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Method for extracting Yunzhi polysaccharopeptide with high-quality

An extraction method, the technology of Yunzhi intracellular glycopeptide, applied in the field of biological extraction, can solve the problems of high free peptide content, affecting the overall quality, and no extraction method of Yunzhi glycopeptide products, so as to achieve high content of glycopeptide polymers , the effect of less free peptide content

Active Publication Date: 2020-01-21
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large-scale industrial production of Yunzhi intracellular glycopeptides is mostly water extraction, and the Yunzhi glycopeptides are obtained through alcohol precipitation. Even though the total sugar content is high, the content of useless free peptides is too high, which affects the overall quality
At present, there is no research report on the extraction method of Yunzhi glycopeptide products that controls the relationship between total sugar and peptide ratio.

Method used

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  • Method for extracting Yunzhi polysaccharopeptide with high-quality
  • Method for extracting Yunzhi polysaccharopeptide with high-quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Take 450 g of Yunzhi mycelium powder (20190510) that has been dried and crushed to pass through a 60-mesh sieve, add 15 times the weight of purified water, put it in a round-bottomed flask, and boil it at 100°C for 3 hours in an electric heating mantle. The extract was centrifuged at 4000r / min for 10min to obtain 4500ml of supernatant. The supernatant was divided into 4 parts, namely 300ml, 600ml, 1200ml and 2400ml. , 1.04, 1.09, 1.18 (measured at room temperature). Slowly add ethanol with a mass concentration of 95%, and stir rapidly. When the flocs are filled with the solution, stir again for 1 min, so that the final ethanol concentration reaches 70%, stand at 4°C overnight, and centrifuge at 4000 r / min for 5 min. The precipitate was collected and dried in an oven at 75°C. The results are shown in Table 1

[0034] Table 1 The effect of concentrated liquid density on intracellular glycopeptides of Versicolor versicolor

[0035] Concentrate densi...

Embodiment 2

[0036] Example 2: Take 1000g of Yunzhi mycelium powder (batch number 20190607) that has been dried and crushed to pass through a 60-mesh sieve, add 15 times the weight of purified water, put it in a round-bottomed flask and boil it at 100°C for 3 hours in an electric heating mantle to obtain The extract was centrifuged at 4000r / min for 10min to obtain 5890ml of supernatant, which was divided into 6 parts, namely 190ml, 380ml, 760ml, 1140ml, 1520ml and 1900ml, and the 6 parts of supernatant were all concentrated into relative densities Respectively 0.988, 0.991, 1.028, 1.066, 1.094, 1.119 (measured at room temperature). Slowly add ethanol with a mass concentration of 95%, and stir rapidly. When the flocs are filled with the solution, stir again for 1 min to make the final ethanol concentration reach 70%. Let stand overnight at 4°C, collect the precipitate, and put it in an oven at 75°C for drying. The results are shown in Table 2:

[0037] Table 2 The effect of concentrated li...

Embodiment 3

[0039] Example 3: Weigh 1000 g of Yunzhi mycelium powder (batch number 20190819) that has been dried and crushed to pass through a 60-mesh sieve, add 10 times the weight of purified water, place it in a round-bottomed flask, and boil it at 100°C for 3 hours on an electric heating mantle. The obtained extract was centrifuged at 4000r / min for 5min to obtain 7750ml of supernatant, which was concentrated to obtain 1800ml of concentrated solution. The relative density of the solution was 1.04 as measured at normal temperature. Divide the concentrated solution into 9 parts, each 200ml, slowly add 95% ethanol, stir quickly, when the flocs are full of solution, stir again for 1min, so that the concentration of ethanol in the concentrated solution reaches 20%, 30%, 40%, 50% respectively , 55%, 60%, 65%, 70%, 80%. Place in a refrigerator at 4°C for alcohol precipitation overnight. The concentrated liquid was centrifuged at 4000r / min for 5min to obtain a precipitate, and the precipitate...

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Abstract

The invention discloses a method for extracting Yunzhi polysaccharopeptide with high quality. The method comprises the following steps: drying and grinding Yunzhi mycelia; performing boiling and extracting for 2-4 hours to obtain extract having a material-material ratio of 1:5-1:15; centrifuging the extract to obtain supernate; concentrating the supernate, detecting the relative density of the concentrate being 0.98-1.18 at normal temperature; adding alcohol having a concentration of 95 percent until the alcohol concentration is 45-50 percent, performing alcohol precipitation, and performing refrigeration overnight; and performing centrifuging and separating to obtain precipitate, and drying the precipitate. The product prepared by the method has high content of total saccharides and low content of peptides.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and relates to a method for extracting glycopeptides from Yunzhi mycelium, in particular to a method for extracting intracellular glycopeptides from Yunzhi by controlling the total sugar and peptide content. Background technique [0002] Yunzhi intracellular glycopeptide is a high molecular glycopeptide polymer extracted from the mycelia of Polyporaceae plant Yunzhi. It has the function of replenishing essence, invigorating the spleen and nourishing the heart, and it also has a certain protective effect on cellular immune function and blood picture. For patients with esophageal cancer, gastric cancer and primary lung cancer who suffer from deficiency of Qi and Yin, heart and spleen deficiency caused by radiotherapy and chemotherapy. According to the research, it is found that polysaccharides in Yunzhi glycopeptides have various effects, and the peptides that have therapeutic effect...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/30C12N1/14C12R1/645
CPCC07K1/145C07K1/30C12N1/14
Inventor 曹宝臣贾存江袁峰
Owner JIANGSU SHENHUA PHARMA
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