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Product and application thereof to preservation of sea cucumbers

A product, the sea cucumber technology, is applied in chemical preservation of meat/fish, protein-containing food ingredients, food science, etc. It can solve the problems of fresh and live sea cucumbers transported to inland areas, and the impact of fresh and live sea cucumbers on transportation and storage, so as to achieve inhibition Autolysis phenomenon, effect of prolonging storage time

Pending Publication Date: 2020-01-21
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This phenomenon has a great impact on the transportation and storage of fresh and live sea cucumbers, making it difficult to transport fresh and live sea cucumbers to inland areas

Method used

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  • Product and application thereof to preservation of sea cucumbers
  • Product and application thereof to preservation of sea cucumbers
  • Product and application thereof to preservation of sea cucumbers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Inhibitor preparation: Z-DEVD-FMK and 3-MA were dissolved in DMSO respectively, the initial concentration of Z-DEVD-FMK was 5 mM, and the initial concentration of 3-MA was 1 mM. Dilute Z-DEVD-FMK and 3-MA with purified water, mix the diluted Z-DEVD-FMK and 3-MA, and finally add soluble cellulose powder to obtain the inhibitor. Among the inhibitors, the concentration of Z-DEVD-FMK was 20 μM, that of 3-MA was 5 μM, and that of soluble cellulose was 5 mg / mL.

[0056] (2), the experimental process:

[0057] Fresh and live imitation sea cucumbers with equal size and intact body wall were used, 5 each in the normal group, Z-DEVED-FMK group and Z-DEVD-FMK+3-MA+cellulose group.

[0058] The sea cucumbers in the normal group did not receive any treatment.

[0059] In the Z-DEVE-FMK group, Z-DEVD-FMK solution was injected at a concentration of 20 μM, and injected into sea cucumbers at a rate of 2 mL / only from the tail cloaca.

[0060] The Z-DEVD-FMK+3-MA+cellulose group wa...

Embodiment 2

[0065] (1) Inhibitor preparation: Z-DEVD-FMK and 3-MA were dissolved in DMSO respectively, Z-DEVD-FMK was prepared at a concentration of 10 mM, and 3-MA at a concentration of 2 mM. Then dilute Z-DEVD-FMK and 3-MA with low-temperature seawater, mix the diluted Z-DEVD-FMK and 3-MA, and finally add soluble cellulose powder to obtain the inhibitor. Among the inhibitors, the concentration of Z-DEVD-FMK was 15 μM, that of 3-MA was 10 μM, and that of soluble cellulose was 5 mg / mL.

[0066] (2), the implementation process:

[0067] Fresh Prunus ginseng with equal size and intact body wall was used, 5 in each of the normal group, Z-DEVED-FMK group and Z-DEVD-FMK+3-MA+cellulose group.

[0068] The sea cucumbers in the normal group did not receive any treatment.

[0069] The Z-DEVE-FMK group was injected with Z-DEVD-FMK solution at a concentration of 15 μM, and injected into the sea cucumber body by inserting 2 mL / only from the tail cloaca orifice.

[0070] The Z-DEVD-FMK+3-MA+cellulo...

Embodiment 3

[0075] (1) Inhibitor preparation: Z-DEVD-FMK was dissolved in DMSO at a concentration of 10 mM, and diluted to 30 μM with purified water.

[0076] (2) Experimental process: fresh green sea cucumbers of equal size and intact body wall were used, 5 in each of the normal group and the Z-DEVD-FMK group. The sea cucumbers in the Z-DEVD-FMK group were injected with the solution prepared by the aforementioned Z-DEVD-FMK, and injected into the sea cucumbers by inserting 1 mL / sea cucumber from the tail cloaca orifice. The normal group was not treated. Afterwards, the two groups of sea cucumbers were drained of seawater and placed in black packaging bags filled with 1L of low-temperature seawater (4°C). The mouth of the bag was fastened, filled with 20mL of oxygen, and placed in a foam box at 4°C. Take pictures to monitor each group of changes, and record its status.

[0077] (3) Experimental results: The experimental results show that compared with the untreated control group, Z-DEVD...

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Abstract

The invention relates to a product and application thereof to the preservation of sea cucumbers. The product includes one or two selected from polypeptide Z-DEVD-FMK and derivatives of the polypeptideand 6-amidogen-3-methyl purines. The product can significantly inhibit autolysis of the sea cucumbers, and prolongs the preservation time of the fresh and alive sea cucumbers.

Description

technical field [0001] The application generally relates to the fields of food and medicine, and specifically relates to a product and its application in sea cucumber preservation. Background technique [0002] Sea cucumbers, belonging to the class Holothuroidea, are marine echinoderms that live by the sea to a depth of 8,000 meters, and are widely distributed in oceans all over the world. There are many kinds of sea cucumbers along the coast of the South my country Sea, and there are about 20 kinds of sea cucumbers available for consumption. Sea cucumber is a rare seafood with very high nutritional and economic value. However, sea cucumbers are prone to autolysis due to changes in environmental factors such as temperature, salinity, radiation, light, and physical entity contact after being salvaged from the water. Autolysis is manifested as a peeling phenomenon, that is, white plaques appear on the body wall and fester, and finally the whole body melts into a viscous past...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B4/20
CPCA23B4/20A23V2002/00A23V2250/55
Inventor 杨静峰张美迪邢洁王艳杰孙景赫
Owner DALIAN POLYTECHNIC UNIVERSITY
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