Application of DPCPX inhibitor in preparation of PD-L1/PD-1 monoclonal antibody tumor immunotherapy drug

A technology of PD-L1 and anti-tumor immunity, which is applied in the field of PD-L1/PD-1 monoclonal antibody tumor immunotherapy and biology, and can solve problems such as unclear mechanism of action

Active Publication Date: 2020-04-10
XIANGYA HOSPITAL CENT SOUTH UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In innate immunity, ATF3 interacts with NF-κB and pro-inflammatory cytokines IL-6, IL-12b, toll-like receptor-4 (TLR-4) as an immunomodulator in LPS-treated mice, but Its exact mechanism of action in the immune response remains unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of DPCPX inhibitor in preparation of PD-L1/PD-1 monoclonal antibody tumor immunotherapy drug
  • Application of DPCPX inhibitor in preparation of PD-L1/PD-1 monoclonal antibody tumor immunotherapy drug
  • Application of DPCPX inhibitor in preparation of PD-L1/PD-1 monoclonal antibody tumor immunotherapy drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. In vivo study on the proliferation of adenosine receptor ADORA1 combined with PD-1 monoclonal antibody against transplanted tumor (melanoma) in mice.

[0040] 1.1 Group settings:

[0041] Scramble+IgG2a (control group)

[0042] shAdora1+IgG2a (Adora1 knockdown group)

[0043] Scramble+PD-1mAb (PD-1 mAb treatment group)

[0044] shAdora1+PD-1mAb (joint intervention group)

[0045] 1.2 Experimental process, see figure 1 A:

[0046] About 6 days before the experiment: 6-8 weeks old C57BL / 6 mice were subcutaneously injected with B16F10 melanoma shAdora1 knockdown cell line 5*10 in the right dorsal wing. 5 20 mice each and the same number of Scramble cell lines (control group).

[0047] Day 0 of the experiment: record the body weight and tumor volume of the mice, when the tumor volume grows to about 100mm 3 Beginning at the beginning of time, the mice inoculated with the shAdora1 knockdown cell line and the Scramble cell line were injected intraperitoneally with PD...

Embodiment 2

[0063] 1. The in vivo study of the specific inhibitor DPCPX targeting and inhibiting the proliferation of adenosine receptor ADORA1 combined with PD-1 monoclonal antibody transplanted tumors (melanoma, non-small cell lung cancer) in mice.

[0064] 1.1 Group settings:

[0065] Vehicle+IgG2a (control group)

[0066] DPCPX+IgG2a (Adora1 inhibition group)

[0067] Vehicle+PD-1mAb (PD-1 mAb treatment group)

[0068] DPCPX+PD-1mAb (joint intervention group)

[0069] 1.2 Experimental process, see image 3 A. 4A:

[0070] About 6 days before the experiment: 6-8 weeks C57BL / 6 mice were subcutaneously injected with B16F10 melanoma 5*10 in the right dorsal wing 5 Individual or LLC non-small cell lung cancer 1*10 6 wild-type cell lines, 40 mice each.

[0071] Day 0 of the experiment: record the body weight and tumor volume of the mice, when the tumor volume grows to about 100mm 3 At the beginning of time, the inoculated mice were divided into four groups, respectively, DPCPX 1mg / K...

Embodiment 3

[0093] 1. In vivo study of gene knockdown of transcription factor ATF3 combined with adenosine receptor ADORA1 specific inhibitor DPCPX against proliferation of mouse xenograft tumors (melanoma, non-small cell lung cancer).

[0094] 1.1 Group settings:

[0095] Scramble+vehicle (control group)

[0096] Scramble+DPCPX (Adora1 inhibition group)

[0097] ShAtf3+vehicle (Atf3 knockdown group)

[0098] ShAtf3+DPCPX (joint intervention group)

[0099] 1.2 Experimental process, see Figure 5 A. 6A:

[0100] About 6-8 days before the experiment: 6-8 weeks old C57BL / 6 mice were subcutaneously injected with B16F10 melanoma shAtf3 knockdown cell line 5*10 in the right dorsal wing respectively. 5 Individual or LLC non-small cell lung cancer shATF3 knockdown cell line 1*10 6 , and the same number of Scramble cell lines (control group), 20 mice each.

[0101] Day 0 of the experiment: record the body weight and tumor volume of the mice, when the tumor volume grows to about 100mm 3 Be...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an application of a DPCPX inhibitor in preparation of a PD-L1/PD-1 monoclonal antibody tumor immunotherapy drug, specifically, the DPCPX inhibitor is used for preparing the PD-L1/PD-1 monoclonal antibody tumor immunotherapy drug by targeted inhibition of an adenosine receptor ADORA1, and also provides an application of combination of the DPCPX inhibitor and the PD-L1/PD-1 monoclonal antibody drug in preparation of a tumor immunotherapy adjuvant drug. The invention also provides an application of ATF3 as a target site detection reagent in combination with the DPCPX inhibitor in preparation of the PD-L1/PD-1 monoclonal antibody tumor immunotherapy drug. The tumor is a solid tumor, preferably melanoma or lung cancer, and further preferably melanoma or non-small cell lung cancer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of PD-L1 / PD-1 monoclonal antibody tumor immunotherapy. Background technique [0002] In recent years, immune checkpoint blockade (Immune checkpoint blockades, ICB)-related therapies have been widely studied by scientists around the world, and have been used for some malignant tumors, such as malignant melanoma (MM) and non-small cell lung cancer (nonsmall cell lung cancer). lung cancer (NSCLC) has brought enormous clinical benefits. Among them, the clinical application of neutralizing antibodies that block the interaction between programmed cell death protein 1 (PD-1) and its ligand (PD-L1) in multiple tumors has brought breakthroughs in tumor immunotherapy. However, with the deepening of the research, the researchers found that it still has obvious shortcomings. Only less than 40% of tumor patients respond to PD-L1 / PD-1 blocking therapy, or there are primary or secondary ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K39/395A61P35/00A61P37/04
CPCA61K39/3955A61K45/00A61K2039/505A61P35/00A61P37/04
Inventor 陈翔刘洪匡欣薇
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap