Sample pretreatment solution for quantitative vitamin D detection and use method of solution

Abstract

The invention discloses a sample pretreatment solution for quantitative vitamin D detection. The solution comprises normal saline, dextran sulfate sodium salt and a beta-isotropism blocking agent. Thedextran sulfate sodium salt and the beta-isotropism blocking agent can be used to fully separate 25-hydroxyvitamin D to be analyzed from binding protein to a certain extent. Non-specific interferenceeffect in a serum matrix is reduced, the sample can be well separated in the further separation and purification process, a high-quality to-be-analyzed object can be obtained from the treated sample,and the quantitative detection accuracy of the vitamin D is improved.

Description

technical field

[0001] The invention relates to the field of quantitative detection of vitamin D, in particular to a sample pretreatment solution for quantitative detection of vitamin D and a use method thereof. Background technique

[0002] Vitamin D is a steroid hormone, including 2 types: vitamin D2 and vitamin D3. It is converted into 25-hydroxyvitamin D2 [25-hydroxyvitamin D2, 25 (OH) D2] and 25-hydroxyvitamin D3 [25-hydroxyvitamin D3, 25 (OH) D3] in the body through absorption, collectively referred to as 25-hydroxyvitamin D [ 25-hydroxyvitamin D, 25(OH)D]. 25(OH)D in the systemic circulation of the body is considered to be an important indicator reflecting the level of vitamin D in the human body.

[0003] Vitamin D is an important trace element that participates in the body's physiological functions. The main physiological functions are to participate in the body's calcium and phosphorus metabolism and immune regulation. Patients with osteoporosis, osteomalacia, r...