A method for simultaneously identifying Ganoderma lucidum and Shouwu vine in a Chinese medicine composition
A kind of Shouwu vine, the technology of composition
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Embodiment 1
[0063] Take the traditional Chinese medicine composition, 5g of the negative control substance without the extract of Shouwu Teng, 5g of the negative control without Ganoderma lucidum, 1g of Ganoderma lucidum extract, 1g of Shouwu Teng extract, add 30mL of ethanol, sonicate for 30 minutes, filter , After the filtrate was evaporated to dryness in a water bath, 1 mL of ethanol was added to dissolve it, and they were used as the test solution, negative reference solution and extract solution respectively. In addition, 2 g of Ganoderma lucidum and about 0.25 g of Shouwu Teng reference medicinal materials were taken to make a reference medicinal solution in the same way.
[0064] According to the general rule 0502 test of the 2015 edition of the Pharmacopoeia of the People's Republic of China, draw 5 μL of each of the above solutions, and place them on the same silica gel G thin-layer plate (20cm×10cm, Qingdao Hailang Silica Gel Desiccant Co., Ltd.), and use cyclohexane-ethyl acetat...
Embodiment 2
[0066] Take the traditional Chinese medicine composition, 5g of the negative control substance without the extract of Shouwu Teng, 5g of the negative control without Ganoderma lucidum, 1g of Ganoderma lucidum extract, 1g of Shouwu Teng extract, add 30mL of ethanol, sonicate for 30 minutes, filter , After the filtrate was evaporated to dryness in a water bath, 1 mL of ethanol was added to dissolve it as the test solution, negative reference solution and extract solution. In addition, 2 g of Ganoderma lucidum and about 0.25 g of Shouwu Teng reference medicinal materials were taken to make a reference medicinal solution in the same way.
[0067] According to the "Pharmacopoeia of the People's Republic of China" 2015 edition general rule 0502 test, draw 5 μ L of each of the above solutions, respectively spot on the same silica gel G thin-layer plate (20cm x 10cm, German MACHEREY-NAGEL company), and use cyclohexane-ethyl acetate-formic acid (The volume ratio is 5:5:0.2) as the deve...
Embodiment 3
[0069] Take the traditional Chinese medicine composition, the negative control substance not containing Radix Polygonum extract, the negative control substance not containing Ganoderma lucidum, 5g each, 1g Ganoderma lucidum extract, and 1g Radix Radix Polygonum extract, add 30mL of ethanol, ultrasonicate for 30 minutes, and filter. After the filtrate was evaporated to dryness in a water bath, 1 mL of ethanol was added to dissolve it as the test solution, negative control solution and extract solution. In addition, 2 g of Ganoderma lucidum and about 0.25 g of Shouwu Teng reference medicinal materials were taken to make a reference medicinal solution in the same way.
[0070] According to the general rule 0502 test of the 2015 edition of the Pharmacopoeia of the People's Republic of China, 5 μL of each of the above solutions was drawn, and they were respectively spotted on the same silica gel G thin-layer plate (20cm x 10cm, Merck Company, Germany), and mixed with cyclohexane-eth...
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