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Cryptoporus Volvatus fruiting body polysaccharide and applications in treatment of gout by inhibiting xanthine oxidase activity

A cryptoporous fungus seed and entity technology, applied in the field of chemistry, can solve the problems such as polysaccharide gout that are not disclosed in the cryptoporous fungus fruiting body

Inactive Publication Date: 2020-01-14
南京温博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is currently no published research on the use of Cryptoporus sporocarp polysaccharides for inhibiting xanthine oxidase activity in the treatment of gout

Method used

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  • Cryptoporus Volvatus fruiting body polysaccharide and applications in treatment of gout by inhibiting xanthine oxidase activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Preparation of polysaccharide from the fruiting body of Cryptoporus sporocarpus

[0019] Take 1.5kg of dried Cryptoporus fruiting body powder, add 25L of distilled water, heat and extract at 85°C (2h×3 times), combine the extracts, centrifuge to get the supernatant, concentrate under reduced pressure to 3L, add 3 times the volume of absolute ethanol, 4 Stand at ℃ overnight (12h), centrifuge (12000r / min×15min), collect the precipitate, wash with 75% ethanol, absolute ethanol, acetone successively, redissolve with distilled water, deproteinize by Sevag method, freeze-dry to obtain crude polysaccharide.

[0020] Take the crude polysaccharide, redissolve it with distilled water, prepare a 10 mg / mL crude polysaccharide solution, load the sample on a DEAE-Sepharose Fast Flow chromatography column (3.5 cm inner diameter, 30 cm column bed height), and successively wash with distilled water (12 column volumes) and 1mol / L NaCl solution (10 column volumes) for elutio...

Embodiment 2

[0021] Example 2: In vitro anti-xanthine oxidase activity of polysaccharides from the fruiting body of Cryptoporus sp.

[0022] The in vitro anti-xanthine oxidase activity was improved with reference to the method published by Professor Jiang Jianqin of China Pharmaceutical University (Research on the inhibition of xanthine oxidase activity and its mechanism by chemical components in Daphne radix, Chinese Journal of Experimental Formulas, March 2014), according to the method It can be seen that uric acid is in the range of 0.001~0.1mmol / L and A 290nm have a good linear relationship.

[0023] 1. Test materials

[0024] The fruiting body polysaccharide of Cryptopore sporocarpus was prepared according to the method in Example 1, and stored in a dry place at 4°C.

[0025] K H 2 PO 4 、K 2 HPO 4 ·3H 2 O and DMSO were analytically pure, xanthine was purchased from Sigma, and allopurinol was purchased from Shanghai Zhenzhun Biotechnology Co., Ltd., with a purity of not less tha...

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Abstract

The invention discloses Cryptoporus Volvatus fruiting body polysaccharide and applications in treatment of gout by inhibiting xanthine oxidase activity. According to the invention, the Cryptoporus Volvatus fruiting body polysaccharide has high inhibition activity on xanthine oxidase; the research results in the prior art show that hyperuricemia and gout are caused by too high uric acid in body fluid, uric acid is mainly divided into exogenous uric acid and endogenous uric acid, the exogenous uric acid is mainly taken in through food, the endogenous uric acid is mainly generated by catalyzing xanthine and hypoxanthine through XOD, the excessive uric acid can be generated when the XOD in a body is increased, and the concentration of uric acid is too high to cause hyperuricemia so as to causegout. According to the invention, the Cryptoporus Volvatus fruiting body polysaccharide can be developed into anti-gout drugs.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a cryptoporus fruiting body polysaccharide and its use for inhibiting xanthine oxidase activity and treating gout. Background technique [0002] Xanthine oxidase (Xanthine oxidase, XOD) plays an important role in nucleic acid catabolism. It can catalyze the production of uric acid from xanthine and hypoxanthine. Stress is an important factor leading to gout. Gout is a common joint inflammatory disease, which is a metabolic inflammatory response disease caused by the presence of uric acid in the form of salt in joints and cartilage tissues. Hyperuricemia and gout are both caused by excessive uric acid in body fluids. Uric acid is mainly divided into exogenous and endogenous. Exogenous uric acid is mainly ingested through food, and endogenous uric acid is mainly catalyzed by XOD. Produced by xanthine, when the XOD in the body increases, excessive uric acid will be produced. E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P19/06
CPCA61K31/715A61P19/06C08B37/00C08B37/0003
Inventor 不公告发明人
Owner 南京温博生物科技有限公司