A reagent vibration mixing method
A reagent and mixing technology, applied in the field of biochip detection, can solve problems such as unfavorable working life of vibration motors, and achieve the effects of reducing voltage, improving detection throughput, and reducing processing accuracy requirements.
Active Publication Date: 2020-04-10
HUNAN LEGEND AI CHIP BIOTECH CO LTD
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And the working voltage applied to the vibrating motor when mixing (the voltage is generally 3.5V-4.5V when starting) is also higher than the rated voltage (the rated voltage is generally 3V), which is not good for the working life of the vibrating motor
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[0039] The present invention will be described in detail below with reference to the accompanying drawings and examples. It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other. For the convenience of description, if the words "up", "down", "left" and "right" appear in the following, it only means that the directions of up, down, left and right are consistent with the drawings themselves, and do not limit the structure.
[0040] A method for vibrating and mixing reagents, in which dropping reagents and vibrating and mixing are carried out synchronously. Compared with the situation in the prior art where adding reagents and vibrating and mixing are carried out separately, the time consumption of the present invention is obviously shortened, which is conducive to shortening the detection time , improve detection efficiency.
[0041] Under a given vibration amplitude, the le...
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The invention discloses a reagent vibration mixing method. The reagent vibration mixing method comprises the following steps: adding a first part of reagent into a reactor filled with solid particles, and at the same time turning on a vibration motor at the bottom of the reactor to place the solid particles Mix with the first part of the reagent; stop adding the first part of the reagent, and continue to vibrate for 0s-10s to form a suspension; gradually add the second part of the reagent and vibrate at the same time until mixed; wherein, the amount of the second part of the reagent added is the first part of the reagent 5-10 times of the amount; the power supply voltage of the vibration motor is the same constant voltage V. The invented mixing method can not only achieve the effect of rapid and stable mixing, but also reduce the requirements on the processing accuracy and consistency of related parts.
Description
technical field [0001] The invention relates to a reagent vibration mixing method, which belongs to the field of biochip detection, and is especially suitable for liquid-liquid or solid-liquid mixing. Background technique [0002] For the disposable microfluidic biochip developed by the applicant, the reagents are quantitatively added to the reactor for reaction by dropping, and the reactor is an open local area inside the chip, so it cannot be stirred by stirring. Realize the mixing of liquid reagents in the reactor. The liquid volume in the reactor is generally small (less than 0.5 ml) and it is an open container. If ultrasonic mixing is used, atomization problems will inevitably occur, and there is a potential risk of cross-contamination. At the same time, since the reactor is installed inside the chip, if the vortex is used for mixing, the whole chip needs to participate in the vibration together, which will lead to a relatively large volume of the mixing structure, and...
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IPC IPC(8): B01F11/00B01F15/02B01L3/00
CPCB01L3/5027B01F31/60B01F31/70B01F35/3204B01F35/712B01F2101/23
Inventor 邬鹏程黄常青
Owner HUNAN LEGEND AI CHIP BIOTECH CO LTD