Application of Clostridium autoethanogenum protein in freshwater carnivorous fish feeds

Abstract

The invention relates to application of Clostridium autoethanogenum protein in freshwater carnivorous fish feeds, and belongs to the field of feeds. As for the application of the Clostridium autoethanogenum protein in the freshwater carnivorous fish feeds provided by the invention, a preparation method of the Clostridium autoethanogenum protein comprises the following steps: taking Clostridium autoethanogenum as a fermentative bacterium as well as gas containing CO as a raw material so as to carry out liquid-state fermenting-culturing to obtain fermentation broth; and then, centrifuging thallus in the fermentation broth, and carrying out primary drying so as to obtain the Clostridium autoethanogenum protein. The application of the Clostridium autoethanogenum protein in the freshwater carnivorous fish feeds provided by the embodiments of the invention has the following advantages: 1, being used as an alternative for fish meal, the Clostridium autoethanogenum protein is free of adverse effects on fish body, so that the Clostridium autoethanogenum protein can be used as one of high-quality protein source substitutes for the fish meal; and 2, compared with low-temperature dried fish meal, which is good in quality as well as good in digestion and absorption rates yet relatively high in price, the Clostridium autoethanogenum protein is capable of reducing cost by 5.58-16.73% on the basis of acting as a good substitution for the fish meal. Therefore, obvious economic benefits are ensured.

Description

technical field

[0001] The invention belongs to the field of feed, and in particular relates to the application of clostridium ethanol protein in freshwater carnivorous fish feed. Background technique

[0002] Largemouth bass (Micropterus salmoides), commonly known as California perch and black perch, belongs to Perciformes, Porcoidei, Cehtrachidae, and Micropterus, native to North America. Taiwan Province of my country introduced this fish from abroad in the late 1970s, and succeeded in artificial breeding in 1983. It was introduced into Guangdong Province in the same year. It is currently one of the important freshwater carnivorous fish breeding species in China. Largemouth bass has the advantages of strong adaptability, fast growth, easy catch, and short breeding cycle. In addition, the meat is delicious and tender, without intermuscular spines, and has a beautiful appearance. It is very popular among farmers and consumers. Fishmeal is an indispensable protein raw materia...

Examples

preparation example Construction

[0023] This embodiment provides an application of Clostridium ethanol protein in freshwater carnivorous fish feed. In the application, the preparation method of Clostridium ethanol protein includes:

[0024] Clostridium autoethanogenum (Clostridium autoethanogenum) is used as a fermentation strain, and CO-containing gas is used as a raw material to carry out liquid fermentation culture, and the bacteria in the fermentation liquid of the liquid fermentation are centrifuged and dried once to obtain Clostridium autoethanogenum protein;

[0025] In this example, the Clostridium autoethanogenum (Clostridium autoethanogenum) preservation number is DSM10061, and the preservation address is German Culture Collection, DSMZ;

[0026] In the liquid fermentation culture, the concentration of Clostridium ethanol is 20-25g / L, and the amount of CO gas introduced is 8000-10000m 3 / h;

[0027] The centrifugal speed is 4000-4500r / min, and the centrifugal feed flow rate is 4-6m 3 / h; the bacte...

Embodiment 1

[0041]In parts by weight, the feed formula for freshwater carnivorous fish is as follows: 4.5 parts of clostridium ethanol protein, 35 parts of fish meal, 12 parts of cassava flour, 3 parts of gluten powder, 14.8 parts of cottonseed protein concentrate, 14.8 parts of soybean protein concentrate, blood cell powder 3 parts, 1 part of calcium dihydrogen phosphate, 0.4 part of choline chloride, 8.5 parts of oil, 1.5 parts of sodium hydroxymethyl cellulose, 1 part of premix, 0.5 part of microcrystalline cellulose.

[0042] The preparation method of freshwater carnivorous fish feed is as follows: add the clostridium ethanol protein to the mixture passing through the 80 mesh sieve, add water, mix well, and use the F-26 (II) type granulator to make a particle size of 2.0mm Granular, dried at 65°C to obtain feed for freshwater carnivorous fish.

Embodiment 2

[0044] This embodiment differs from Embodiment 1 in that:

[0045] In parts by weight, the feed formula for freshwater carnivorous fish is as follows: 9 parts of clostridium ethanol protein, 30 parts of fish meal, 12 parts of cassava flour, 3 parts of gluten powder, 14.8 parts of cottonseed protein concentrate, 14.8 parts of soybean protein concentrate, blood cell powder 3 parts, 1 part of calcium dihydrogen phosphate, 0.4 part of choline chloride, 8.9 parts of oil, 1.5 parts of sodium polyacrylate, 1 part of premix, 0.6 part of microcrystalline cellulose.

technical field

[0001] The invention belongs to the field of feed, and in particular relates to the application of clostridium ethanol protein in freshwater carnivorous fish feed. Background technique

[0002] Largemouth bass (Micropterus salmoides), commonly known as California perch and black perch, belongs to Perciformes, Porcoidei, Cehtrachidae, and Micropterus, native to North America. Taiwan Province of my country introduced this fish from abroad in the late 1970s, and succeeded in artificial breeding in 1983. It was introduced into Guangdong Province in the same year. It is currently one of the important freshwater carnivorous fish breeding species in China. Largemouth bass has the advantages of strong adaptability, fast growth, easy catch, and short breeding cycle. In addition, the meat is delicious and tender, without intermuscular spines, and has a beautiful appearance. It is very popular among farmers and consumers. Fishmeal is an indispensable protein raw materia...

Examples

preparation example Construction

[0023] This embodiment provides an application of Clostridium ethanol protein in freshwater carnivorous fish feed. In the application, the preparation method of Clostridium ethanol protein includes:

[0024] Clostridium autoethanogenum (Clostridium autoethanogenum) is used as a fermentation strain, and CO-containing gas is used as a raw material to carry out liquid fermentation culture, and the bacteria in the fermentation liquid of the liquid fermentation are centrifuged and dried once to obtain Clostridium autoethanogenum protein;

[0025] In this example, the Clostridium autoethanogenum (Clostridium autoethanogenum) preservation number is DSM10061, and the preservation address is German Culture Collection, DSMZ;

[0026] In the liquid fermentation culture, the concentration of Clostridium ethanol is 20-25g / L, and the amount of CO gas introduced is 8000-10000m 3 / h;

[0027] The centrifugal speed is 4000-4500r / min, and the centrifugal feed flow rate is 4-6m 3 / h; the bacte...

Embodiment 1

[0041]In parts by weight, the feed formula for freshwater carnivorous fish is as follows: 4.5 parts of clostridium ethanol protein, 35 parts of fish meal, 12 parts of cassava flour, 3 parts of gluten powder, 14.8 parts of cottonseed protein concentrate, 14.8 parts of soybean protein concentrate, blood cell powder 3 parts, 1 part of calcium dihydrogen phosphate, 0.4 part of choline chloride, 8.5 parts of oil, 1.5 parts of sodium hydroxymethyl cellulose, 1 part of premix, 0.5 part of microcrystalline cellulose.

[0042] The preparation method of freshwater carnivorous fish feed is as follows: add the clostridium ethanol protein to the mixture passing through the 80 mesh sieve, add water, mix well, and use the F-26 (II) type granulator to make a particle size of 2.0mm Granular, dried at 65°C to obtain feed for freshwater carnivorous fish.

Embodiment 2

[0044] This embodiment differs from Embodiment 1 in that:

[0045] In parts by weight, the feed formula for freshwater carnivorous fish is as follows: 9 parts of clostridium ethanol protein, 30 parts of fish meal, 12 parts of cassava flour, 3 parts of gluten powder, 14.8 parts of cottonseed protein concentrate, 14.8 parts of soybean protein concentrate, blood cell powder 3 parts, 1 part of calcium dihydrogen phosphate, 0.4 part of choline chloride, 8.9 parts of oil, 1.5 parts of sodium polyacrylate, 1 part of premix, 0.6 part of microcrystalline cellulose.

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