Primer groups and kit for detecting ABO genotypes of human red blood cells, and application of kit

Abstract

The invention discloses primer groups and a kit for detecting ABO genotypes of human red blood cells, and application of the kit in reagents. 24 primer groups are disclosed in the invention, and the sequence table of the 24 primer groups is as shown in SEQ ID No. 1-72. The kit comprises at least 22 primer groups selected from the 24 primer groups. The reagents comprise the 24 group primer groups or the kit. The primers are optimally designed and have similar annealing temperatures, so the 24 groups of primers can be simultaneously amplified under the same PCR amplification condition; the volume of each PCR reaction system is only 1.15 mu L, and only 5-10 ng of a DNA template is needed, so detection efficiency and reaction sensitivity are greatly improved; and the primer groups have the advantages of high speed, low cost, high sensitivity, easy realization of multi-site joint detection and the like when used for detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer set, a kit and an application for detecting human erythrocyte ABO blood type genotyping. Background technique [0002] Since the famous Austrian medical scientist Karl Landerstainer discovered the human ABO blood group system in 1900, there are currently 39 blood group systems recognized by the International Society of Blood Transfusion (ISBT). The ABO blood group system is the most immunogenic blood group system in the human blood group system, and it is also a classic human genetic marker, so it plays an important role in blood transfusion medicine, paternity testing, anthropological research, and forensic evidence testing. [0003] The human ABO gene is located on chromosome 9 (9q34.1-9q34.2), controlled by three multiple alleles A, B and O, including 7 exons ranging in length from 28bp to 691bp and 6 exons of about 19514bp in length. An intron, its DNA sequence is highl...

AI Technical Summary

Problems solved by technology

The PCR-SSP method requires multi-tube amplification and multi-band electrophoresis, and there is also the risk of mistyping caused by confusing interpretation

The biggest defect of the PCR-SBT typing method is that the operation process is very cumbersome, the workload is heavy, and it is difficult to achieve high-throughput operation

The amplification of different regions of the ABO gene has different amplification conditions, which not only requires a large sample size, but also increases the consumption of reagent consumables, and requires more equipment resources

To sum up, the current common methods have the following problems: the first point is that the experimental operation is complicated and the steps are cumbersome; the second point is that the result interpretation is still manual; the third point is that the sample throughput is low; the fourth point is that the cost is relatively high. high

These defects directly lead to the inability of ABO genotyping to achieve high-throughput accurate typing, and it is difficult to adapt to the increasing medical needs in the future

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