Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of serum thymus factor in promoting proliferation of human amniotic mesenchymal stem cells in vitro and preparing proliferation culture medium

A technology of serum thymus factor and amniotic mesenchyme, which is applied in the direction of cell culture active agent, tissue culture, animal cells, etc., can solve the problem of promoting the proliferation of human amniotic mesenchymal stem cells without it.

Inactive Publication Date: 2020-03-31
南京温博生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Serum thymus factor is a nonapeptide in serum, and there is no report that it promotes the proliferation of human amniotic mesenchymal stem cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of serum thymus factor in promoting proliferation of human amniotic mesenchymal stem cells in vitro and preparing proliferation culture medium
  • Application of serum thymus factor in promoting proliferation of human amniotic mesenchymal stem cells in vitro and preparing proliferation culture medium
  • Application of serum thymus factor in promoting proliferation of human amniotic mesenchymal stem cells in vitro and preparing proliferation culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Serum thymus factors promote the proliferation of human amniotic mesenchymal stem cells in vitro

[0018] 1. Experimental materials

[0019] D-Hanks solution was purchased from Guangzhou Peiyu Biological Products Co., Ltd.

[0020] Trypsin was purchased from Shanghai Hengdu Biotechnology Co., Ltd.

[0021] Collagenase type II was purchased from Abisson (Shanghai) Biotechnology Co., Ltd.

[0022] DMEM / F12 medium, FBS and penicillin and streptomycin were purchased from Gibco.

[0023] The fluorescently labeled flow antibodies used in flow cytometric identification are products of Beckman.

[0024] Serum thymus factor (CAS No. 63958-90-7) was purchased from Shanghai Yuanye Biotechnology Co., Ltd., with a purity of ≥95%.

[0025] 2. Experimental method

[0026] 1. Cell isolation, culture and identification

[0027] Peel off the amniotic membrane on the fetal surface of the placenta under aseptic conditions, wash it repeatedly with D-Hanks solution pre-coole...

Embodiment 2

[0053] Example 2: Human amniotic mesenchymal stem cell culture medium

[0054] A cell culture medium for improving the proliferation efficiency of human amniotic mesenchymal stem cells is prepared by adding an effective concentration of serum thymus factor to DMEM / F12 medium.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of serum thymus factors in promoting proliferation of human amniotic mesenchymal stem cells in vitro and preparing a proliferation culture medium. The present invention finds that the serum thymus factor can effectively promote the proliferation of the human amniotic mesenchymal stem cells; obvious dose dependence and time dependence are shown; and the serumthymus factor intervened culture does not influence the multidirectional differentiation capacity of the human amniotic mesenchymal stem cells, so that the serum thymus factor with effective concentration can be added into a human amniotic mesenchymal stem cell culture medium to prepare the culture medium for improving the proliferation efficiency of the human amniotic mesenchymal stem cells.

Description

technical field [0001] The invention belongs to the field of biology and relates to stem cell culture, in particular to the use of serum thymus factor to promote the proliferation of human amniotic mesenchymal stem cells in vitro and to prepare a proliferation medium. Background technique [0002] Amniotic mesenchymal stem cells are a type of stem cells derived from amniotic membrane. Compared with stem cells from other sources, amniotic mesenchymal stem cells have low immunogenicity and immunosuppressive characteristics, which makes the transplantation of amniotic mesenchymal stem cells have less side effects. Amniotic mesenchymal stem cell transplantation has achieved good results in the treatment of some inflammation-related diseases, and relevant clinical trials are being carried out gradually. [0003] In order to obtain a large number of amniotic mesenchymal stem cells in a short period of time, it is necessary to culture them in vitro, and the in vitro proliferation ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2501/999
Inventor 姜东花其他发明人请求不公开姓名
Owner 南京温博生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com