add ultrapure water, stir and mix well, fully dissolve Accurately measure glycerin, isobutanol and formaldehyde, add to the above solution, stir and mix well, and put it in a fume hood overnight; adjust the pH value of the solution to 7 with hydr...

Embodiment 2

[0069] A positive quality control solution for Annexin V apoptosis detection, comprising the following components and formula:

[0070] Sodium perchlorate, monohydrate, final concentration: 0.1M;

[0071] Trisodium citrate, dihydrate, final concentration: 0.075M;

[0072] Magnesium chloride, hexahydrate, final concentration: 0.075M;

[0073] Calcium chloride, dihydrate, final concentration: 0.075M;

[0074] Glycerin, final concentration: 5%;

[0075] Isobutanol, final concentration: 0.5%;

[0076] Formaldehyde, final concentration: 1%;

[0077] Its preparation method is: accurately weigh sodium perchlorate, monohydrate, trisodium citrate, dihydrate, magnesium chloride, hexahydrate and calcium chloride, dihydrate into a beaker, add ultrapure water, stir and mix well, fully dissolve ; Accurately measure glycerin, isobutanol and formaldehyde, add to the above solution, stir and mix well, and put it in a fume hood overnight; adjust the pH value of the solution to 7 with hydro...

Embodiment 3

[0086] A positive quality control solution for Annexin V apoptosis detection, comprising the following components and formula:

[0087] Sodium perchlorate, monohydrate, final concentration: 0.2M;

[0088] Trisodium citrate, dihydrate, final concentration: 0.1M;

[0089] Magnesium chloride, hexahydrate, final concentration: 0.1M;

[0090] Calcium chloride, dihydrate, final concentration: 0.1M;

[0091] Glycerin, final concentration: 15%;

[0092] Isobutanol, final concentration: 1%;

[0093] Formaldehyde, final concentration: 4%;

[0094] Its preparation method is: accurately weigh sodium perchlorate, monohydrate, trisodium citrate, dihydrate, magnesium chloride, hexahydrate and calcium chloride, dihydrate into a beaker, add ultrapure water, stir and mix well, fully dissolve ; Accurately measure glycerin, isobutanol and formaldehyde, add to the above solution, stir and mix well, and put it in a fume hood overnight; adjust the pH value of the solution to 7 with hydrochloric...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a positive quality control liquid for Annexin V apoptosis detection and a preparation method thereof. The positive quality control liquid for Annexin V apoptosis detection is applied to Annexin V or PI / 7-AAD single staining, a proper amount of positive quality control liquid is added into the normally collected cells, after the cells are incubated on ice for 30 minutes, thecells can die and then are mixed with the same amount of living cells to form living cells and dead cells with approximate proportions, and positive cell populations and negative cell populations with approximate proportions can be detected during Annexin V or PI / 7-AAD single staining, so that the positive quality control liquid for Annexin V apoptosis detection can be very conveniently used forcompensation adjustment of instruments. The operation is simple, the repeatability is good, the compensation adjustment is convenient, and the result reliability is ensured.

Description

technical field [0001] The invention relates to a positive quality control solution for Annexin V apoptosis detection and a preparation method thereof. Background technique [0002] Apoptosis, also known as programmed cell death (PCD), is the autonomous and orderly death of cells controlled by genes, and is an active, programmed, and inherent biological process of cells. Apoptosis is an important part of the cell life cycle, and the process of apoptosis is accompanied by the change of cell morphology and the activation, expression and regulation of a series of genes. Among them, phosphatidylserine (PS) in the cell membrane is turned over from the inner layer of the cell membrane to the outer layer, and then exposed to the cell surface, which is the initial event of the apoptosis cascade reaction. The eversion of PS is a sign that apoptotic cells are recognized by phagocytes, which further causes cell shrinkage, chromatin condensation, and nuclear DNA degradation. Therefore...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N1/30
CPCG01N15/1404G01N1/30G01N2015/1006G01N2001/302G01N15/01
Inventor 郭爱龙
Owner 杭州联科生物技术股份有限公司