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Pichia pastoris containing milk cow feed and production method thereof

A technology of Pichia pastoris and dairy cow feed, which is applied in the field of cow feed containing Pichia pastoris and its production, can solve the problems of polluting the environment, wasting protein resources, etc., achieve energy saving, inhibit yeast autolysis, and improve the body's disease resistance Effect

Active Publication Date: 2014-03-26
JINAN BEST ANIMAL HUSBANDRY TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above deficiencies in the utilization of discarded Pichia pastoris, many discarded Pichia pastoris are thrown away for nothing, which not only wastes protein resources, but also pollutes the environment

Method used

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  • Pichia pastoris containing milk cow feed and production method thereof
  • Pichia pastoris containing milk cow feed and production method thereof
  • Pichia pastoris containing milk cow feed and production method thereof

Examples

Experimental program
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Effect test

example 1

[0024] 1.1 Experimental animals and feeding

[0025] CMT reagent was used to detect recessive mastitis in a dairy farm in Jiyang County, and 24 dairy cows with similar parity, lactation period, milk production and positive recessive mastitis were selected as experimental animals and randomly divided into control group and experimental group. Groups, 12 pigs in each group, were fed the control diet and the experimental diet, respectively. The pre-feeding period was 15 days, and the test period was 45 days, a total of 50 days.

[0026] 1.2 Diet preparation

[0027] Test diet formulation:

[0028] According to CuSO 4 .5H 2 O0.9%, FeSO 4 .7H 2 O3.2%, ZnSO 4 .7H 2 O4.8%, MnSO 4 .1H 2 O1.8%, mass percent concentration is 1% KI premix 0.18%, mass percent concentration is 1% Na 2 SeO 3 0.20% premix, 1% CoCl by mass 2 Premix 0.09%, Vitamin A 0.24%, Vitamin D 3 0.13%, 1.4% vitamin E, 1.22% niacin and 85.84% zeolite powder are mixed evenly to make a premix;

[0029] Accord...

example 2

[0050] 2.1.1 Experimental animals and feeding

[0051] The experiment was carried out on a dairy farm in Shanghe County. A total of 96 black-and-white dairy cows with similar parity, lactation month and daily lactation volume were selected and randomly divided into 2 groups, with 4 replicates in each group and 12 cows in each replicate. The control group was fed the control diet, and the experimental group was fed the experimental diet. The test officially started after 1 week of pre-feeding, and the formal test period was 56 days. The control group and the test group were fed twice a day, depending on the amount left in the trough. Milk 3 times a day. The daily milk production of each cow was counted every day. Other feeding and management were as usual, and the conditions of the two groups were the same. At the end of the experiment, 8 jugular veins were randomly selected in each group to collect blood, and blood antioxidant indexes were measured.

[0052] 2.1.2 Diet p...

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Abstract

The invention discloses a pichia pastoris containing milk cow feed, belonging to the technical field of animal husbandry. The milk cow feed is prepared through mixing a pichia pastoris concentrate for a milk cow and a roughage feed; the pichia pastoris concentrate for a milk cow is composed of a wet yeast feed and dry concentrate powder; the wet yeast feed is composed of waste pichia pastoris, baking soda and water; and grape seeds, jujube powder and honeysuckle are added in the dry concentrate powder. According to the invention, the processing and application modes of the waste pichia pastoris are changed, the waste pichia pastoris is compatible with health-care products, and then the wet feed is fed, therefore, the yeast autolysis is restrained, the activities of saccharomycetes and enzymes thereof are kept, the energy is saved, active saccharomycetes and enzyme preparations of milk cows are supplemented, the body disease resistance is improved, the occurrence of bovine mastitis is reduced, and the utilization of non-conventional feed raw materials is promoted.

Description

technical field [0001] The invention relates to a feed production method, in particular to a dairy cow feed containing Pichia pastoris and a production method thereof. Background technique [0002] As a single-cell protein, yeast is rich in nutrients, and the protein content of the bacteria is as high as 40-80%. The amino acid components are complete, and the content of essential amino acids such as lysine is relatively high. It is also rich in vitamins and nucleotides. As a yeast, Pichia pastoris is also a carrier for the expression of active substances such as proteins and enzymes. As an expression vector, Pichia pastoris has attracted more attention. At present, many enzyme products, such as cellulase, phytase, xylanase, etc., are highly expressed in Pichia pastoris. While expressing enzyme preparations, Pichia pastoris will produce a large number of cells. If these yeast cells are not treated, they will not only waste resources but also seriously pollute the environmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/18A23K1/16A23K1/14A23K1/175
CPCY02P60/87
Inventor 杨赵军杨林青盛清凯姚强刘华阳李明晖宣玉娟
Owner JINAN BEST ANIMAL HUSBANDRY TECH
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