Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of culture medium and method for inhibiting browning of exotic walnut explants

A technique for exotic walnuts and explants, applied in the field of medium for inhibiting browning of exotic walnut explants, can solve problems such as culture failure, death, and growth inhibition of explants, and achieve browning control and inhibition of explants Effects of Browning, Anti-browning Rate and Induction Rate Improvement

Active Publication Date: 2021-12-31
TARIM UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of in vitro culture, such substances diffuse into the medium, causing the growth of explants to be inhibited, resulting in death, resulting in culture failure

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The medium for inhibiting browning of walnut explants in this example is DKW basic medium supplemented with 15 mg / L activated carbon, 18 mg / L malic acid, 30 g / L sucrose and 6 g / L agar, with a pH value of 5.7.

[0022] The method for suppressing the browning of the exotic walnut explant of the present embodiment comprises:

[0023] Step 1, select the explant and carry out pretreatment: take the axillary bud stem section of the exotic walnut as the explant; the pretreatment specifically includes the following steps:

[0024] S1. Cleaning: After cleaning the surface dirt with an aqueous solution of detergent, rinse with running water;

[0025] S2. Refrigerate and protect from light at 3°C ​​for 6 hours;

[0026] S3, move to the ultra-clean workbench, use 0.1% v / v HgC1 2 Soaking, soaking seeds and buds with 75% v / v alcohol for 30s, 20% Na 2 S 2 o 3 Soak for 1 min, rinse with sterile water for 8 to 10 times, and dry the surface moisture of the explants with sterile filt...

Embodiment 2

[0029] The medium for inhibiting browning of walnut explants in this example is DKW basic medium supplemented with 10 mg / L activated carbon, 20 mg / L malic acid, 30 g / L sucrose and 6 g / L agar, with a pH value of 5.5.

[0030] The method for suppressing the browning of the exotic walnut explant of the present embodiment comprises:

[0031] Step 1, select the explant and carry out pretreatment: take the axillary bud stem section of the exotic walnut as the explant; the pretreatment specifically includes the following steps:

[0032] S1. Cleaning: After cleaning the surface dirt with an aqueous solution of detergent, rinse with running water;

[0033] S2. Refrigerate and protect from light at 2°C for 4 hours;

[0034] S3, move to the ultra-clean workbench, use 0.1% v / v HgC1 2 Soaking, soaking seeds and buds with 75% v / v alcohol for 30s, 20% Na 2 S 2 o 3 Soak for 1 min, rinse with sterile water for 8 to 10 times, and dry the surface moisture of the explants with sterile filter...

Embodiment 3

[0037] The medium for inhibiting browning of walnut explants in this example is DKW basic medium supplemented with 20 mg / L activated carbon, 15 mg / L malic acid, 30 g / L sucrose and 6 g / L agar, with a pH value of 6.0.

[0038] The method for suppressing the browning of the exotic walnut explant of the present embodiment comprises:

[0039] Step 1, select the explant and carry out pretreatment: take the axillary bud stem section of the exotic walnut as the explant; the pretreatment specifically includes the following steps:

[0040] S1. Cleaning: After cleaning the surface dirt with an aqueous solution of detergent, rinse with running water;

[0041] S2. Refrigerate and protect from light at 4°C for 8 hours;

[0042] S3, move to the ultra-clean workbench, use 0.1% v / v HgC1 2 Soaking, soaking seeds and buds with 75% v / v alcohol for 30s, 20% Na 2 S 2 o 3 Soak for 1 min, rinse with sterile water for 8 to 10 times, and dry the surface moisture of the explants with sterile filter...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a kind of culture medium and method for suppressing the browning of exotic walnut explants, and described culture medium comprises basal medium, activated carbon and malic acid; Said method comprises: (1) selecting explant and carrying out pretreatment (2) Inoculate the pretreated explants in the medium for inhibiting the browning of the explants of the miraculous walnut and cultivate them under dark conditions at 12°C to 14°C for a period of time, then alternately cultivate them in light and dark under cultivation. The culture medium and method for inhibiting the browning of explants of exotic walnuts provided by the present invention greatly improve the anti-browning rate and induction rate, the anti-browning rate is about 89.92% to 95.18%, and the induction rate is about 45.27% to 53.62%. .

Description

technical field [0001] The invention relates to the technical field of asexual propagation of seedlings, in particular to a medium and a method for inhibiting the browning of explants of exotic walnuts. Background technique [0002] Exotic walnut is an important rootstock used in American walnut production. It is cultivated by natural or artificial hybridization with walnut as the female parent and walnut as the male parent. Using exotic walnuts as walnut rootstocks not only has a strong growth potential, but also resists black thread disease, root neck rot and other diseases, and has greater tolerance to root parasitic nematodes. [0003] Browning and death of explants in plant tissue culture is a major obstacle to the induction of dedifferentiation and regeneration of shoots. Browning is usually due to the oxidation of phenolic substances in plant tissue cells to produce brown quinones, which turn the tissues brown. In the process of in vitro culture, such substances dif...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 金强陈小飞冯一峰陈家力张锐王新建张宏刘多红
Owner TARIM UNIV