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Surface plasma resonance biochemical analyzer integrated with nucleic acid amplification reaction

A technology of surface plasma and biochemical analyzers, applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve the problems of immaturity, complicated operation, easy pollution costs, etc., and achieve high precision and simple mechanical structure , the effect of cost reduction

Pending Publication Date: 2020-03-24
BEIJING UNIV OF CHEM TECH
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  • Claims
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Problems solved by technology

Compared with PCR technology, isothermal amplification has the advantages of high specificity, fast analysis speed, low cost, no need for heating and cooling process, and low risk. However, the current domestic isothermal amplification technology is not very mature and needs further development.
[0007] Whether it is the traditional PCR amplification method or the emerging isothermal amplification or other amplification methods, the detection of corresponding amplification products usually uses gel electrophoresis detection or fluorescence quantitative detection methods. These traditional detection methods usually need to be detected Samples are marked, and there are problems such as complicated operation, easy pollution and high cost

Method used

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  • Surface plasma resonance biochemical analyzer integrated with nucleic acid amplification reaction
  • Surface plasma resonance biochemical analyzer integrated with nucleic acid amplification reaction
  • Surface plasma resonance biochemical analyzer integrated with nucleic acid amplification reaction

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Embodiment Construction

[0029] Below will combine in the present invention Figure 1 to Figure 5 , clearly and completely describe the technical solutions in the embodiments of the present invention, obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. The following description of an exemplary embodiment is merely illustrative in nature and is by no means intended to limit the invention and its application or uses. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0030] The relative arrangements of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise.

[0031] At the same time, it should be understood that, for the convenience of description, the sizes...

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Abstract

The invention discloses a surface plasmon resonance biochemical analyzer integrated with nucleic acid amplification reaction. The surface plasmon resonance biochemical analyzer integrated with nucleicacid amplification reaction is driven by only one motor to achieve SPR angle automatic adjustment function, angles of incident light and a light receiving device can be simultaneously changed between21.5 degrees and 75.5 degrees, and the angle range can be further expanded. The prism coupling type SPR technology is combined with nucleic acid PCR amplification or isothermal amplification or otheramplification technologies, and the two functions of nucleic acid amplification and SPR detection are achieved on one sensing chip; and a PCR amplification temperature control device is integrated with a SPR prism coupling optical detection device; a sensing chip and a micro-fluidic flow cell of a flow system adopt a separable design, so that biomolecules are fixed on the chip by an offline method and an online method. Corresponding nucleic acid amplification products are detected by adopting a prism coupling type SPR optical system, and fluorescence labeling of the amplification products isnot needed. The SPR detection sensitivity is high, so that the nucleic acid molecule amplification period is shortened, the traditional nucleic acid amplification time is shortened, and the detectionspeed is high.

Description

technical field [0001] The invention relates to a plasma resonance biochemical analyzer, which belongs to the technical field of polymerase chain reaction (PCR); in particular, it relates to a surface plasmon resonance biochemical analyzer integrating nucleic acid amplification reaction. The invention can simultaneously perform PCR reaction and The surface plasmon resonance (Surface Plasmon Resonance, SPR) reaction can also perform PCR or SPR alone. Application scenarios can study the interaction between biomolecules, such as molecular recognition, immune regulation, immunoassay, rapid detection of toxins and antibiotics, nucleic acid detection, proteomic drugs and screening, and real-time detection of related pharmacokinetics. Background technique [0002] SPR technology is a modern, advanced and general-purpose label-free biochemical detection technology developed in recent years. This technology is a biochemical detection technology based on physical optical phenomena, us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6844
CPCC12Q1/6844
Inventor 张璐璐李逸琛张浩邱宪波黄亚峰龚士淞
Owner BEIJING UNIV OF CHEM TECH
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