Composition for anti-aging comprising Rhodobacter sphaeroides strain or culture solution of the strain
A technology for cosmetic composition and culture solution, applied in the directions of bacteria, microorganisms, microorganisms, etc., can solve problems such as incomplete research on interrelationships, and achieve the effects of preventing or improving skin wrinkles, enhancing skin barrier, and preventing or improving skin wrinkles
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Embodiment 1
[0048] Example 1. Isolation and species identification of Rhodobacter sphaericus strain
[0049] Water collected from Daehu Pond, a freshwater lake in Dangjin, Chungnam, Korea, was used as a culture medium, and the Rhodobacter sphaericus strain according to one embodiment was isolated therefrom and subjected to species identification. To isolate strains accordingly, prepare diluted R2A (DR2A) medium diluted with R2A media 1 / 10, and distribute the collected fresh water in an amount of about 100 μl, culture in an incubator at 25°C for about 3 days, and then isolate the corresponding strains therefrom . During the culturing, the culturing was performed under the condition of being irradiated with light using a fluorescent lamp and the condition of not irradiating light, and the Rhodobacter sphaericus strain was cultured under the condition of irradiating fluorescence, and isolated therein. The isolated strains were cultured using R2A medium for enrichment, and in order to iden...
Embodiment 2
[0050] The preparation of embodiment 2. rhodobacter sphaericus strain culture fluid
[0051] A culture solution of Rhodobacter sphaericus isolated in the above Example 1 was prepared.
[0052] The strain culture solution was prepared by performing enrichment culture in a 50 ml tube using the R2A medium. At this time, the enrichment culture was cultured by suspending the strain at 100 rpm / min, and cultured under the condition of continuously irradiating light with a fluorescent lamp. The temperature during the culturing was about 25°C, and the culturing was terminated after about 72 hours of culturing. Thereafter, the culture solution was sterilized by filtration using a 0.45 filter.
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