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Method for rapid propagation of embryogenic cells of fraxinus mandshurica

An embryonic cell, fast technology, applied in the field of cell engineering, can solve problems such as unfavorable sustainable development, destruction, waste of resources, etc.

Active Publication Date: 2020-04-03
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, trees will die after being barked, and cutting down will also cause waste and destruction of resources, which is not conducive to sustainable development

Method used

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  • Method for rapid propagation of embryogenic cells of fraxinus mandshurica
  • Method for rapid propagation of embryogenic cells of fraxinus mandshurica
  • Method for rapid propagation of embryogenic cells of fraxinus mandshurica

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Embodiment Construction

[0018] The present invention will be described in detail below in combination with specific embodiments.

[0019] The present invention adopts WPM as the base medium, adds 30g / L sucrose, and four kinds of hormones with different concentrations:

[0020] Solid medium: WPM basic medium + sucrose 30g / L + BA + NAA + TDZ + IBA + agar 4.5g / L, pH 5.8-6.0;

[0021] Liquid medium: WPM basic medium + sucrose 30g / L + BA + NAA + TDZ + IBA, pH 5.8-6.0;

[0022] Hormone concentrations are shown in Table 1 below:

[0023] Table 1 Hormone concentration

[0024]

[0025] The present invention carries out according to the following steps:

[0026] Optimization of sucrose concentration:

[0027] Add different concentrations of sucrose, the sucrose concentration is 10g / L, 20g / L, 30g / L, 60g / L, 80g / L, culture for 20d, collect the cells, and weigh their growth. According to the concentration of sucrose, the growth rate of cell dry weight changes as 30g / L>20g / L>10g / L>60g / L>80g / L, therefore, 3...

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Abstract

The invention discloses a method for rapid propagation of embryogenic cells of fraxinus mandshurica. The method comprises the following steps of selecting calluses of fraxinus mandshurica, wherein thecalluses can be originated from various explants such as roots, stems, leaves, seeds and embryos, and inoculating the calluses to an F4 solid medium to cultivate 1-2 subcultures so as to obtain embryogenic calluses of fraxinus mandshurica; and inoculating friable embryogenic calluses to an F4 liquid medium to cultivate 2-3 subcultures so as to obtain single cells and small cell groups of fraxinusmandshurica. The method has the advantages that the F4 media are selected; besides conventional WPM basic media, 30g / L saccharose is selected to be combined with four hormones: BA, NAA, IBA and TDZ;cells on the media grow vigorously, and no death is caused by browning as long as the subculturing takes place in the growth cycle. The method is established to lay a foundation for scaled productionof cells of fraxinus mandshurica and researches on secondary metabolites.

Description

technical field [0001] The invention relates to a method for rapidly propagating embryogenic cells of Mandshurica mandshurica, belonging to the technical field of cell engineering. Background technique [0002] Fraxinus mandschurica Rupr. Oleaceae, Ash genus, tall deciduous tree. The basic features are: thick bark, taupe, large winter buds, conical shape, thick branchlets, quadrangular shape, leaf scars nodular uplift, semicircle. Pinnate compound leaves; petiole enlarged near the base, leaf inserts jointed, papery, oblong to ovate-oblong, leaf margin finely serrated, dark green above, yellow-green below, panicles born last year On the growing branches, the first leaves are open, the peduncle and branches have narrow wing-like sharp edges; male flowers and hermaphroditic flowers are dioecious, neither corolla nor calyx; male inflorescences are compact, pedicels are thin and short, anthers are oval, filaments are very short, seeds The room is flat and wide, and the samara i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 齐凤慧詹亚光陈思齐刘林曾凡锁
Owner NORTHEAST FORESTRY UNIVERSITY
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