Device and method for hatching shrimp fertilized eggs in vitro

Abstract

The invention relates to a device and method for hatching shrimp fertilized eggs in vitro. The method comprises the following steps: stripping fertilized egg blocks from the abdomen of a parent shrimp; placing the separated fertilized egg blocks on a sieve of 20-40 meshes, then placing the fertilized egg blocks into a mixed solution of protease and carbohydrase for shaking enzymolysis, and inflating the mixed solution, so that the fertilized egg blocks are cracked to form a fertilized egg monomer or a single chain; then sterilizing, rinsing twice in sterilized seawater, and hatching the fertilized egg monomer by adopting a shrimp fertilized egg in-vitro hatching device: uniformly laying the fertilized egg monomer subjected to enzymolysis, disinfection and cleaning on a silk tray, and thenplacing the fertilized egg monomer in a culture container; and carrying out aeration on the water in a water storage container so that the dissolved oxygen amount is increased to form a high-dissolvedoxygen solution, continuously pumping the high-dissolved oxygen water into the culture container by a water pump, controlling the daily water exchange amount in the culture container to be about 1 / 3so that the fertilized eggs are in a gentle high-dissolved oxygen water flow and develop into shrimp larvae, and the larvae are collected through a shrimp culture lure lamp.

Description

technical field [0001] The invention relates to an in vitro hatching device and hatching method for fertilized shrimp eggs. Background technique [0002] Precious shrimps have high requirements on the environment. In the process of artificial fishing or cultivation, shrimps are prone to death, especially after the death of broodstock with eggs, it is necessary to separate the fertilized eggs as soon as possible and then put them in a suitable The hatching culture is carried out in a sterile water body with water temperature; the existing technology usually adopts aeration to the hatching tank to ensure that the dissolved oxygen in the hatching tank meets the training requirements, however, the fertilized eggs are easily damaged or ruptured by the impact of air bubbles during the aeration process, And in order to ensure the quality of the water body, the existing technology needs to frequently replace a large amount of water in the hatching tank, which consumes a lot of water...

AI Technical Summary

Problems solved by technology

[0002] Precious shrimps have high requirements on the environment. In the process of artificial fishing or cultivation, shrimps are prone to death, especially after the death of broodstock with eggs, it is necessary to separate the fertilized eggs as soon as possible and then put them in a suitable The hatching culture is carried out in a sterile water body with water temperature; the existing technology usually adopts aeration to the hatching tank to ensure that the dissolved oxygen in the hatching tank meets the training requirements, however, the fertilized eggs are easily damaged or ruptured by the impact of air bubbles during the aeration process, And in order to ensure the quality of the water body, the existing technology needs to frequently replace a large amount of water in the hatching tank, which consumes a lot of water and is likely to cause sudden changes in the microenvironment of the water body

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