A kind of subculture method of showing pulse fragrant tea vegetable
A technology of subculturing and subculturing of fenugreek, which is applied in the field of plant tissue culture, can solve the problems of vitrification and low multiplication coefficient, and achieve the effects of reducing serious vitrification, high multiplication coefficient and reducing production cost.
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Embodiment 1
[0017] A kind of subculture method of showing veins fragrant tea dish, concrete steps are:
[0018] (1) Selection of aseptic seedlings: select the aseptic seedlings of Xianmaixiang tea vegetables with neat growth, 2-3cm plant height, and 5-7 days of cultivation as subculture materials from the starting medium;
[0019] (2) Subculture of sterile vaccines: transfer the sterile vaccines to the subculture medium for multiple subcultures. The composition of the proliferation medium is MS+6-BA 0.5mg / L+white sugar 30g / L+ Agar 5 / L, pH 5.8. The whole cultivation process was carried out in the plant tissue culture room, and was irradiated by fluorescent lamps, the illumination time was 12h / d, the illumination intensity was 2500lx, and the cultivation temperature was 24°C. The proliferation medium was sterilized by autoclaving at 121°C for 20 minutes.
[0020] After cultivating for 25 days, the proliferating mother seedlings with no roots, many shoots and side branches and a high proli...
Embodiment 2
[0022] A kind of subculture method of showing veins fragrant tea dish, concrete steps are:
[0023] (1) Selection of aseptic seedlings: select the aseptic seedlings of Xianmaixiang tea vegetables with neat growth, 2-3cm plant height, and 5-7 days of cultivation as subculture materials from the starting medium;
[0024] (2) Subculture of sterile seedlings: Transfer the sterile seedlings to the subculture medium for multiple subcultures. The composition of the proliferation medium is MS+6-BA 1mg / L+white sugar 28g / L+agar 6 / L with a pH of 5.6. The whole cultivation process was carried out in the plant tissue culture room, and the sunlight was irradiated. The light time was 10 h / d, the light intensity was 2600 lx, and the culture temperature was 25°C. The proliferation medium was sterilized by autoclaving at 122°C for 21 minutes.
[0025] After 26 days of cultivation, the proliferating mother seedlings with no roots, many shoots and side branches, and a high proliferation ratio w...
Embodiment 3
[0027] A kind of subculture method of showing veins fragrant tea dish, concrete steps are:
[0028] (1) Selection of aseptic seedlings: select the aseptic seedlings of Xianmaixiang tea vegetables with neat growth, 2-3cm plant height, and 5-7 days of cultivation as subculture materials from the starting medium;
[0029] (2) Subculture of sterile vaccines: transfer the sterile vaccines to the subculture medium for multiple subcultures. The composition of the proliferation medium is MS+6-BA 1.5mg / L+white sugar 32g / L+ Agar 6 / L, pH 6. The whole cultivation process was carried out in the plant tissue culture room, under the irradiation of fluorescent lamp, the illumination time was 14h / d, the illumination intensity was 2700lx, and the cultivation temperature was 23°C. The proliferation medium was sterilized by autoclaving at 120°C for 18 minutes.
[0030] After cultivating for 30 days, the proliferating mother seedlings with no roots, many shoots and side branches and a high proli...
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