Application of hydrogen sulfide modified bone marrow mesenchymal stem cells (MSCs) extracellular vesicles (EVs) to hypoxic-ischemic brain damage (HIBD)

A bone marrow mesenchymal, hypoxia-ischemia technology, applied in the field of molecular diagnosis and molecular biology, can solve problems such as unsatisfactory curative effect

Inactive Publication Date: 2020-02-11
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the clinical treatment for this disease is mainly nutritional support and symptomatic treatment, but the curative effect is not satisfactory.

Method used

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  • Application of hydrogen sulfide modified bone marrow mesenchymal stem cells (MSCs) extracellular vesicles (EVs) to hypoxic-ischemic brain damage (HIBD)
  • Application of hydrogen sulfide modified bone marrow mesenchymal stem cells (MSCs) extracellular vesicles (EVs) to hypoxic-ischemic brain damage (HIBD)
  • Application of hydrogen sulfide modified bone marrow mesenchymal stem cells (MSCs) extracellular vesicles (EVs) to hypoxic-ischemic brain damage (HIBD)

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preparation example Construction

[0039] The H 2 The preparation method of S-EVs comprises: adding sodium hydrosulfide (NaHS) into the culture medium of bone marrow mesenchymal stem cells for incubation, and collecting extracellular vesicles secreted by the bone marrow mesenchymal stem cells.

[0040] In yet another specific embodiment of the present invention, a product is provided, the active ingredient of which includes EVs; the product can be used to prevent / or treat hypoxic-ischemic brain damage, and further, has any of the following or multiple uses:

[0041] (a) reducing brain edema induced by hypoxic-ischemic brain damage;

[0042] (b) reducing cerebral infarction induced by hypoxic-ischemic brain damage;

[0043] (c) improving the activation of microglial cells induced by hypoxic-ischemic brain damage;

[0044] (d) inhibiting the expression of pro-inflammatory factors;

[0045] (e) promote the expression of anti-inflammatory factors;

[0046] (f) promotes the polarization of native microglia towa...

Embodiment

[0058] 1. Experimental animals: Whole litters of C57BL / 6J mice on postnatal day 3 (P3) were purchased in batches from the Animal Center of Shandong University. They were raised in the SPF-grade animal breeding room of the Animal Center of Shandong University, with a temperature of 20±2°C and a cycle of 12 hours of light and 12 hours of darkness. Adequate mouse food and water were given during animal feeding, and the animals could freely obtain water and food, so that they could adapt to the environment and grow up to 7 days after birth. Animal experiments were carried out in accordance with the "International Guidelines for Animal Research" provided by the Council of International Organizations of Medical Sciences (CIOMS), and the regulations were strictly followed. The procedures were approved by the Animal Ethics and Welfare Committee of Shandong University.

[0059] 2. Establishment of HIBD model in newborn mice: 7-day-old (P7) C57BL / 6J mice were taken out, and the HIBD mod...

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Abstract

The invention provides application of hydrogen sulfide modified bone marrow mesenchymal stem cells (MSCs) extracellular vesicles (EVs) to hypoxic-ischemic brain damage (HIBD), and belongs to the technical field of molecular diagnosis and molecular biology. Through research, it is found that by adopting H2S pretreated MSCs EVs (H2S-EVs), the therapeutic effect of the H2S-EVs to the HIBD can be effectively improved, specifically, the H2S-EVs can effectively relieve HIBD-induced encephaledema, relieves HIBD-induced cerebral infarction, inhibits expression of proinflammatory factors, promotes expression of anti-inflammatory factors, promotes polarization of originally stationed microglial cells and infiltrated macrophage to the M2 phenotype, and improves the long-term learning and memory functions of HIBD, and thus the H2S-EVs has the good practical application value.

Description

technical field [0001] The invention belongs to the technical fields of molecular diagnosis and molecular biology, and in particular relates to the application of hydrogen sulfide-modified bone marrow mesenchymal stem cell extravesicles in hypoxic-ischemic brain injury. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] With the implementation of the two-child policy, a new production peak has ushered in. However, the high-risk factors of neonatal hypoxic-ischemic encephalopathy (hypoxic-ischemic encephalopathy, HIE) will be an important problem that people will face. HIE refers to hypoxic-ischemic brain damage (HIBD) caused by perinatal asphyxia. It is a common clinical dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K9/10A61K47/46A61P9/10
CPCA61K9/10A61K9/107A61K47/46A61P9/10
Inventor 王贞初锡丽李婷婷辛丹清刘德祥
Owner SHANDONG UNIV
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