Method for rapidly identifying cadmium accumulation of rice by using in-vitro rice ears

Abstract

The invention discloses a method for rapidly identifying cadmium accumulation of rice by using in-vitro rice ears. The method comprises the following steps: soaking the in-vitro rice ears in nutrientsolutions with different cadmium ion concentrations for 48 hours, carrying out fixation and drying on the in-vitro rice ears, determining the cadmium content in the in-vitro rice ears, recording the change of the cadmium ion flow rate of the in-vitro rice ears within 10 minutes, preparing the flow rate curve graph of in-vitro rice ear samples to be detected, and judging the low-cadmium accumulation variety of the rice according to the flow rate curve graph of the in-vitro rice ear samples. According to the method, the difference of cadmium accumulation capacities in different varieties of ricecan be accurately identified within a short time on the basis of the flow velocity of cadmium ions entering rice ears of rice development, and the method has the advantages of high efficiency, good repeatability and the like; and moreover, according to the cadmium ion flow rate of developed rice ears, genotypes with low-cadmium accumulation characteristics can be screened out before rice maturation, so that time and labor can be effectively saved, the screening efficiency of excellent low-cadmium genotypes is greatly improved, and the screening process of low-cadmium rice varieties is accelerated.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for quickly identifying rice cadmium accumulation by using isolated rice ears. Background technique [0002] Cadmium (Cd) is one of the most toxic heavy metals. At present, the area of ​​heavy metal pollution in my country's cultivated land accounts for about 1 / 6 of the total cultivated land, and the area polluted by Cd far exceeds that of other heavy metal elements. Rice is one of the food crops widely grown in my country and even in the world, and rice has a strong ability to absorb and enrich Cd in polluted environments. The phenomenon of "cadmium rice" that has appeared frequently in recent years has also made all sectors of society pay more attention to the selection of low-cadmium-accumulating rice varieties. The breeding of rice with low cadmium accumulation has always been the research focus of scientists. At present, some rice varieties with low c...

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Problems solved by technology

However, since the cadmium accumulation in rice is easily affected by many uncontrollable factors such as plant development, soil organic matter, and water management, there are very few opportunities to screen for genetic variation, and the process of screening rice varieties with low cadmium accumulation is very slow.

[0003] The traditional method is to plant rice varieties in heavy metal-polluted farmland, and select low-cadmium-accumulating varieties according to the analysis results of cadmium content in mature rice, which is easily affected by the uniformity of cadmium content in the field soil and the growth status of plants, irrigation quality, and transpiration. and other environmental factors, and the test time is long, the required materials are large, and the work efficiency is low

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