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Saccharopolyspora pogona engineering strain with double cspA gene and application thereof

A technology of saccharopolyspora chinensis and engineering strains, applied in the field of saccharopolyspora chinensis engineering strains, can solve problems such as difficulties, and achieve the effects of reducing production costs and increasing yield

Active Publication Date: 2020-01-21
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently, in Saccharopolyspora CspA There are no reports on the research, and there are great difficulties in transferring foreign genes into S.

Method used

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  • Saccharopolyspora pogona engineering strain with double cspA gene and application thereof
  • Saccharopolyspora pogona engineering strain with double cspA gene and application thereof
  • Saccharopolyspora pogona engineering strain with double cspA gene and application thereof

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Embodiment

[0033] In this embodiment cspA Gene doubled engineered strain of Saccharopolyspora spp. cspA Integrated into the genome of Saccharopolyspora.

[0034] Saccharopolyspora seropolyspora cold shock protein synthesis gene doubling engineered strain of the present invention, that is Saccharopolyspora seropolysporaS. pogona::cspA , was deposited in the China Center for Type Culture Collection (CCTCC for short, address: Wuhan University, Wuhan, China) on March 20, 2019, and the strain preservation number is CCTCC NO: M 2019181.

[0035] Further, the cold shock protein gene cspA , cloned from Saccharopolyspora betabilis, its sequence is as follows:

[0036] atggcacagg gaaccgtgaa gtggttcaac gcggaaagg gcttcggctt tatcgccccc 60

[0037] gacgacggtg gcccggacgt attcgtgcac ttctcggcca tcgaggcctc gggcttccgt 120

[0038] gagctgtccg aggaccagca cgtgacctac gaggtcaccc agggccctaa gggcccgcag 180

[0039] gccgccagcg tgcgcgtctg a 201

[0040] (1) Specific medium formula and culture conditions

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Abstract

The invention relates to a Saccharopolyspora pogona engineering strain with double cspA gene and an application thereof. The Saccharopolyspora pogona engineering strain with double cspA gene is produced by integrating a cold shock protein gene cspA into a chromosome set of Saccharopolyspora pogona. The Saccharopolyspora pogona engineering strain with double cspA gene,namely as Saccharopolyspora pogona S.pogona, is deposited in China Center for Type Culture Collection on March 20, 2019, and the preservation number of the strain is CCTCC NO: M 2019181. Compared with the original strain S.pogona,the biomass of the engineering strain S.pogona:: cspA with double cspA gene of the invention is increased by 1.6 times, and the mycelium morphology is also changed from branched filamentous to shortrod-shaped. The results of shake flask fermentation shows that, the engineering strain S.pogona:: cspA with double cspA gene is applied to the biosynthesis of butenyl spinosad, and the yield of butenyl spinosad is increased by 4 times compared with the biosynthesis of butenyl spinosad by using the original strain S.pogona.

Description

technical field [0001] The present invention relates to a strain of Saccharopolyspora mentabilis gene overexpression engineering strain and its application, especially relates to a strain cspA Gene-doubled engineering strain of Saccharopolyspora mentabilis and its application. Background technique [0002] Agriculture is the foundation of the national economy, and crop pests are the main obstacle to the sustainable development of modern agriculture in our country. The use of chemical pesticides has enhanced the ability of humans to control pests and diseases, and has made a significant contribution to recovering crop yield losses. However, the long-term dependence and extensive use of chemical pesticides have caused a series of problems such as serious environmental pollution, destruction of ecological balance, and food safety. Biopesticides are more and more concerned and favored in the current pesticide market because of their advantages such as strong selectivity, no ha...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/74C12P19/62C12R1/01
CPCC07K14/195C12N15/74C12P19/62
Inventor 夏立秋穰杰何昊城丁学知孙运军
Owner HUNAN NORMAL UNIVERSITY
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