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A sample split pipeline of a microfluidic chip

A microfluidic chip and shunt tube technology, which is applied in the field of biological protein analysis, can solve the problems of the large amount of samples consumed, the inability to guarantee the sufficient amount of samples in each quantitative centrifuge chamber, and the effect of reducing the supply amount.

Active Publication Date: 2021-11-19
BEIJING 3S CENTURY TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a sample shunt pipeline of a microfluidic chip, which solves the problem that in the prior art, sufficient samples cannot be guaranteed for each quantitative centrifuge chamber, or more samples need to be provided, thereby Causes the problem of large sample size consumed

Method used

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  • A sample split pipeline of a microfluidic chip
  • A sample split pipeline of a microfluidic chip
  • A sample split pipeline of a microfluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment one, such as figure 1 with figure 2 As shown, a sample distribution pipeline of a microfluidic chip includes a first liquid storage end, a plurality of second liquid storage ends 2, and a split channel 3 corresponding to the second liquid storage end 2 one-to-one, and the split channel 3 It communicates with the second liquid storage end 2, and the second liquid storage end 2 is far away from the rotation center of the microfluidic chip relative to the distribution channel 3, and several distribution channels 3 are connected end to end, and the distribution channel 3 includes : a buffer unit 31, the buffer unit 31 communicates with the inlet of the second liquid storage end 2; a liquid inlet 32, the liquid inlet 32 ​​communicates with the first liquid storage end and the buffer unit 31; and, A liquid outlet 33 , the liquid outlet 33 is arranged on the end of the buffer unit 31 away from the connection between the buffer unit 31 and the inlet of the second l...

Embodiment 2

[0031] Embodiment two, such as image 3 As shown, on the basis of Embodiment 1, the sample distribution pipeline also includes a sample outlet flow channel 5, through which the adjacent split flow channels 3 are connected, specifically, one end of the sample outlet flow channel 5 is connected to the upper The liquid outlet 33 of one distribution tank 3 is connected to the liquid inlet 32 ​​of the next distribution tank 3 at the other end, so that the communication between adjacent distribution tanks 3 is realized.

[0032] The sample splitting pipeline provided by Embodiment 2 of the present invention, when in use, with the rotation of the microfluidic chip, the excess sample in the previous splitting tank 3 overflows from the liquid outlet 33 and flows to the next one through the sample outlet channel 5 The liquid inlet end 32 of the splitter tank 3 enters the next splitter tank 3. Similarly, the sample of the buffer unit 31 of the next splitter tank 3 is further thrown into ...

Embodiment 3

[0033] Embodiment three, such as Figure 4 to Figure 6 As shown, on the basis of Embodiment 2, the sample shunting pipeline also includes a sample chamber 6, which is close to the center of rotation of the microfluidic chip relative to the shunt groove 3, and the sample chamber 6 and the first shunt groove 3 are filled with liquid. The first liquid storage end corresponding to end 32 is connected.

[0034] The sample distribution pipeline also includes a residual liquid collection chamber 7, which is arranged at the end of the sample distribution pipeline away from the sample chamber 6, and the residual liquid collection chamber 7 communicates with the liquid outlet 33 of the final distribution tank 3, In the third embodiment, the residual liquid collection chamber 7 is preferably set at the end of the sample distribution pipeline away from the sample chamber 6 .

[0035]When in use, with the rotation of the microfluidic chip, due to the effect of centrifugal force, the sampl...

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Abstract

The invention discloses a sample shunt pipeline of a microfluidic chip, comprising a first liquid storage end, a plurality of second liquid storage ends, and shunt grooves corresponding to the second liquid storage ends one by one, the shunt groove and the second liquid storage end Connected, the second liquid storage end is far away from the rotation center of the microfluidic chip relative to the splitter tank, and several splitter slots are connected end to end, and the splitter tank includes: a buffer unit, which communicates with the inlet of the second liquid storage end; The end is connected to the first liquid storage end and the buffer unit; and, the liquid outlet end is set on the end of the buffer unit away from the connection between the buffer unit and the inlet of the second liquid storage end; the centrifugal chamber is sequentially added by using the splitter groove For samples, only after the previous centrifuge chamber is fully filled, the redundant sample will fill the next centrifuge chamber, so as to ensure that each quantitative centrifuge chamber has enough samples, and at the same time, compared with the existing technology, the overall sample supply is reduced.

Description

technical field [0001] The invention belongs to the field of biological protein analysis, and in particular relates to a sample distribution pipeline of a microfluidic chip. Background technique [0002] Microfluidic chip refers to the integration of basic operating units such as sample preparation, reaction, separation, and detection involved in the fields of chemistry and biology into a small chip, and a network of microchannels is formed, with controllable fluid running through the entire chip. System, used to realize various functions of conventional chemical or biological laboratory. At present, in order to improve the accuracy of detection, the detection reagents of microfluidic chips are usually liquid detection reagents preloaded in a certain proportion. After the sample to be detected and the liquid detection reagent are mixed in the reaction chamber and a sufficient immune reaction is carried out, different types of optical analyzers are used to detect the liquid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/50273B01L2200/10B01L2300/0861B01L2400/0409
Inventor 彭勇冯尧
Owner BEIJING 3S CENTURY TECH CORP