Use of grain worm or its extract in the preparation of medicines for reducing nephrotoxicity
A technology of grain worms and extracts, applied in the direction of drug combinations, pharmaceutical formulas, organic active ingredients, etc., can solve the problems of dose limitation, toxicity, and kidney production
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Embodiment 1
[0065] Embodiment 1: the preparation of grain worm extract
[0066] Prepare the grain worm extract according to the following method:
[0067] 1. Artificially cultured grain worms were freeze-dried, crushed and passed through a 60-mesh sieve, and then extracted with carbon dioxide fluid supercritically. The extraction parameters were pressure 25MPA, temperature 50°C, CO 2 The flow rate was 25 L / h, and the extraction was performed for 2 hours to obtain a crude product.
[0068] 2. Heat the crude product obtained in step 1 to 80°C, add 3% of the volume of the crude product in 80°C distilled water, stir for 30 minutes, and mix thoroughly; centrifuge at 5000r / min to remove the precipitate to obtain degummed grease.
[0069] 3. Take the degummed oil obtained in step 2, heat it to 60°C, add 0.3% (w / w) sodium hydroxide, stir for 1 hour, fine saponified matter appears, raise the temperature to 80°C, flocs appear and coagulate, 5000r / min centrifugal separation. Take the supernatant,...
Embodiment 2
[0071] Embodiment 2: the preparation of grain insect powder
[0072] Artificially cultured grain worms were freeze-dried, crushed and passed through a 100-mesh sieve for later use.
Embodiment 3
[0073] Embodiment 3: the preparation of the pharmaceutical composition comprising grain worm or its extract and riboflavin
[0074] Pharmaceutical composition 1: the ratio of five grain insect extract:riboflavin in the active ingredient is 120:1 (mass ratio).
[0075] Pharmaceutical composition 2: in the active ingredient, the ratio of grain worm extract:riboflavin is 100:1 (mass ratio).
[0076] Pharmaceutical composition 3: the ratio of five grain insect extract:riboflavin in the active ingredient is 80:1 (mass ratio).
[0077] Pharmaceutical composition 4: the ratio of five grain insect extract:riboflavin in the active ingredient is 50:1 (mass ratio).
[0078] Pharmaceutical composition 5: the ratio of five grain insect powder:riboflavin in the active ingredient is 100:1 (mass ratio).
[0079] Pharmaceutical composition 6: the ratio of five grain insect powder:riboflavin in the active ingredient is 80:1 (mass ratio).
[0080] Pharmaceutical composition 7: the ratio of fi...
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