A method for detecting food intake of fruit flies

Abstract

The invention discloses a method for detecting the feed intake of fruit flies, which comprises the following steps: (1) cultivating the fruit flies to be tested using a first solid medium; (2) after the fruit flies complete the first cycle of cultivation , using the second solid medium to continue the second cycle of cultivation; (3) after completing the second cycle of cultivation, the fruit fly excrement is measured; wherein, the first solid medium contains brilliant blue dye, and the second solid medium Contains no dyes. The invention can accurately and continuously measure the feed intake of the fruit flies at multiple time points for a long time without harming the fruit flies.

Description

technical field [0001] The invention relates to the technical field of a method for detecting food intake of fruit flies. Background technique [0002] Drosophila is a commonly used model animal in biological research and is widely used in the fields of genetics, behavior, nutrition and pharmacology. The determination of Drosophila feed intake has always been an important experimental content in the study of Drosophila as a model animal, and it is an indispensable indicator in nutrition, behavior and drug research. [0003] In order to measure the feed intake of fruit flies, a variety of methods have been developed in the prior art. Among them, the mainstream methods adopted by domestic and foreign counterparts include: capillary method, dye method, radioisotope labeling method and observation record of feeding behavior There are several methods. Among them, the capillary method calculates the feed intake of fruit flies by injecting liquid medium into a glass capillary, an...

AI Technical Summary

Problems solved by technology

The dye method adds a specific non-absorbing dye to the food, transfers the eaten fruit flies into an EP tube with water, grinds them, makes the dye redissolve in water, and then measures the absorbance to indirectly reflect the food intake of the fruit flies , because the fruit flies will eliminate the dye in the body in a short time after eating, so this method can only measure the food intake of the fruit flies within 30 minutes, the random factor is large, the result variability is high, and it also needs to kill the fruit flies, so Unable to determine feed intake at multiple time points

Isotope labeling method by adding specific radioactive isotopes such as 32 P, the method of determining the accumulation level of radioactive isotopes in the body of fruit flies after eating is used to determine the feed intake, and its accuracy is easily affected by the nutritional composition of the medium and the sex difference of fruit flies, and the fruit flies also need to be killed for measurement. Therefore, it is also not suitable for the determination of feed intake at multiple time points

The feeding behavior observation and recording method reflects the food intake of fruit flies by manual observation or computer electrical signals recording the number of times the fruit flies' mouthparts come into contact with food within a period of time. It can only record the number of times fruit flies eat but cannot measure The volume of food consumed by the fly and is therefore not a true measure of feed intake

In summary, it can be seen that there is no method in the prior art that can realize the long-term and multi-time point feed intake of Drosophila, especially the accurate measurement of solid medium feed intake

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