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Detection nanoparticle, detection method, kit, and so on, for antigen-specificity T cell content detection

A technology of nanoparticle and cell content, applied in the field of immunoassay, can solve the problem of the scarcity of antigen-specific T cells, and achieve the effects of low cost, avoiding waste, and convenient use

Pending Publication Date: 2020-02-25
SUZHOU ERSHENG BIOPHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, how to effectively detect antigen-specific T cells, especially antigen-specific T cells in peripheral blood, is particularly important. Rare, there is no very effective technical method for detecting the content of some disease-associated antigen-specific T cells

Method used

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  • Detection nanoparticle, detection method, kit, and so on, for antigen-specificity T cell content detection
  • Detection nanoparticle, detection method, kit, and so on, for antigen-specificity T cell content detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Detection of cancer cell antigen-specific T cells in cancer patients

[0052] In this embodiment, the detection of antigen-specific T cells in cancer cells is taken as an example for description.

[0053] Cancer immunotherapy has made great progress in recent years. For example, anti-PD-1 antibody, anti-PD-L1 antibody and CAR-T cell therapy have good anti-cancer effects, and a number of related anti-cancer drugs have obtained American food and drugs. Approved by the Supervision Administration (FDA) and put into market use. The therapeutic mechanism of cancer immunotherapy is to recognize and kill cancer cells through the cancer-related antigen-specific T cells of the own immune system. Because cancer antigen-specific T cells have the characteristics of specificity and memory, once the cancer antigen-specific T cells can recognize and attack cancer cells, the side effects are small and the effect is lasting. However, the overall response rate of current cancer imm...

Embodiment 2

[0115] The following is a description of Embodiment 2. In this embodiment, the detection principle is the same as that of the embodiment 1, and the materials involved in each preparation process and the selection of parameters are based on the same parts, and the same description is omitted.

[0116] In this example, the detection of antigen-specific T cells related to type 1 diabetes is taken as an example.

[0117] Type 1 diabetes is caused by the abnormal recognition and attack of β-cells in the pancreas by β-cell-specific T cells. Once the beta cells are completely killed, the human body will not be able to synthesize and secrete insulin, which can lower blood sugar, and blood sugar will rise. It can only rely on external sources to supplement insulin to maintain blood sugar balance. It can be seen that β-cell-specific T cells play a key role in the occurrence and development of type 1 diabetes.

[0118] For this reason, this example detects the content of β-cell-specific T cel...

Embodiment 3

[0160] The following is a description of Embodiment 3. In this embodiment, the detection principle is the same as that of the embodiment 1, and the materials involved in each preparation process and the selection of parameters are based on the same parts, and the same description is omitted.

[0161] The purpose and significance of this example is the same as that of Example 2. It also takes the detection of antigen-specific T cells related to type 1 diabetes as an example, and is also used to detect β in peripheral blood of patients with insulin-dependent diabetes (type 1 diabetes). The content of cell antigen-specific T cells. The difference between this example and Example 2 is that when preparing the detection nanoparticles, instead of directly encapsulating the antigen related to type 1 diabetes, it encapsulates the genetic material DNA required to synthesize this antigen.

[0162] In this embodiment, the detection nanoparticles are also prepared first, and then the prepared ...

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Abstract

The invention provides a detection particle capable of being effectively used for antigen-specificity T cell content detection, a corresponding preparation method thereof, a kit comprising the detection particle and a detection method for performing antigen-specificity T cell content detection through the detection particle, wherein the detection particle is characterized by being used for activating an antigen-specificity T cell in content detection of the antigen-specificity T cell executed based on any one or more of cellular secretions secreted by the secrete activated antigen-specificityT cell in a to-be-detected sample, proliferation status of the activated antigen-specificity T cell and a cell surface marker of the activated antigen-specificity T cell, and while treating the to-be-detected sample, being capable of being phagocytosed by an antigen presenting cell in the to-be-detected sample to release an antigen or generate the antigen based on a genetic material so as to makethe antigen presenting cell present antigenic epitope of the released or generated antigen to the surface of the antigen presenting cell and enable the corresponding antigen-specificity T cells to recognize and activate the antigen-specificity T cell.

Description

Technical field [0001] The present invention belongs to the field of immunoassay, and specifically relates to a detection nanoparticle that can be effectively used for the detection of antigen-specific T cell content, its corresponding preparation method, a kit including the same, and a method for detecting antigen-specific T cell content using the same. Detection method. Background technique [0002] In recent years, the development of immune technology has been extremely rapid, and breakthroughs have been made in the field of cancer immunotherapy. With the continuous improvement of understanding of various diseases, people have discovered that immune cells play an increasingly important role in the occurrence and treatment of various diseases. [0003] Immune cells include B cells and T cells. T cells play a huge role in both autoimmune response and adaptive immune response. Antigen-specific T cells are one of the main components of the adaptive immune response. Antigen-speci...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54346
Inventor 刘密王镕
Owner SUZHOU ERSHENG BIOPHARMACEUTICAL CO LTD
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