Application of Clostridium autoethanogenum protein in freshwater carnivorous fish feeds
A technology of Clostridium ethanol and fish feed, which is applied in the field of feed, can solve the problems of increased feed coefficient, high fish meal price, and reduced growth performance, and achieve the effects of reducing feed cost, reducing the amount of fish meal, and being easy to digest and absorb
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[0023] This embodiment provides an application of Clostridium ethanol protein in freshwater carnivorous fish feed. In the application, the preparation method of Clostridium ethanol protein includes:
[0024] Clostridium autoethanogenum (Clostridium autoethanogenum) is used as a fermentation strain, and CO-containing gas is used as a raw material to carry out liquid fermentation culture, and the bacteria in the fermentation liquid of the liquid fermentation are centrifuged and dried once to obtain Clostridium autoethanogenum protein;
[0025] In this example, the Clostridium autoethanogenum (Clostridium autoethanogenum) preservation number is DSM10061, and the preservation address is German Culture Collection, DSMZ;
[0026] In the liquid fermentation culture, the concentration of Clostridium ethanol is 20-25g / L, and the amount of CO gas introduced is 8000-10000m 3 / h;
[0027] The centrifugal speed is 4000-4500r / min, and the centrifugal feed flow rate is 4-6m 3 / h; the bacte...
Embodiment 1
[0041]In parts by weight, the feed formula for freshwater carnivorous fish is as follows: 4.5 parts of clostridium ethanol protein, 35 parts of fish meal, 12 parts of cassava flour, 3 parts of gluten powder, 14.8 parts of cottonseed protein concentrate, 14.8 parts of soybean protein concentrate, blood cell powder 3 parts, 1 part of calcium dihydrogen phosphate, 0.4 part of choline chloride, 8.5 parts of oil, 1.5 parts of sodium hydroxymethyl cellulose, 1 part of premix, 0.5 part of microcrystalline cellulose.
[0042] The preparation method of freshwater carnivorous fish feed is as follows: add the clostridium ethanol protein to the mixture passing through the 80 mesh sieve, add water, mix well, and use the F-26 (II) type granulator to make a particle size of 2.0mm Granular, dried at 65°C to obtain feed for freshwater carnivorous fish.
Embodiment 2
[0044] This embodiment differs from Embodiment 1 in that:
[0045] In parts by weight, the feed formula for freshwater carnivorous fish is as follows: 9 parts of clostridium ethanol protein, 30 parts of fish meal, 12 parts of cassava flour, 3 parts of gluten powder, 14.8 parts of cottonseed protein concentrate, 14.8 parts of soybean protein concentrate, blood cell powder 3 parts, 1 part of calcium dihydrogen phosphate, 0.4 part of choline chloride, 8.9 parts of oil, 1.5 parts of sodium polyacrylate, 1 part of premix, 0.6 part of microcrystalline cellulose.
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