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Application of mesenchymal stem cell paracrine factor to preparation of pain drugs

A kind of stem cell and paracrine technology, applied in the field of biological stem cells, can solve the problems that no one has proposed and no one has found anti-pain.

Pending Publication Date: 2020-02-04
北京中广天一生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, mesenchymal stem cells have been successfully used as biopharmaceuticals in the treatment of knee joint diseases, but the main purpose of treatment is to enhance and improve knee joint mobility, or to regenerate knee joint chondrocytes, and to treat pain. no one raised
In addition, the field of mesenchymal stem cells for pain treatment is still blank, and no one has found that it has the function of anti-pain

Method used

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  • Application of mesenchymal stem cell paracrine factor to preparation of pain drugs
  • Application of mesenchymal stem cell paracrine factor to preparation of pain drugs

Examples

Experimental program
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Effect test

preparation example Construction

[0047] Example 1 Preparation of umbilical cord mesenchymal stem cells

[0048] The umbilical cord mesenchymal stem cells described in the present invention are mainly derived from human umbilical cord mesenchymal stem cells, but the source of stem cells is not limited to umbilical cord, and can be derived from multiple human tissue sources such as human placenta, bone marrow, dental pulp, and fat.

[0049] Wherein, the preparation process of umbilical cord mesenchymal stem cells is as follows: see step (1)-step (9) of the summary of the invention;

[0050] Cut the fresh umbilical cord, clean it with sterile normal saline, further remove visible blood vessels and Wahrenheit jelly tissue, cut the umbilical cord to a size of about 1 mm and put it into a cell culture dish, add 10 wt% serum substitute (trade name : Ultroser G serum substitute; product number: 15950-017; purchased from Pall, USA) basal medium (high-glucose DMEM medium) until just covering the tissue block, and cultu...

Embodiment 2

[0053] Example 2 Evaluation experiment of mesenchymal stem cell paracrine factors on stiff neck pain

[0054] Stiff neck is a common disease, which occurs in young adults, more common in winter and spring. The common course of stiff neck is that there is no symptom before going to sleep, but after waking up in the morning, you feel obvious soreness in the nape and back, and the neck movement is limited. Generally, patients with stiff neck need 3-7 days to return to normal. 10 patients with stiff neck were selected, and the local multipoint subcutaneous injection of mesenchymal stem cell paracrine factor was performed on the second day after the occurrence of stiff neck. After the pain assessment, a total of 2ml human umbilical cord mesenchymal For stem cell paracrine factors, the patients were asked to fill out the pain assessment scale on the 2nd, 4th, and 7th day after injection. All 10 patients experienced improvement and relief of pain on the second day after injection, ...

Embodiment 3

[0055] Example 3 Evaluation experiment of mesenchymal stem cells on cervical spondylosis pain

[0056] Chronic pain refers to pain that lasts for more than one month (previously it was three months or half a year). With the advancement of society and the development of computer networks, chronic pain caused by cervical spondylosis is increasing. Eight cases of patients with chronic pain in cervical spondylosis were selected, and the pain of the individuals was evaluated using the Numerical Pain Assessment Scale. After pain assessment, a total of 2 ml of human umbilical cord mesenchymal stem cell paracrine factors were injected locally at multiple points. The patients were asked to fill out the pain assessment scale on the 3rd day, 7th day, 14th day and 30th day after the injection. Eight patients had pain improvement and relief on the second day and two days after injection, and the pain score decreased from 7±3 to 6±2 points, and the pain score continued to be further reduce...

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Abstract

The invention discloses application of a mesenchymal stem cell paracrine factor to preparation of pain drugs, and relates to application of the technical field of biological stem cells to pain. One the one hand, the invention provides a preparation method of the mesenchymal stem cell paracrine factor; on the other hand, it is found that the paracrine factor prepared by the method can be applied tothe treatment of various kinds of pains, the specific pains comprise subjective sensation pains of all patients such as arthralgia, inflammatory pain and cancer pain, and the application prospect ofpreparing pain drugs is achieved. According to the mesenchymal stem cell paracrine factor, the pain caused by various reasons can be effectively alleviated; and a cell protein preparation has excellent biological effect on pain treatment.

Description

technical field [0001] The invention relates to the application of biological stem cell technology in pain, in particular to a preparation method and application of mesenchymal stem cell paracrine factors for treating various pains. Background technique [0002] Pain is an unpleasant sensory and emotional experience caused by tissue damage or potential tissue damage, and is a subjective experience. Pain, together with the four vital signs of human breathing, blood pressure, pulse, and body temperature, is listed as the fifth vital sign. It is known that some physical therapy methods such as massage and infrared can be used, or anti-inflammatory and analgesic drugs can be used to improve and treat pain. Physiotherapy can only temporarily relieve symptoms, while drug analgesia not only produces dependence, but also has relatively large side effects. None of the above methods can improve pain symptoms in the long term. [0003] Mesenchymal stem cells are a type of adult stem ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P29/00C12N5/0775
CPCA61K9/0019A61K35/28A61P29/00C12N5/0668C12N2509/00
Inventor 刘学彬张成城
Owner 北京中广天一生物科技有限公司
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