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fut2 gene expression product related glycan detection kit

A detection kit and detection line technology, applied in the field of FUT2 gene expression product-related glycan detection kits, to achieve high sensitivity

Active Publication Date: 2020-08-21
BIOSTIME GUANGZHOU HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection kit of Patent Document 2 has problems of further improving sensitivity and preventing false positives
Therefore, there is room for improvement in the detection method disclosed in Patent Document 2

Method used

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  • fut2 gene expression product related glycan detection kit

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0023] Preparation Example 1: Preparation of Kit

[0024] The upper surface of the PVC base plate is fixedly arranged as the nitrocellulose membrane of the supporting membrane, and the two sides of the nitrocellulose membrane are respectively provided with a colloidal gold bonding pad and a water-absorbing pad; The upper surface of the left end is fixedly connected, and the remaining lower surface of the colloidal gold bonding pad is fixedly connected with the upper surface of the PVC bottom plate; the lower surface of the left end of the water-absorbing pad is fixedly connected with the upper surface of the right end of the nitrocellulose membrane, and the remaining lower surface of the water-absorbing pad is connected with the PVC bottom plate The upper surface of the colloidal gold bonding pad is fixedly connected; a sample pad is also provided on the left side of the colloidal gold bonding pad, the lower surface of the right end of the sample pad is fixedly connected with t...

preparation example 2

[0031] Preparation Example 2: Preparation of Kit

[0032] Similar to the above Preparation Example 1, the difference is that 20 microliters of TiO with an average particle size of 12 μm at a concentration of 1 mg / 1 mL 2 The microspheres were dropped onto the sample pad and allowed to air dry.

[0033] Detection example

[0034] Collection of samples to be tested

[0035] Subject mothers: 5 healthy pregnant women (number #1~#5) who were 34 weeks pregnant were selected from Dalian Maternal and Child Health Hospital and the Department of Obstetrics and Gynecology of Suihua No. It mainly includes age, physical signs, maternal history, current and previous health history, dietary habits and physical activity level, and history of medication and supplement intake.

[0036]Breast milk collection: sample tubes were given to subjects at different times, and the date of collection, time of eating and drinking before sample collection and content of food were recorded. The sample col...

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Abstract

The invention relates to a detection kit for an FUT2 gene expression product. The detection kit is characterized by comprising fucosan binding protein modified colloidal gold and titanium dioxide microspheres. The fluorescence detection kit for the FUT2 gene expression product can detect a target product with high sensitivity.

Description

technical field [0001] The invention relates to a detection kit for glycans related to FUT2 gene expression products with high sensitivity. Background technique [0002] The colonization of beneficial intestinal flora and the establishment of healthy flora structure in neonates significantly affect their growth and development. Studies have shown that the proportions of normal flora in the intestinal tract of newborns are: Lactobacillus 1%, Bifidobacteria 60%-90%, and other bacteria 10%-40%, among which Bifidobacterium spp. occupies an absolute dominant position . Bifidobacterium is a physiological anaerobic bacterium that colonizes early in the intestinal tract of infants. It is the dominant bacterium in the normal intestinal flora. play a positive role in many other aspects. Therefore, the colonization and diversity of bifidobacteria in the intestinal tract of infants is of great significance to ensure the health of infants and young children. [0003] "Science" and "C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/573
CPCG01N33/573G01N33/577G01N2333/91091
Inventor 马博轩刘斐童邓超明陈桔淳
Owner BIOSTIME GUANGZHOU HEALTH PROD