Extraction method for dalbergia sissoo and application of extracts in anti-tumor drugs

Abstract

The invention relates to an extraction method for dalbergia sissoo. The dalbergia is leguminosae dalbergia genus plant Dalbergia sissoo Roxb. Extracts contain an alcohol extract, an ethyl ether extract, and a petroleum ether extract. A monomer separated in the extracts is 4-methoxydalbergione. The method specifically includes the following steps: taking dalbergia sissoo heartwood, performing planing to form thin sheets, performing crushing, performing sieving by using a No.2 sieve, and performing reflux extraction under heating for 2-5 times, wherein the extraction solvent is an ethanol or methanol solution, the material-liquid ratio is 1:2 to 1:8, and each time is maintained for 1-3 h; and performing filtration when the solution is hot, combining the filtrates, and performing reduced-pressure concentration to form a thin extract shape. Cell experiments confirm that the dalbergia sissoo has important medicinal value, and the monomer can significantly inhibit the proliferation of humanliver cancer cells HepG2, human breast cancer cells MCF-7, mouse breast cancer cells 4T1 and human glioma cells U87, and affect the morphological functions; and the method provides the basis for the development and utilization of the dalbergia sissoo and provide a novel option for the development of anti-tumor drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the use of the heartwood of Dalbergia indica and its extract in the preparation of antitumor drugs. Background technique [0002] Malignant tumors seriously endanger human health and are serious public health problems that have attracted worldwide attention. The World Health Organization (WHO) data shows that among the population, the number of deaths due to chronic diseases accounts for 70% of all causes of death, among which tumors rank second. According to the forecast of the International Agency for Research on Cancer (IARC), there will be about 17.11 million new cancer cases in the world in 2020, and about 21.65 million people in 2030. The results of the mortality rate of major diseases in my country in 2017 show that the mortality rate of malignant tumors ranks first. It is estimated that the annual death toll of chronic diseases in my country is about 8 milli...

AI Technical Summary

Problems solved by technology

[0006] At present, Indian Dalbergia is not included in the "Chinese Pharmacopoeia", and there are few studies on its pharmacological activity, and its active parts and active ingredient content are not clear enough. The pharmacological activities of the main ingredients are mostly concentrated in flavonoids, and related flavonoids have many specific effects. Such as anti-platelet aggregation, increase coronary flow, anti-inflammatory, antibacterial, anti-oxidation, central nervous system protection of glial cells and anti-tumor effects on osteosarcoma, lung cancer, etc.

[0007] After searching the literature and patents at home and abroad, no reports on the anti-tumor effects of Dalbergia chinensis and its extracts on liver cancer, breast cancer and glioma have been found.

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