Method for extracting Yunzhi polysaccharopeptide with high-quality
An extraction method, the technology of Yunzhi intracellular glycopeptide, applied in the field of biological extraction, can solve the problems of high free peptide content, affecting the overall quality, and no extraction method of Yunzhi glycopeptide products, so as to achieve high content of glycopeptide polymers , the effect of less free peptide content
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] Example 1: Take 450 g of Yunzhi mycelium powder (20190510) that has been dried and crushed to pass through a 60-mesh sieve, add 15 times the weight of purified water, put it in a round-bottomed flask, and boil it at 100°C for 3 hours in an electric heating mantle. The extract was centrifuged at 4000r / min for 10min to obtain 4500ml of supernatant. The supernatant was divided into 4 parts, namely 300ml, 600ml, 1200ml and 2400ml. , 1.04, 1.09, 1.18 (measured at room temperature). Slowly add ethanol with a mass concentration of 95%, and stir rapidly. When the flocs are filled with the solution, stir again for 1 min, so that the final ethanol concentration reaches 70%, stand at 4°C overnight, and centrifuge at 4000 r / min for 5 min. The precipitate was collected and dried in an oven at 75°C. The results are shown in Table 1
[0034] Table 1 The effect of concentrated liquid density on intracellular glycopeptides of Versicolor versicolor
[0035] Concentrate densi...
Embodiment 2
[0036] Example 2: Take 1000g of Yunzhi mycelium powder (batch number 20190607) that has been dried and crushed to pass through a 60-mesh sieve, add 15 times the weight of purified water, put it in a round-bottomed flask and boil it at 100°C for 3 hours in an electric heating mantle to obtain The extract was centrifuged at 4000r / min for 10min to obtain 5890ml of supernatant, which was divided into 6 parts, namely 190ml, 380ml, 760ml, 1140ml, 1520ml and 1900ml, and the 6 parts of supernatant were all concentrated into relative densities Respectively 0.988, 0.991, 1.028, 1.066, 1.094, 1.119 (measured at room temperature). Slowly add ethanol with a mass concentration of 95%, and stir rapidly. When the flocs are filled with the solution, stir again for 1 min to make the final ethanol concentration reach 70%. Let stand overnight at 4°C, collect the precipitate, and put it in an oven at 75°C for drying. The results are shown in Table 2:
[0037] Table 2 The effect of concentrated li...
Embodiment 3
[0039] Example 3: Weigh 1000 g of Yunzhi mycelium powder (batch number 20190819) that has been dried and crushed to pass through a 60-mesh sieve, add 10 times the weight of purified water, place it in a round-bottomed flask, and boil it at 100°C for 3 hours on an electric heating mantle. The obtained extract was centrifuged at 4000r / min for 5min to obtain 7750ml of supernatant, which was concentrated to obtain 1800ml of concentrated solution. The relative density of the solution was 1.04 as measured at normal temperature. Divide the concentrated solution into 9 parts, each 200ml, slowly add 95% ethanol, stir quickly, when the flocs are full of solution, stir again for 1min, so that the concentration of ethanol in the concentrated solution reaches 20%, 30%, 40%, 50% respectively , 55%, 60%, 65%, 70%, 80%. Place in a refrigerator at 4°C for alcohol precipitation overnight. The concentrated liquid was centrifuged at 4000r / min for 5min to obtain a precipitate, and the precipitate...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap