A method for detecting food intake of fruit flies

A detection method and feed intake technology, applied in the field of animal husbandry, etc., can solve the problems of large random factors, high variability of results, not a method for measuring feed intake, etc., and achieve a simple and easy-to-obtain cultivation method with high controllability , low-cost effect

Active Publication Date: 2021-06-25
SICHUAN AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dye method adds a specific non-absorbing dye to the food, transfers the eaten fruit flies into an EP tube with water, grinds them, makes the dye redissolve in water, and then measures the absorbance to indirectly reflect the food intake of the fruit flies , because the fruit flies will eliminate the dye in the body in a short time after eating, so this method can only measure the food intake of the fruit flies within 30 minutes, the random factor is large, the result variability is high, and it also needs to kill the fruit flies, so Unable to determine feed intake at multiple time points
Isotope labeling method by adding specific radioactive isotopes such as 32 P, the method of determining the accumulation level of radioactive isotopes in the body of fruit flies after eating is used to determine the feed intake, and its accuracy is easily affected by the nutritional composition of the medium and the sex difference of fruit flies, and the fruit flies also need to be killed for measurement. Therefore, it is also not suitable for the determination of feed intake at multiple time points
The feeding behavior observation and recording method reflects the food intake of fruit flies by manual observation or computer electrical signals recording the number of times the fruit flies' mouthparts come into contact with food within a period of time. It can only record the number of times fruit flies eat but cannot measure The volume of food consumed by the fly and is therefore not a true measure of feed intake
In summary, it can be seen that there is no method in the prior art that can realize the long-term and multi-time point feed intake of Drosophila, especially the accurate measurement of solid medium feed intake

Method used

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  • A method for detecting food intake of fruit flies
  • A method for detecting food intake of fruit flies
  • A method for detecting food intake of fruit flies

Examples

Experimental program
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Effect test

Embodiment 1

[0057] as attached figure 1 The culture device shown, it comprises that an outer diameter W1 is 28.5mm, the inner diameter N1 is 26mm, and the round-bottomed transparent plastic fruit fly culture tube 1 that length is 130mm, there are a plurality of circumferential distributions on the upper part of the culture tube 1 with a diameter of 0.5 mm. The ventilation hole 101 of mm is used for the breathing of fruit flies; an inner diameter N2 is 23.5mm, and an outer diameter W2 is a large transparent plastic cover with an insertion hole 201 at the top of 25-26mm, that is, the tube cover 2; the tube cover 2 passes through its extension Insert in the culture tube 1 to realize the closure of the culture tube 1, a placer 3, the placer 3 includes an integrally formed transparent plastic small cover, that is, a device cover 301 and an inner diameter N3 of 5.5 mm and an outer diameter W3 of 7 mm. Cylindrical body 302 , the outer diameter W3 of the body 302 is the same as the diameter of th...

Embodiment 2

[0059] Using the cultivation device as described in Example 1, the food intake of Drosophila was detected, and the process was as follows:

[0060] (1) Two kinds of solid medium are prepared in advance, namely the first medium containing 1% brilliant blue dye and the second medium without dye, and the two kinds of medium are respectively filled into the bodies 302 of the two devices 3 ;

[0061] (2) Transfer the fruit flies to be tested to the fruit fly culture tube 1, cover the tube cover 2, and insert the first culture medium device 3 into the tube cover 2;

[0062] (3) After the fruit flies eat for 24 hours, a large amount of fruit fly excreta containing bright blue dye can be seen on the tube wall of the culture tube 1;

[0063] (4) The device 3 with the first culture medium is replaced with the device 3 with the second culture medium, and the fruit flies are fed with the dye-free medium for 3 hours, so as to discharge the residual blue dye in the body, and then It can c...

Embodiment 3

[0066] Using the detection process as described in Example 2, wherein the fruit flies to be tested are 10 in each culture tube, the making of the standard light absorption curve includes:

[0067] Dissolve 100 mg of brilliant blue dye in 10 mL of distilled water to prepare a mother liquor. Take 1mL of mother liquor into a 1.5mL EP tube (marked as No. 1), take 500uL solution from this EP tube into another EP tube (marked as No. 2), and add 500uL of distilled water to No. 2 tube at the same time, mix uniform. Take 500uL solution from No. 2 tube to No. 3 tube, then add 500uL distilled water to No. 3 tube, and mix well. In this cycle, the dye solution is gradually diluted. The corresponding Brilliant Blue dye concentration for each tube is as follows:

[0068] pipe number 12 11 10 9 8 7 6 5 4 3 2 1 Concentration (mg / mL) 0.00488 0.00976 0.0195 0.0391 0.0781 0.156 0.313 0.625 1.25 2.5 5 10

[0069] Add 100uL of each concentration soluti...

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Abstract

The invention discloses a method for detecting the feed intake of fruit flies, which comprises the following steps: (1) cultivating the fruit flies to be tested using a first solid medium; (2) after the fruit flies complete the first cycle of cultivation , using the second solid medium to continue the second cycle of cultivation; (3) after completing the second cycle of cultivation, the fruit fly excrement is measured; wherein, the first solid medium contains brilliant blue dye, and the second solid medium Contains no dyes. The invention can accurately and continuously measure the feed intake of the fruit flies at multiple time points for a long time without harming the fruit flies.

Description

technical field [0001] The invention relates to the technical field of a method for detecting food intake of fruit flies. Background technique [0002] Drosophila is a commonly used model animal in biological research and is widely used in the fields of genetics, behavior, nutrition and pharmacology. The determination of Drosophila feed intake has always been an important experimental content in the study of Drosophila as a model animal, and it is an indispensable indicator in nutrition, behavior and drug research. [0003] In order to measure the feed intake of fruit flies, a variety of methods have been developed in the prior art. Among them, the mainstream methods adopted by domestic and foreign counterparts include: capillary method, dye method, radioisotope labeling method and observation record of feeding behavior There are several methods. Among them, the capillary method calculates the feed intake of fruit flies by injecting liquid medium into a glass capillary, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 吴奇杨明耀余贵香毛雪平成星熠
Owner SICHUAN AGRI UNIV
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